A shared and unique peptide in the human colon, eye, and joint detected by a monoclonal antibody

Bhagat, S.; Das, Kiron M.

doi:10.1016/0016-5085(94)90066-3

Cited by 114 publications

(67 citation statements)

References 23 publications

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“…CCA-IgG inhibited the binding of 7E|2H[2 MoAb in an inhibition ELISA and in the lissue section, suggesting thai both antibodies react with the same protein [4]. The 7E|2H,;-reactive protein has since been also localized in epithelial cells ofthe skin and biliary tract [21], non-pigmented ciliary epithelial cells in the eye and chondrocytes in the joints [22]: ihe extra coionic organs that are often involved in UC. Furthermore, patients with primary sclerosing cholangiiis have circulating autoantibodies against the 7Ei2H|2-reactive protein present in the biliary epithelial cells [23].…”

Section: Discussionmentioning

confidence: 99%

Expression of a unique protein on colon cancer cells that reacts with a novel monoclonal antibody and ulcerative colitis serum

Hassan

Kanisawa

Meyers

et al. 1995

Clinical and Experimental Immunology

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SUMMARYWe earlier developed a MoAb, 7E|2H[2 (IgM isotype), against a protein present in normal colonic epithelial cells. To examine if 7E,3Hi2-reactive protein is expressed in colon cancer cells and is recognized by ulcerative colitis (UC}-associated autoantibody. we investigated several colon cancer cell lines. 7E12H12 reactivity against the cells was examined by indirect immunofluorescence assay and whole cell HLISA against six colon cancer cell lines HT-29, LoVo, COLO 205, DLD-1. LS 180 and SW 1116, A competitive ELISA was developed using 7E|.H|. MoAb and patients' serum to examine the cross-reactive antibodies in the serum. Among the six colon cancer cell lines only LS 130, DLD-! and SW 1116 reacted with 7E12H1. MoAb. while others did not. The mean (is.e.m.) inhibition of the binding or7Ei^H]2 MoAb to LS 180 cells by UC serum (n=5l) was 42±2iyo, whereas in normal subjects («=I7) it was I4±2-6%, in Crohn's disease (/T=19) it was 15-3±2-5%. in infectious diarrhoea (n^IO) it was 11%±3%. and in systemic lupus erythematosus («=10) it was 2% ±0-6%. The inhibition by the UC group was significantly (/'<0 OOI-

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Section: Discussionmentioning

confidence: 99%

Expression of a unique protein on colon cancer cells that reacts with a novel monoclonal antibody and ulcerative colitis serum

Hassan

Kanisawa

Meyers

et al. 1995

Clinical and Experimental Immunology

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“…Various strategies have been employed in an attempt to identify the underlying pathogenesis of these autoimmune eye findings. A specific peptide, 7E 12 H 12 -reactive peptide, has recently been shown to be shared by colonic epithelium and the nonpigmented epithelial layer of the ciliary processes of the eye (23). Studies including the evaluation of ocular muscles for this peptide would be informative.…”

Section: Discussionmentioning

confidence: 99%

Keeping an Eye on Crohn’s Disease: Orbital Myositis as the Presenting Symptom

Durno¹,

Ehrlich²,

Taylor³

et al. 1997

Canadian Journal of Gastroenterology

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Episodic periorbital swelling due to presumed orbital inflammation and myositis caused intermittent apparent proptosis and was the presenting symptom of ileocecal Crohn's disease (CD) in a teenage female with a family history of autoimmune disorders and CD. Orbital myositis, a very rare extraintestinal manifestation of inflammatory bowel disease (IBD), likely represents a process of impaired immunoregulation related to the underlying intestinal inflammation. This rare manifestation of IBD simulates the more commonly encountered thyroid orbitopathy (ophthalmopathy), but IBD should be considered if all thyroid tests are negative. It is important to recognize that orbital myositis may be an extraintestinal manifestation of Crohn's disease so that the diagnosis can be made and appropriate therapy commenced.

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“…Most commonly, involvement of central and peripheral joints, skin, eyes and the hepatobiliary system is seen [3][4][5][6][7]. It is most probably related to the presence of circulating antibodies against a colonic epithelial antigen that is shared among these organs [8]. The evidence of other organs involvement is smaller; however, it seems that practically any organ can be affected.…”

Section: Introductionmentioning

confidence: 90%