City Water Can Contaminate Tissue Culture Stock Plants

Seabrook, Janet E. A.; Farrell, Gerald

doi:10.21273/hortsci.28.6.628

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…Reducing relative humidity during the pregrowth period can improve the outcome of infection. Seabrook and Farrell (1993) found that irrigating donor plants with filtered water, rather than city water, decreased bacterial contamination. They observed that watering greenhousegrown Solanum tuberosum L. stock plants with filtered city water decreased contamination of explant cultures by 30 to 50%.…”

Section: Factors Affecting Disinfection Efficiencymentioning

confidence: 99%

“…They observed that watering greenhousegrown Solanum tuberosum L. stock plants with filtered city water decreased contamination of explant cultures by 30 to 50%. Installing an ultraviolet light water-disinfecting equipment at the glasshouse inlet point can successfully reduce contamination (Seabrook, Farrell 1993).…”

Section: Factors Affecting Disinfection Efficiencymentioning

confidence: 99%

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Disinfection of explants for saffron (Crocus sativus) tissue culture

Silva¹,

Kulus²,

Zhang³

et al. 2016

EEB

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The choice of explant and its preparation are most likely one of the most important biotic factors that determine the success of a tissue culture protocol when the explant is derived from ex vitro organs. This review assesses the disinfection protocols available for saffron (Crocus sativus L.; Iridaceae) explants in an attempt to ascertain the most suitable set of parameters that could ensure successful tissue culture in subsequent treatments. From a methodological perspective, two explant types are most commonly adopted in saffron biotechnology, namely the use of fresh field-grown shoots, or dormant corms. The latter is more recommended to minimize contamination and to allow the use of more aggressive disinfection treatments.

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Section: Factors Affecting Disinfection Efficiencymentioning

confidence: 99%

Section: Factors Affecting Disinfection Efficiencymentioning

confidence: 99%

Disinfection of explants for saffron (Crocus sativus) tissue culture

Silva¹,

Kulus²,

Zhang³

et al. 2016

EEB

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“…e sources of contamination of in vitro cultures are diverse. Among other things, explants collected from field-grown plants acquire the contaminants from soil [42,43] or water [44]. It becomes customary to devise procedures to clean the explants off microbial contaminants.…”

Section: E Efficacy Of Antibiotics In Controlling Bacterial Contaminamentioning

confidence: 99%

Assessing the Efficacy of Broad-Spectrum Antibiotics in Controlling Bacterial Contamination in the In Vitro Micropropagation of Ginger (Zingiber officinale Rosc)

Tewelde

Patharajan

Teka

et al. 2020

The Scientific World Journal

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Ginger (Zingiber officinale Rosc) (Zingiberaceae) is a livelihood and commercial crop in Ethiopia. But, the availability of clean and healthy planting materials has become a problem due to wilt disease, caused by Ralstonia solanacearum Biovar 3 Race 4. This problem obliged growers to seek for tens of millions of vigorous and disease-free planting materials very quickly via in vitro micropropagation of shoot tip explants. For this purpose, protocols of sterilizing shoot tip explants and controlling bacterial contamination of one Ethiopian ginger cultivar called Deribo were tested. Hence, this article reports the finding of a study that aimed at testing the (a) effectiveness of three sterilization agents, namely, 0.25% w/v RBK (composed of ridomile, bayleton, and kocide at 1 : 1 : 1 ratio), 0.50% v/v NaOCl, and 70% v/v ethanol at three different treatment times in combination with 0.25% HgCl2; (b) efficacy of four broad-spectrum antibiotics and their combinations in controlling bacterial contaminants of ginger shoot tip explants and in vitro micropropagation media; and (c) effects of the antibiotics on the shooting performances of the explants of the cultivar. A 0.50% v/v NaOCl at exposure time of 20 min followed by 0.25% HgCl2 has resulted in 80% contamination-free and 70% live explants after three weeks of incubation. Likewise, cefotaxime at 50, 100, and 200 mg/L and cefotaxime plus streptomycin at 25, 50, and 100 mg/L yielded 87 to 93% contamination-free microshoots after three weeks of culturing. The number of explants killed by the antibiotics increased with increasing the concentration of the antibiotics. Cefotaxime at 50 mg/L and cefotaxime plus streptomycin at 25 mg/L yielded significantly highest mean microshoots per explant (7.10 ± 0.36 and 7.51 ± 0.27, respectively) and mean shoot length (4.2 ± 0.26 and 3.56 ± 0.17 cm, respectively). Some of the microshoots showed some yellowing. But, they turned green and grew normal after subcultured into fresh, antibiotics-free culture media. These findings are important foundations towards developing more optimized protocols of sterilizing explants and controlling bacterial contaminants for large-scale in vitro micropropagation of the Deribo ginger cultivar.

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