A self-referencing biosensor for real-time monitoring of physiological ATP transport in plant systems

Vanegas, Diana; Clark, Greg; Cannon, Ashley E.; Roux, Stanley J.; Chaturvedi, P.; McLamore, Eric S.

doi:10.1016/j.bios.2015.05.027

Cited by 29 publications

(20 citation statements)

References 44 publications

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“…ATP is prevalent in the apoplast of various plant species (Kim et al, 2006 ) and can be released through secretory vesicles (Kim et al, 2006 ; Wu et al, 2008 ) or plasma membrane (PM) injury (Song et al, 2006 ). Various stimuli, including touch (Jeter et al, 2004 ; Weerasinghe et al, 2009 ; Vanegas et al, 2015 ), osmotic stress (Jeter et al, 2004 ), chitin mixture (Kim et al, 2006 ), polysaccharide elicitors (Wu et al, 2008 ), and hypertonic stress (Kim et al, 2009 ), have been shown to induce ATP release.…”

Section: Introductionmentioning

confidence: 99%

Heterotrimeric G Protein-Regulated Ca2+ Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP

et al. 2017

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Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots responded similarly to ADP and bz-ATP but did not respond to AMP and GTP. The eATP avoidance response was reduced in loss-of-function mutants of heterotrimeric G protein α subunit (Gα) (gpa1-1 and gpa1-2) and enhanced in Gα-over-expression (OE) lines (wGα and cGα). Ethylenebis(oxyethylenenitrilo) tetraacetic acid (EGTA) and Gd3+ remarkably suppressed eATP-induced root bending. ATP-stimulated Ca2+ influx was impaired in Gα null mutants and increased in its OE lines. DR5-GFP and PIN2 were asymmetrically distributed in ATP-stimulated root tips, this effect was strongly suppressed by EGTA and diminished in Gα null mutants. In addition, some eATP-induced genes' expression was also impaired in Gα null mutants. Based on these results, we propose that heterotrimeric Gα-regulated Ca2+ influx and PIN2 distribution may be key signaling events in eATP sensing and avoidance response in Arabidopsis thaliana roots.

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Section: Introductionmentioning

confidence: 99%

Heterotrimeric G Protein-Regulated Ca2+ Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP

et al. 2017

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“…The actual [ATP] at the cell surface might be higher than the value in the sampled bulk medium (Weerasinghe et al, 2009 Vanegas et al (2015) detected an increase in the [eATP] to 3.4 mM after corn (Zea mays) roots were wounded. Using a cellulose-binding domainluciferase reporter, eATP hotspots have been found in elongating root cells (Kim et al, 2006).…”

Section: Peapy2 Modulated Vesicular Trafficking and Eatp Levels Undermentioning

confidence: 99%

Populus euphratica APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants

et al. 2015

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Apyrase and extracellular ATP play crucial roles in mediating plant growth and defense responses. In the cold-tolerant poplar, Populus euphratica, low temperatures up-regulate APYRASE2 (PeAPY2) expression in callus cells. We investigated the biochemical characteristics of PeAPY2 and its role in cold tolerance. We found that PeAPY2 predominantly localized to the plasma membrane, but punctate signals also appeared in the endoplasmic reticulum and Golgi apparatus. PeAPY2 exhibited broad substrate specificity, but it most efficiently hydrolyzed purine nucleotides, particularly ATP. PeAPY2 preferred Mg 2+ as a cofactor, and it was insensitive to various, specific ATPase inhibitors. When PeAPY2 was ectopically expressed in Arabidopsis (Arabidopsis thaliana), cold tolerance was enhanced, based on root growth measurements and survival rates. Moreover, under cold stress, PeAPY2-transgenic plants maintained plasma membrane integrity and showed reduced cold-elicited electrolyte leakage compared with wild-type plants. These responses probably resulted from efficient plasma membrane repair via vesicular trafficking. Indeed, transgenic plants showed accelerated endocytosis and exocytosis during cold stress and recovery. We found that low doses of extracellular ATP accelerated vesicular trafficking, but high extracellular ATP inhibited trafficking and reduced cell viability. Cold stress caused significant increases in root medium extracellular ATP. However, under these conditions, PeAPY2-transgenic lines showed greater control of extracellular ATP levels than wild-type plants. We conclude that Arabidopsis plants that overexpressed PeAPY2 could increase membrane repair by accelerating vesicular trafficking and hydrolyzing extracellular ATP to avoid excessive, cold-elicited ATP accumulation in the root medium and, thus, reduced ATP-induced inhibition of vesicular trafficking.

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“…With various structures and different test methods, the detection range of certain kind small molecules is quite wide (from around 10 -11 M to 1 M). Besides the molecules listed in the table, other small biomolecules such as quercetin ), isoquercitrin, baicalin (Liu et al 2014d), cholesterol (Agnihotri et al 2015) and ATP (Vanegas et al 2015) have also been detected by electrochemical biosensors in recent years.…”

Section: )mentioning

confidence: 98%

Synthesis of graphene and related two-dimensional materials for bioelectronics devices

Zhang

Liu

Wang

et al. 2017

Biosensors and Bioelectronics

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A self-referencing biosensor for real-time monitoring of physiological ATP transport in plant systems

Cited by 29 publications

References 44 publications

Heterotrimeric G Protein-Regulated Ca2+ Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP

Heterotrimeric G Protein-Regulated Ca2+ Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP

Populus euphratica APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants

Synthesis of graphene and related two-dimensional materials for bioelectronics devices

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