A Review of Mycotoxins in Food and Feed Products in Portugal and Estimation of Probable Daily Intakes

Abrunhosa, Luı́s; Morales, Héctor; Soares, Célia Maria Gonçalves; Calado, Thalita; Vila-Chã, A. Sofia; Pereira, Martinha; Venâncio, Armando

doi:10.1080/10408398.2012.720619

Cited by 124 publications

(78 citation statements)

References 84 publications

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“…This result agrees with other publications where low levels of OTA were also detected in Portuguese wines (Festas et al, 2000;Pena, Cerejo, Silva, & Lino, 2010;Ratola, Martins, & Alves, 2004). According to Abrunhosa et al (2016), the levels found by several authors range from 0.002 to 2.4 µg L −1 , with only two wines having OTA above the EU legal limit.…”

Section: Application Of Bsa-agarose Spe Of Ota Determination In Winessupporting

confidence: 92%

“…Wine is a widely consumed beverage and represents one of the biggest sources of OTA daily intake in some countries (Miraglia & Brera, 2002). For example, in Portugal, wine contributes for 8% of the probably daily intake of OTA (Abrunhosa et al, 2016). Thus, the European Commission fixed the regulatory limit of OTA in wine, grape juices and grape beverages at 2 μg Kg −1 (EC, 2016).…”

Section: Introductionmentioning

confidence: 99%

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BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

et al. 2019

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A B S T R A C TAnalytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R 2 = 0.9999) in the OTA concentration range studied (0.05 to 3.0 μg L −1 ), with recovery rates above 98%. It also showed low detection (0.017 μg L −1 ) and quantification (0.051 μg L −1 ) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results.

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Section: Application Of Bsa-agarose Spe Of Ota Determination In Winessupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

et al. 2019

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“…Analytical techniques such as thin-layer chromatography [6], high-performance liquid chromatography (HPLC) [7][8][9][10], and enzyme-linked immunosorbent assay (ELISA) have been available for the qualitative and quantitative analysis of AFB2 [11]. Although HPLC is the most commonly used method, because it offers high sensitivity and selectivity, it has some limitations in terms of costs incurred by the sophisticated equipment and the methods are not cost-effective, requiring a relatively long analysis time, so they are neither readily available in developing countries nor capable of on-site detection.…”

Section: Introductionmentioning

confidence: 99%

Visual and microplate detection of aflatoxin B2 based on NaCl-induced aggregation of aptamer-modified gold nanoparticles

et al. 2014

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We describe a fast and sensitive method for the detection of aflatoxin B2 (AFB2) that is based on the addition of AFB2-sensitive aptamers to a colloidal solution of gold nanoparticles (AuNPs). Subsequent addition of AFB2 and NaCl to the solution causes a color change from wine-red to blue-purple. Best results are obtained at a pH value of 7.0, a 10 mM aptamer concentration, and a 2 M concentration of NaCl. The method has a linear dynamic range that extends from 0.025 to 10 ng mL −1 of AFB2, and the detection limit is 25 pg mL −1 . The method is simple, fast, highly sensitive, and enables both visual and microplate readout.

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“…Mycotoxins occur particularly in regions or countries with climates of high temperature and humidity or where there are poor crop harvesting and storage conditions, which encourage mould growth and mycotoxin development (25). The toxic effects of mycotoxins are mainly on liver and they cause teratogenic, mutagenic, immunosuppressive, carcinogenic, cytotoxic, neurotoxic, nephrotoxic and oestrogenic effects (3,21). These toxins may show no clinical symptoms unless they are with secondary bacterial infections and high rate of mortality.…”

Section: Introductionmentioning

confidence: 99%

Investigation of mycotoxin residues in poultry feeds by LC MS/MS method

Yalçın¹,

Işık²,

Avci³

et al. 2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this study, it was aimed to determine the levels of mycotoxin contamination in poultry feeds in Konya, Afyonkarahisar, Aksaray and Nigde which constitutes 26.2% of the poultry production in Turkey. For this purpose a total of 73 readyto-consume poultry feed samples from poultry farms in four provinces were collected. A general screening in feeds was performed in terms of mycotoxin presence and contamination levels. Samples were analyzed for aflatoxin B1, ochratoxin A, zearalenone, T-2 toxin, HT-2 toxin, fumonisin and deoxynivalenol levels by LC-MS/MS multi-mycotoxin method. The levels of different mycotoxins in feeds obtained from poultry farms, the proportion of positive samples and the percentage of presence in the feeds were compared in terms of maximum residue limit (MRL). It was concluded that the feeds were found to be contaminated with low rates of mycotoxins, in terms of mycotoxin types and levels in the regions screened. However, the exceeded rates were found only 1% for aflatoxin B1 (≥ 5 ppb) in poultry feeds according to communique on undesirable substances in animal feed (RG 2014/11) while other mycotoxins were below the MRL.Keywords: Contamination, LC-MS/MS, mycotoxins, poultry feed. LC MS/MS metodu ile kanatlı yemlerinde mikotoksin varlığının araştırılmasıÖzet: Bu çalışma ile Türkiye'nin kanatlı üretiminin %26.2'sini oluşturan Konya, Afyonkarahisar, Aksaray, Niğde illerindeki kanatlı yemlerini kontamine eden mikotoksin düzeylerinin tespit edilmesi amaçlanmıştır. Bu amaçla dört ildeki kanatlı işletmelerindeki tüketime hazır kanatlı yemlerinden toplam 73 adet numune toplanmıştır. Yemlerin mikotoksin varlığı ve bulaşma seviyeleri yönünden genel bir taraması yapılmıştır. Numuneler sıvı kromotografisi/kütle kütle spektrofotometri (LC-MS/MS) cihazı ile çoklu mikotoksin metodu ile aflatoksin B1, okratoksin A, zearalenon, fumonisin, deoksinivalenol, T-2 toksin ve HT-2 toksin analiz edilmiştir. Kanatlı işletmelerinden elde edilen yemlerde farklı mikotoksin seviyeleri, pozitif örneklerin oranı ve yemlerdeki yüzde oranları, maksimum kalıntı limiti (MRL) açısından karşılaştırıldı. Taranan bölgedeki mikotoksin tip ve seviyeleri açısından, kanatlı yemlerinin mikotoksinlerle düşük düzeyde kontamine edildiği sonucuna varılmıştır. Ancak, yemlerde istenmeyen maddeler tebliği'ne göre (RG 2014/11) yasal limiti aşan %1 oranı ile sadece aflatoksin B1 (≥5ppb) hariç diğer mikotoksinlerin MRL'in altında olduğu belirlenmiştir.Anahtar sözcükler: Kanatlı yemi, kontaminasyon, LC-MS/MS, mikotoksin.

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A Review of Mycotoxins in Food and Feed Products in Portugal and Estimation of Probable Daily Intakes

Cited by 124 publications

References 84 publications

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

Visual and microplate detection of aflatoxin B2 based on NaCl-induced aggregation of aptamer-modified gold nanoparticles

Investigation of mycotoxin residues in poultry feeds by LC MS/MS method

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