A selective colorimetric sensing strategy for cysteine based on an indicator-displacement mechanism

Zhang, Xue; Xiong, Lulu; Peng, Hao; Rao, Honghong; Liu, Xiuhui; Lu, Xiaoquan

doi:10.1039/c7nj03887a

Cited by 14 publications

(4 citation statements)

References 57 publications

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“…L-cysteine is a biologically significant thiol, the discrepancies in whose levels can lead to decolorization of hair, skin and lung diseases, psoriasis, etc. [174][175][176] A recent study showed the synthesis of a nanocomposite of MoS 2 quantum dots and Ag NPs, which were employed for a colorimetric assay of cysteine [101] (Figure 8c). However, they reported a LOD of 0.82 µM, which was much higher than another nanocomposite-based cysteine detection using DNA functionalized Ag-Pt nanoclusters.…”

Section: Detection Of Natural Biomarkersmentioning

confidence: 99%

Recent Advances in Silver nanozymes: Concept, Mechanism, and Applications in Detection

Mishra

Abdal‐hay

Rather

et al. 2022

Adv Materials Inter

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physicochemical properties that are entirely different from those of their bulk counterparts. They have been exploited vigorously for numerous applications, including in medical and nonmedical fields. However, one must not realize it to be a novel technology, as its roots can be traced centuries ago. For instance, copper and silver (Ag) NPs can be dated back to the 9 th century, when they were used to shine the ceramic artifacts. [1] One of the earlier and renowned examples is the Lycurgus cup, made by the Romans in the 4 th century using Au-Ag alloy NPs to produce greenish-red color. [2] Even though the use of NPs could be found in many such old works of literature; their real potential was not completely understood until 1959, when Richard Feynman delivered the renowned lecture "There's Plenty of Room at the Bottom: An Invitation to Enter a New Field of Physics. [3] This brought in a stream of exceptional studies, and researchers were able to assign quantum size effects as the reason for a change of properties when moving from bulk to nano regime. As things became more and more precise, nanomaterials began to find their extensive role in varied applications. One of the significant applications was their role as enzyme mimetics due to their excellent catalytic properties.The metabolic reactions in our body are catalyzed by the protein molecules called enzymes. It was James B Sumner who, in 1926, established the first enzyme, urease, to be a protein, which won him a Nobel Prize for this discovery in 1946. [4] All the enzymes have been considered protein molecules ever since. However, due to their high production cost and rapid denaturation under variable pH and temperature settings, they have limited their industrial applications. [5] Therefore, a need was felt to develop enzyme mimicking materials, more precisely termed "artificial enzymes" by Robert Breslow. [6] These catalytically active molecules can be anything from polymers, cyclodextrins to supramolecules and dendrimers, showing enzyme-like activity. [7][8][9][10] Recently, the discovery of dismutaselike activity of fullerene derivatives [11,12] and peroxidase-mimicking catalysis of Fe 3 O 4 NPs [13,14] enunciated the promising career of NPs in the field of biomimetics. The importance and broad prospect of nanozymes can be understood in Figure 1. This shows the increasing number of publications each year on nanozymes. These nanozymes have been majorly used Natural enzymes accelerate substrate-specific reactions making them of exquisite interest for various applications. However, their appeal is lost in a practical setting due to low stability and high preparation expenses. This is the primary reason behind the flourishing in the research of nanozyme, i.e., artificial enzymes, in the past decade. Their unique physicochemical properties make them a promising candidate for various applications, especially in diagnostics and detection. They possess an enzyme-like activity and can overcome the drawbacks of natural protein-based enzymes. Nanozymes are nanomateri...

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Section: Detection Of Natural Biomarkersmentioning

confidence: 99%

Recent Advances in Silver nanozymes: Concept, Mechanism, and Applications in Detection

Mishra

Abdal‐hay

Rather

et al. 2022

Adv Materials Inter

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“…39,48 The detection of L-cysteine is important as its deficiency can cause anomalous cell growth, skin lesions, lethargy, hair depigmentation, liver damage, and malabsorption syndrome, 39 while high levels of L-cysteine can be neurotoxic and cause cardiovascular diseases. 49 Our previous work employing other NZs for L-cysteine sensing showed that the catalytic activity of the NZs was lost after their interaction with L-cysteine. In-depth analysis revealed that the mechanism of the interaction of L-cysteine with the NZ can be different depending on the NZ used.…”

Section: Introductionmentioning

confidence: 99%

“…In the context of catalysis, enzyme-mimicking catalytic nanoparticles or nanozymes (NZs) have gained remarkable interest with wide-spread applicability across sensing, bioimaging, environmental clean-up, and pro-drug therapy. − Given that the composition of the nanoparticle can influence the catalytic efficiency, − a suite of nanoparticles including metals, metal oxides, metal sulfides, etc ., have been reported for wide-ranging enzyme-mimic activities. , Of these, metal sulfides have been suggested to be superior catalyst candidates as the M–S bonds are weaker than the M–O bonds in the corresponding metal oxides . Further, NZ-based sensors have gained attention in analytical science as they allow visual detection of the sensing event. , Such sensors have been used for the detection of several chemical and biological molecules including l -cysteine, a thiol containing semi-essential amino acid found in a number of proteins. , The detection of l -cysteine is important as its deficiency can cause anomalous cell growth, skin lesions, lethargy, hair depigmentation, liver damage, and malabsorption syndrome, while high levels of l -cysteine can be neurotoxic and cause cardiovascular diseases …”

Section: Introductionmentioning

confidence: 99%

Cobalt Sulfide Nanosheets as Peroxidase Mimics for Colorimetric Detection of l-Cysteine

Hashmi

Singh

Weerathunge

et al. 2021

ACS Appl. Nano Mater.

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The rich chemical, physical, electronic, and surface properties of two-dimensional (2D) materials have witnessed an explosion of research leading to enrichment in the 2D family of materials. Cobalt sulfide (Co x S y ), a transition metal chalcogenide, has attracted significant interest as its different stoichiometric and non-stoichiometric phases offer diverse properties. To date, the Co3S4 phase has seen limited developments with recent reports suggesting good catalytic properties of this material. In the current study, we show the facile synthesis of phase pure 2D Co3S4 nanosheets using a hydrothermal approach and demonstrate the importance of the chemical reactivity of the surface atoms in achieving target selectivity during sensing. First, the catalytic activity of Co3S4 nanosheets was observed to follow Michaelis–Menten kinetics, suggesting that the nanosheets mimic the catalytic activity of the natural peroxidase enzymes. The presence of dangling sulfur atoms on the surface of the Co3S4 nanosheets allowed them to specifically interact with l-cysteine amino acid that resulted in the temporary loss of its catalytic activity. This interaction allowed us to develop a “turn-off” colorimetric sensor to detect l-cysteine even in complex mixtures of amino acids and other sulfur containing compounds. In-depth understanding of the sensor mechanism revealed a reversible competition between l-cysteine and peroxidase substrates for binding to the Co3S4 nanosheets. Due to the higher affinity of l-cysteine to the nanosheets, it first binds to the Co3S4 nanosheets blocking its nanozyme activity. Subsequently, the outstanding catalytic activity of 2D Co3S4 nanosheets assists in the oxidation of l-cysteine, and as oxidized products leave the nanozyme surface, its catalytic activity resumes. The findings of our study are likely to instigate further research into the exploration of the chemical reactivity of 2D surfaces for diverse applications.

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“…This method relies on the naked eye to read the concentration of various biomolecules through different intensities of color, allowing the field detection of the analyte without any complex instruments. 24–27 It is a mature and economical method for the rapid and intuitive determination of various biomolecules at trace levels in real samples and has made remarkable progress.…”

Section: Introductionmentioning

confidence: 99%

Fe-Doped polydopamine nanoparticles with peroxidase-mimicking activity for the detection of hypoxanthine related to meat freshness

Zhang

Gao

et al. 2022

Analyst

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A selective colorimetric sensing strategy for cysteine based on an indicator-displacement mechanism

Abstract: Rapid determination of cysteine in aqueous solution is important for the diagnosis and treatment of some diseases.

Cited by 14 publications

References 57 publications

Recent Advances in Silver nanozymes: Concept, Mechanism, and Applications in Detection

Recent Advances in Silver nanozymes: Concept, Mechanism, and Applications in Detection

Cobalt Sulfide Nanosheets as Peroxidase Mimics for Colorimetric Detection of l-Cysteine

Fe-Doped polydopamine nanoparticles with peroxidase-mimicking activity for the detection of hypoxanthine related to meat freshness

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