A sensitive and selective label-free DNAzyme-based sensor for lead ions by using a conjugated polymer

Xia, Chen; Guan, Hongliang; He, Zhike; Zhou, Xiaodong; Hu, Jiming

doi:10.1039/c2ay25249j

Cited by 29 publications

(33 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…121–128 A number of such studies have utilized DNA intercalating dyes that exhibit distinct fluorescence characteristics when bound with double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA) regions. 122,124–126,128 For example, Jiang and coworkers coupled the cleavage of a DNA substrate by a Pb 2+ -dependent DNAzyme in the presence of Pb 2+ with quantitative polymerase chain reaction, and subsequently measured the fluorescence of SYBR Green I upon its binding with the PCR products to detect the concentration of Pb 2+ at a high sensitivity. 122 Using graphene as the quencher for the DNA-binding GelRed dye, a label-free Cu 2+ sensor was developed based on a Cu 2+ -dependent DNAzyme.…”

Section: Metal Ion-dependent Dnazymes For Metal Ion Recognitionmentioning

confidence: 99%

DNA as Sensors and Imaging Agents for Metal Ions

Xiang

Lu²

2013

Inorg. Chem.

142

104

View full text Add to dashboard Cite

Increasing interests in detecting metal ions in many chemical and biomedical fields have created demands for developing sensors and imaging agents for metal ions with high sensitivity and selectivity. This review covers recent progress in DNA-based sensors and imaging agents for metal ions. Through both combinatorial selection and rational design, a number of metal ion-dependent DNAzymes and metal ion-binding DNA structures that can selectively recognize specific metal ions have been obtained. By attaching these DNA molecules with signal reporters such as fluorophores, chromophores, electrochemical tags, and Raman tags, a number of DNA-based sensors for both diamagnetic and paramagnetic metal ions have been developed for fluorescent, colorimetric, electrochemical, and surface Raman detections. These sensors are highly sensitive (with detection limit down to 11 ppt) and selective (with selectivity up to millions-fold) toward specific metal ions. In addition, through further development to simplify the operation, such as the use of “dipstick tests”, portable fluorometers, computer-readable discs, and widely available glucose meters, these sensors have been applied for on-site and real-time environmental monitoring and point-of-care medical diagnostics. The use of these sensors for in situ cellular imaging has also been reported. The generality of the combinatorial selection to obtain DNAzymes for almost any metal ion in any oxidation state, and the ease of modification of the DNA with different signal reporters make DNA an emerging and promising class of molecules for metal ion sensing and imaging in many fields of applications.

show abstract

Section: Metal Ion-dependent Dnazymes For Metal Ion Recognitionmentioning

confidence: 99%

DNA as Sensors and Imaging Agents for Metal Ions

Xiang

Lu²

2013

Inorg. Chem.

142

104

View full text Add to dashboard Cite

show abstract

“…Until now, considerable efforts have been devoted to the assay of Pb 2+ utilizing different receptors, including DNAzymes (Chen et al, 2012;Wang et al, 2008), proteins (Lin and Chung, 2008;Shete and Benson, 2008), oligonucleotides (Li et al, 2011;Lu et al, 2013), polymers (Lee et al, 2011) and nanomaterials (Aragay et al, 2011;Zheng et al, 2010). Gold nanoparticles (Au NPs) is one of the most commonly used receptors attribute to it´s unique optoelectronic properties, ease of surface functionalization and high surface-to-volume ratio.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric sensor strips for lead (II) assay utilizing nanogold probes immobilized polyamide-6/nitrocellulose nano-fibers/nets

Wang

et al. 2013

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…82 A simple “mix-and-detect” approach enables the detection of Pb 2+ within 20 minutes due to the distinguishable optical properties of PT–dsDNA and PT–ssDNA. The detection was well below the standard EPA at 0.2 ppb but this methods has a wide detection range, 2 ppb to 20 ppm, enabling diverse functional.…”

Section: Dnazymesmentioning

confidence: 99%

“…The detection was well below the standard EPA at 0.2 ppb but this methods has a wide detection range, 2 ppb to 20 ppm, enabling diverse functional. 82 This method avoids modification and separation thus making this simple, sensitive, specific, and cost-effective approach showed great potential in environmental monitoring, waste management and cellular understanding.…”

Section: Dnazymesmentioning

confidence: 99%

Continuing Issues with Lead: Recent Advances in Detection

Deibler

Basu

2012

Eur J Inorg Chem

View full text Add to dashboard Cite

In the past Pb2+ has been used in many industries, including gasoline, piping, toys, paints, and more. The use of lead has led to a natural increase of lead concentration in the environment especially in air and water. According to the U.S. CDC “no level of lead in blood is considered safe.” Exposure to very low amounts of lead can cause several health complications including developmental and neurological disorders. Over the past several years an emphasis has been placed in developing systems that can detect lead at a very low concentration. A great deal of work has been accomplished in the development of Pb2+ sensors that can not only detect but also quantify the amount and in some cases in the presence of other metal ions. Herein, we describe current regulations, mode of exposure and recent development of sensing techniques.

show abstract

A sensitive and selective label-free DNAzyme-based sensor for lead ions by using a conjugated polymer

Cited by 29 publications

References 34 publications

DNA as Sensors and Imaging Agents for Metal Ions

DNA as Sensors and Imaging Agents for Metal Ions

Colorimetric sensor strips for lead (II) assay utilizing nanogold probes immobilized polyamide-6/nitrocellulose nano-fibers/nets

Continuing Issues with Lead: Recent Advances in Detection

Contact Info

Product

Resources

About