A SERS aptasensor based on AuNPs functionalized PDMS film for selective and sensitive detection of Staphylococcus aureus

Aptasensors for Staphylococcus aureus Risk Assessment in Food

“…Under optimized experimental conditions, LOD is 13 CFU/ml. The linear range of this experiment was 4.3 × 10 to 4.3 × 10 7 CFU/ml (Zhu et al, 2021). Zhang et al (2015) used Raman molecule-modified AuNPs and aptamers as signal probes.…”

“…It is generally believed that the electromagnetic field on the metal surface can be expressively raised due to plasma excitation because the electromagnetic mechanism provides most of the enhancement ( Payton et al, 2014 ). Zhu et al (2021) assembled a S. aureus aptasensor using SERS technology based on an aptamer functionalized polydimethylsiloxane (PDMS) membrane. The aptamer was fixed on the AuNPS-PDMS membrane by Au-S.…”

Conventional and emerging strategies for the fabrication and functionalization of PDMS-based microfluidic devices

“…Zhu et al demonstrated the immobilization of AuNPs on amine treated PDMS, which were subsequently conjugated with thiol-terminal Staphylococcus aureus ( S. aureus ) aptamers in order to detect S. aureus via surface-enhanced Raman scattering (SERS). 164 Applying this surface functionalization technique to PDMS microfluidics can potentially enhance the limit of detection.…”

Recent Advances in Surface-Enhanced Raman Scattering Magnetic Plasmonic Particles for Bioapplications

“…In comparison with other methods like ELISA based on monodisperse magnetic particles (10 4 –10 5 cells mL −1 LOD [ 64 ]), PCR method (10 cells mL −1 LOD [ 65 ]), real-time potentiometric biosensors based on carbon nanotubes and aptamers, (8 × 10 2 cells mL −1 LOD [ 66 ]), the sensitivity of MPPs based on SERS is better or equal but it is simpler in procedure while other approaches require special expensive instrumentation, trained technicians, or complicated pretreatment protocol [ 18 ]. Even in comparison with Au NP-based SERS (10–13 cells mL −1 LOD [ 67 , 68 ]), the method needs a separation step of target, MPP-based SERS only need 10 s for separation based on magnetic property [ 18 ]. For aromatic dye detection, SERS is a powerful method in this field; however, many aromatic dyes have a poor affinity in interactions with SERS material that leads to the necessity of modification of SERS substrate that can detect aromatic dyes as low as 1 nM LOD [ 69 , 70 , 71 ].…”