A scanning electron microscope study of healthy pecan tissues showing the presence or absence of internal fungal contamination

Knox, Cheryl A. P.; Smith, Roberta H.

doi:10.1007/bf02619193

Cited by 6 publications

(1 citation statement)

References 6 publications

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“…Microbial contamination of plant tissue cultures (Knox and Smith, 1980;Tanaka et al, 1983) is usually caused by fungi or bacteria (Deberg, 1987;Knauss and Millar, 1978;Leifert and Waites, 1990;Long et al, 1988;Phillips et al, 1981;Young et al, 1984) that exist on or in the tissue of the original explant and are not destroyed by disinfestation. In the tissue culture environment, these microorganisms outgrow the explant tissues and cause culture loss (De Fossard, 1976;Herman, 1990).…”

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confidence: 99%

City Water Can Contaminate Tissue Culture Stock Plants

Seabrook¹,

Farrell²

1993

HortSci

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Stock plants of `Shepody' and `Yukon Gold' potato (Solarium tuberosum L.) were grown in a greenhouse and irrigated with city water. Contamination rate of stem explant tissue cultures excised from these stock plants was 50% to 100%. A comparison of the microorganisms isolated from the contaminated cultures and from 0.22-μm filter disks through which 20 liters of city water had passed revealed the presence of similar bacterial floras. Five genera of bacteria (Listerium spp., Corynebacterium spp., Enterobacter spp., Pasteurella spp., and Actinobacillus spp.) were isolated from contaminated cultures and cultured filter disks. Watering greenhouse-grown stock plants with filtered city water decreased contamination of stem explant cultures 30% to 50%. Installing an ultraviolet light water-sterilizing unit at the greenhouse inlet point effectively reduced contamination.

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mentioning

confidence: 99%

City Water Can Contaminate Tissue Culture Stock Plants

Seabrook¹,

Farrell²

1993

HortSci

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Tissue Culture of Temperate Fruit and Nut Trees

Hutchinson¹,

Zimmerman²

1987

Horticultural Reviews

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Establishing axenic cultures from mature pecan embryo explants on media with low water availability

Obeidy

Smith

1990

Plant Cell Reports

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Endophytic fungi associated with mature pecan (Carya illinoensis (Wangenh.) C. Koch) nuts prevented successful, contaminant-free in vitro culture of embryo expiants, even after rigorous surface disinfestation of the nuts and careful aseptic shelling. Disinfestation with sodium hypochlorite after shell removal was also unsuccessful, because even dilute concentrations which were ineffective against the fungal contaminants prevented subsequent growth from the embryo. Explanting media with low water availability which would not sustain growth of fungal contaminants, but supported growth from mature pecan embryos, were developed as an alternative disinfestation method. The explanting media were supplemented with 0.9-1.5% agar, and other media components were selectively omitted to test their influence on water availability and fungal growth. Disinfestation of up to 65% of the cultures was accomplished, depending on the medium formulation, compared to 100% loss to contamination on control medium (0.5% agar). A complete medium (containing sucrose, salts, vitamins, 18 μM BAP, and 5 μM IBA) with 1.5% agar provided control of contamination, and encouraged subsequent regeneration from the embryo expiants, which remained free of contaminant growth through subsequent subcultures.

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A scanning electron microscope study of healthy pecan tissues showing the presence or absence of internal fungal contamination

Cited by 6 publications

References 6 publications

City Water Can Contaminate Tissue Culture Stock Plants

City Water Can Contaminate Tissue Culture Stock Plants

Tissue Culture of Temperate Fruit and Nut Trees

Establishing axenic cultures from mature pecan embryo explants on media with low water availability

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