A safeguard measure of endemic and endangered plant species: cryostorage of Dianthus taxa

Halmágyi, Adela; Coste, Ana; Jarda, Liliana; Butiuc-Keul, Anca; Holobiuc, Irina; Cristea, Vasile

doi:10.1007/s10531-020-02032-3

Cited by 7 publications

(5 citation statements)

References 51 publications

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“…parviflorus. The explants used for cryostorage in liquid nitrogen by the droplet-vitrification technique were shoot tips and axillary buds from plants micropropagated for two years [116] (Table 5).…”

Section: Cryopreservationmentioning

confidence: 99%

“…While several endemic and threatened plant species worldwide have been successfully cryopreserved with over 50% survival or regrowth rates [105,108], cryopreservation protocols developed at the national level have led to higher survival and regeneration rates in the targeted species (a regeneration rate of 73.3% for the subendemic taxon D. nardiformis and a survival rate of 83.3% for the endemic taxon D. henteri) [116]. This could be explained by the different types of explants used, pretreatment conditions, cryopreservation procedures, and post-thaw treatment applied [102].…”

Section: Cryopreservationmentioning

confidence: 99%

“…Regarding the number of shoots per explant after cryostorage, it varied between 3 for D. glacialis subsp. gelidus and D. henteri, and 5.6 for D. nardiformis [116] (Table 5). MS-Murashige and Skoog medium [56]; Vit B 5 -Gamborg vitamins [57]; PEG-polyethylene glycol.…”

Section: Cryopreservationmentioning

confidence: 99%

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Overview of the Success of In Vitro Culture for Ex Situ Conservation and Sustainable Utilization of Endemic and Subendemic Native Plants of Romania

Radomir¹,

Stan²,

Florea³

et al. 2023

Sustainability

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Romania has a relatively high diversity of plant species, including 3829 vascular and 979 non-vascular spontaneous plant taxa (species and subspecies). Due to uncontrolled harvesting as well as other causes, including climate change and ecological collapse, the speed of species extinction and the narrowing of the genetic base of plant resources has been reported as a critical issue. Therefore, the national Red List of Romanian flora includes 1453 threatened taxa, of which 95 are endemic and 90 subendemic. Many of these have high ornamental, medicinal–cosmetic, and/or aromatic properties. The high extinction risk of these valuable plants has stimulated both the reconsideration of their vital importance as genetic resources and interest in finding effective methods for conservation. Cultivating these phytogenetic resources in a human-controlled environment is of high importance for effective ex situ conservation, which can further serve sustainable exploitation needs and may facilitate in situ conservation actions. In vitro culture is a powerful tool for producing elite plants for cultivation for different purposes. This review summarizes the current knowledge on in vitro multiplication of 22 endemic and subendemic native plants of Romania, examining the materials used, the treatments applied, and the results obtained in each stage of the micropropagation protocol (culture initiation, proliferation, rooting, and acclimatization). The findings from the reviewed studies are presented in a comparative way, and the potential of plant tissue culture in conservation and sustainable exploitation of these Romanian species is outlined.

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Section: Cryopreservationmentioning

confidence: 99%

Section: Cryopreservationmentioning

confidence: 99%

See 1 more Smart Citation

Overview of the Success of In Vitro Culture for Ex Situ Conservation and Sustainable Utilization of Endemic and Subendemic Native Plants of Romania

Radomir¹,

Stan²,

Florea³

et al. 2023

Sustainability

View full text Add to dashboard Cite

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“…Collecting and maintaining endangered plants in tissue culture or cryopreservation to support the species’ survival and reduce its extinction risk can be a valuable alternative [ 22 , 23 , 24 , 25 ]. However, these solutions are only feasible if the practitioner can restore a rooted plant from tissue culture or cryopreservation that is then capable of being transplanted into the wild [ 26 , 27 ]; hence, the importance of developing successful protocols arises. Micropropagated plants can present in vitro generated variations, known as somaclonal variation [ 22 , 23 ]; these variations deserve to be monitored, as they can be an advantage for the conservation of wild plants with low genetic variability [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Gianguzzi

Barone

Gristina

et al. 2023

Plants

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The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L−1), Naphthaleneacetic Acid (NAA) (1 mg L−1), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L−1) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L−1) and NAA (1 mg L−1). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L−1) and NAA (0.25, 0.50, and 1 mg L−1). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L−1 of BAP and 1 mg L−1 of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L−1. These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.

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“…There are two prevailing approaches to preserving plant genetic resources: in situ conservation, which involves maintaining the plants within their natural habitat, and ex situ conservation, which entails preserving plant species outside of their natural habitat, typically in gene banks or botanical gardens. (O'Donnell and Sharrock, 2018;Halmagyi et al, 2020). Conservation of the plant species and fauna, could have an important great impact on other species, regardless of their biological importance (Sămărghiţan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Propagation of Transylvanian Endemic and Endangered Plant Species

Lihăt,

Szekely-Varga,

Csergő

et al. 2023

CTNS

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Climate change has already caused irreversible stock changes in species distributions in many parts of the world. Species are lost and disrupted because they are highly dependent on changes in temperature. The aim of the experiment is to develop cultivation techniques for wild perennial plants of ornamental value that are rare, protected or highly endangered, thus helping to maintain these plant populations while increasing the number of ornamental plants grown. As plant material we have collected the seeds of Achillea ptarmica, Achillea impatiens, Achillea x girgioensis, Allium obliquum, Silene zawadskii, Dianthus petraeus ssp. orbelicus, and Centaurea triumfettii in two different years. The collected seeds were sown in spring and autumn, alongside these the germination was determined also in Petri dishes. From our results could be determined that at the Caryophyllaceae family both genus seeds germinated reasonably good, in the case of the plants belonging to the Asteraceae family the seeds obtained different results, furthermore at the A. obliquum the germination was low. The present experiment offers the possibilities to multiply these endangered plant species, and to improve the impoverished genetic stock.

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A safeguard measure of endemic and endangered plant species: cryostorage of Dianthus taxa

Cited by 7 publications

References 51 publications

Overview of the Success of In Vitro Culture for Ex Situ Conservation and Sustainable Utilization of Endemic and Subendemic Native Plants of Romania

Overview of the Success of In Vitro Culture for Ex Situ Conservation and Sustainable Utilization of Endemic and Subendemic Native Plants of Romania

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Propagation of Transylvanian Endemic and Endangered Plant Species

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