A Review of Gene Expression in Porcine Endometrial Lymphocytes, Endothelium and Trophoblast During Pregnancy Success and Failure

Tayade, Chandrakant; Yuan, Fang; Croy, B. Anne

doi:10.1262/jrd.18170

Cited by 49 publications

(47 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the lack of direct contact of the myometrium with embryonic secretes and weak myometrial production of estrogens during early pregnancy may result in local "downregulation" of OTR mRNA in the myometrium. Moreover, growth factors and cytokines secreted by embryos during the periimplantation period [59][60][61] indicate a possible influence on OTR gene expression in uterine tissues.…”

Section: Discussionmentioning

confidence: 99%

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Franczak

Bogacki

2009

J. Reprod. Dev.

View full text Add to dashboard Cite

Abstract. In the pig, the periimplantation period is critical for successful establishment of pregnancy. We studied the influence of embryos on oxytocin (OT) and progesterone (P4) regulated endometrial and myometrial secretion of 1) luteotrophic prostaglandin E2 (PGE2) and 2) luteolytic prostaglandin F2α and its metabolite (PGFM) on days 12-14 of pregnancy in pigs. We used unilaterally pregnant pigs created by a surgical procedure in which one uterine horn remained intact and the second horn was cut transversely so that part of the horn was detached from the uterine body. The animals were divided into two groups, inseminated gilts (days 12-14 of pregnancy, n=6) and uninseminated cyclic gilts, which were used as controls (days 12-14 of estrous cycle, n=5). Embryos developed only in the patent part of the uterus and not in the occluded horn. The abundance of OTR mRNA was increased in the endometrium and decreased in the myometrium of the gravid uterine horn in the pregnant pigs compared with the non-gravid uterine horn or either uterine horn in the cyclic pigs, indicative of a local effect of the conceptus. The presence of embryos in the uterine horn during the periimplantation period determines endometrial metabolism of PGF2α and the local response of the endometrium to OT and P4. OT stimulates PGF2α secretion and PGFM accumulation in endometrial cultures only from the non-gravid uterine horn and controls PGE2 secretion from the endometrium and myometrium in the pregnant gilts. The results indicate a more systemic affect of pregnancy on the uterine response to OT and a possibly the local effect of the conceptus in promoting progesterone's inhibition of OT-stimulated PGE2 secretion and uterine metabolism of PGF2α. Key words: Embryos, Oxytocin, Pigs, Progesterone, Prostaglandin E2, Prostaglandin F2α and its metabolite, Uterus (J. Reprod. Dev. 55: [262][263][264][265][266][267][268][269][270][271][272] 2009) rostaglandins (PG) produced in the uterus and corpus luteum are necessary for successful reproduction [1,2]. Oxytocin, acting through oxytocin receptors (OTR) in the endometrium and myometrium, is involved in the control of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) secretion in pigs [3][4][5][6][7][8]. Prostaglandin F2α is responsible for luteal regression in non-pregnant pigs [1,9], while prostaglandin E2 acts as a luteotrophic and antiluteolytic factor that promotes maintenance of the corpus luteum during early pregnancy in pigs [10,11].Alteration of PG secretion by OT is implicated in maternal recognition of pregnancy in pigs [12][13][14]. Moreover, OT stimulates myometrial secretion of PGF2α and PGE2 as well as the contractile activity of the porcine myometrium during early pregnancy [8,15,16]. The uterine response to OT depends on expression of OTR mRNA and protein [7,[17][18][19] and on generation of the second messengers inositol triposhate and diacyloglycerol [7,18,20,21]. OT also stimulates PGF2α secretion from the bovine endometrium through a previously discussed signaling pathway [22][2...

show abstract

Section: Discussionmentioning

confidence: 99%

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Franczak

Bogacki

2009

J. Reprod. Dev.

View full text Add to dashboard Cite

show abstract

“…Several hypotheses related to potential functions of these cells are raised by detailed studies conducted in humans and mice, species that have hemochorial placentas (TAYADE et al, 2007). In this study bovines showed greater expression of CD335+ cells during the end of pregnancy.…”

Section: Discussionmentioning

confidence: 71%

Immunophenotyping of leukocytes in bovine placenta

Chucri

Monteiro

Lima

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

Lymphocytes and macrophages are the main white cells involved in fetal-maternal tolerance. Little is known about these leukocytes in bovine placenta, such as the quantity and location of these cells. Thus, the objective of this study was to identify lymphocyte and macrophage populations in bovine placenta using specific markers and flow cytometry. This study analyzed samples of placentomes and intercaruncular regions of cows in the three quarters of pregnancy. In the placentomes, during the first quarter of pregnancy, mean percentage of labeled CD3+ cells was 2.34%; CD8+, 1.28%; CD14+, 1.66%; and CD335+, 0.96%. For the intercaruncular region, percentage of CD3+ cells was 0.71%; CD8+, 1.63%; CD14+, 2.81%; and CD335+, 2.81%. In the second quarter, placentomes showed 0.94% CD3+ cells; 0.77% CD8+; 0.72% CD14+; and 0.51% CD335+. In the intercaruncular region, percentage of labeled CD3+ cells was 0.50%; CD8+, 1.81%; CD14+, 2.64%; and CD335+, 0.51%. In the third quarter, placentomes showed labeling of 0.88% CD3+; 0.66% CD8+; 1.06% CD14+; and 0.74% CD335+ cells. In the intercaruncular region, percentage of labeled CD3+ cells was 0.19%; CD8+, 2.23%; CD14+, 2.43%; and CD335+, 0.16%. The results showed that there was a greater immunomarking of leukocytes CD3+ and CD335+ in the placentome when compared to the intercaruncular region during the third trimester. It can be concluded that leukocytes populations in bovine placenta is reduced, probably because of the syndesmochorial characteristic of bovine placenta. This represents a significant barrier for the immunological system of the mother, sharply decreasing the exposure of the conceptus to the mother's immune system. Keywords: Placentome. Intercaruncular region. Immunology. Maternal-fetal tolerance. ResumoLinfócitos e macrófagos são os principais leucócitos envolvidos na tolerância materno-fetal. Pouco se sabe sobre esses leucócitos na placenta bovina, como por exemplo, a quantidade e localização dessas células. Assim, o objetivo desse estudo foi identificar populações de linfócitos e macrófagos na placenta bovina utilizando marcadores específicos e citometria de fluxo. Este estudo analisou amostras de placentônios da região intercaruncular de bovinos nos três trimestres da gestação. No primeiro trimestre, nos placentônios, a porcentagem média de células CD3+ foi 2,34%; CD8+, 1,28%; CD14+, 1,66%; e CD335+, 0,96%. Na região intercaruncular, a porcentagem de células CD3+ foi 0,71%; CD8+, 1,63%; CD14+, 2,81%; e CD335+, 2,81%. No segundo trimestre, os placentônios apresentaram 0,94% de células CD3+; 0,77% de CD8+; 0,72% de CD14+e 0,51% de CD335+. Na região intercaruncular, a porcentagem de células CD3+ foi 0,50%; CD8+, 1,81%; CD14+, 2,64%; e CD335+, 0,51%. No terceiro trimestre, os placentônios apresentaram 0,88% de células marcadas CD3+; 0,66% de CD8+; 1,06% de CD14+ e 0,74% de CD335+. Na região intercaruncular, a porcentagem de células CD3+ foi 0,19%; CD8+, 2,23%; CD14+, 2,43% e CD335+, 0,16%. Os resultados mostraram que a imunomarcação de leucócitos na região do pl...

show abstract

“…Any factors resulting in angiogenesis blocked in final would induce fetus loss in many species including humans, pigs, mice (Tayade et al, 2007). Accordingly, whether Curcuma zedoaria causes it through inhibiting angiogenesis is worth to be investigated.…”

Section: Discussionmentioning

confidence: 99%

Anti-angiogenesis effect of essential oil from Curcuma zedoaria in vitro and in vivo

Chen

Gao

et al. 2011

Journal of Ethnopharmacology

View full text Add to dashboard Cite

A Review of Gene Expression in Porcine Endometrial Lymphocytes, Endothelium and Trophoblast During Pregnancy Success and Failure

Cited by 49 publications

References 44 publications

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Immunophenotyping of leukocytes in bovine placenta

Anti-angiogenesis effect of essential oil from Curcuma zedoaria in vitro and in vivo

Contact Info

Product

Resources

About