A short histone H3 promoter from alfalfa specifies expression in s-phase cells and meristems

Kapros, Tamás; Stefanov, I.; Magyar, Zoltán; Ocsovszky, I.; Dudits, Dénes

doi:10.1007/bf02632235

Cited by 19 publications

(31 citation statements)

References 34 publications

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“…6J) in northern analyses. This range is smaller than the factor 20 to 50, observed for histones in synchronized higher animal and plant cells in culture (Osley, 1991;Kapros et al, 1993). The degree of cell-cycle synchrony observed in our experiments (Fig.…”

Section: Radioactive Acetate Labeling Of Histonescontrasting

confidence: 38%

Histones of Chlamydomonas reinhardtii (Synthesis, Acetylation, and Methylation)

et al. 1995

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Histones of the green alga Chlamydomonas reinhardfii were prepared by a new method and fractionated by reversed-phase high-performance liquid chromatography. Acid-urea-Triton gel analysis and tritiated acetate labeling demonstrated high levels of steady-state acetylation for the single histone H3 protein, in contrast to low levels on histones H4 and H2B. Twenty percent of histone H3 is subject to dynamic acetylation with, on average, three acetylated lysine residues per protein molecule. Histone synthesis in light-dark-synchronized cultures was biphasic with pattern differences between two histone H1 variants, between two H2A variants, and between H2B and ubiquitinated H2B. Automated protein sequence analysis of histone H3 demonstrated a site-specific pattern of steady-state acetylation between 7 and 17% at five of the six amino-terminal lysines and of monomethylation between 5 and 81 O/O at five of the eight amino-terminal lysines in a pattern that may limit dynamic acetylation. An alga1 histone H 3 sequence was confirmed by protein sequencing with a single threonine as residue 28 instead of the serinez8-alaninez9 sequence, present in all other known plant and animal H 3 histones.

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Section: Radioactive Acetate Labeling Of Histonescontrasting

confidence: 38%

Histones of Chlamydomonas reinhardtii (Synthesis, Acetylation, and Methylation)

et al. 1995

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Section: Introductionmentioning

confidence: 99%

“…It was shown that the cyclAt promoter mediated an increased expression in oryzalintreated root tips (19). Promoter-reporter gene analyses in synchronized suspension cultures have only been performed for histone genes (20)(21)(22) MATERIALS AND METHODS Construction of Chimeric Genes. Construction of the cyclAt promoter-gus-3' octopine synthase gene in a pGUS1 cloning vector and a pGSV4 binary vector has been described (19).…”

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confidence: 99%

Two Arabidopsis cyclin promoters mediate distinctive transcriptional oscillation in synchronized tobacco BY-2 cells.

Shaul

Mironov

Burssens

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

169

110

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Cyclins are cell cycle regulators whose proteins oscillate dramatically during the cell cycle. Cyclin steady-state mRNA levels also fluctuate, and there are indications that both their rate of transcription and mRNA stability are under cell cycle control. Here, we demonstrate the transcriptional regulation of higher eukaryote cyclins throughout the whole cell cycle with a high temporal resolution. The promoters of two Arabidopsis cyclins, cyc3aAt and cyclAt, mediated transcriptional oscillation of the 8-glucuronidase (gus) reporter gene in stably transformed tobacco BY-2 cell lines. The rate of transcription driven by the cyc3aAt promoter was very low during G1, slowly increased during the S phase, peaked at the G2 phase and G2-to-M transition, and was down-regulated before early metaphase. In contrast, the rate of the cyclAt-related transcription increased upon exit of the S phase, peaked at the G2-to-M transition and during mitosis, and decreased upon exit from the M phase. This study indicates that transcription mechanisms that seem to be conserved among species play a significant role in regulating the mRNA abundance of the plant cyclins. Furthermore, the transcription patterns of cyc3aAt and cyclAt were coherent with their slightly higher sequence similarity to the A and B groups of animal cyclins, respectively, suggesting that they may fulfill comparable roles during the cell cycle.Cyclins are activators of specific serine/threonine protein kinases, termed CDKs, which drive progression of the eukaryotic cell cycle (reviewed in ref. 1). Based upon sequence analyses, most plant cyclins identified so far can be divided into those showing slightly higher sequence similarity to either the A or B groups of animal cyclins, but these similarities are not sufficient to exclusively assign them to either group (2, 3). Animal A-and B-type cyclins have distinct patterns of expression and fulfill different roles throughout the cell cycle (reviewed in ref. 4). As the roles of cyclins are far more understood in animals than in plants, further affiliation of a plant cyclin to a certain group of animal cyclins based on a similar expression pattern may give a clue to its function. The Arabidopsis cyclAt and cyc3aAt cyclin genes represent plant cyclins with slightly higher homology to the B and A groups of animal cyclins, respectively (2). Whole-mount in situ hybridization of Arabidopsis root tips treated with cell cycle blockers indicated that steady-state mRNA levels of cyclAt are high at early metaphase and low at early S phase, while the opposite was true for cyc3aAt (2). These data indicated that mRNA levels of cyc3aAt are increased in advance to that of cyclAt, but were not sufficient to give a complete picture of the expression pattern and transcriptional regulation of these cyclins throughout the whole cell cycle.Fluctuation in the steady-state mRNA level of a cell cycle gene may be regulated by a change in the rate of transcription, or in mRNA stability, or both. Transcriptional regulation of cell cycle genes...

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“…Preferential histone gene expression during the S phase, both in plant tissues and in cell-suspension cultures, has been demonstrated in a large number of systems (e.g. Kapros et al, 1992;Tanimoto et al, 1993;Reichheld et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Identification of Proliferation-Induced Genes in Arabidopsis thaliana (Characterization of a New Member of the Highly Evolutionarily Conserved Histone H2A.F/Z Variant Subfamily)

Callard

Mazzolini

1997

Plant Physiology

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New tissues and organs are generally initiated throughout the life of the plant from small, highly organized groups of cells called meristems (Medford, 1992). Occasionally, however, adventitious organ development occurs as a consequence of the reactivation of cell division in previously quiescent cells of differentiated tissues (Yang et al., 1994). A better understanding of how developing plants achieve such a tight control of proliferative activity in meristematic and differentiated tissues will come from the elucidation of the molecular events involved in the reentry and progression through the cell cycle in the plant cell (e.g.

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A short histone H3 promoter from alfalfa specifies expression in s-phase cells and meristems

Cited by 19 publications

References 34 publications

Histones of Chlamydomonas reinhardtii (Synthesis, Acetylation, and Methylation)

Histones of Chlamydomonas reinhardtii (Synthesis, Acetylation, and Methylation)

Two Arabidopsis cyclin promoters mediate distinctive transcriptional oscillation in synchronized tobacco BY-2 cells.

Identification of Proliferation-Induced Genes in Arabidopsis thaliana (Characterization of a New Member of the Highly Evolutionarily Conserved Histone H2A.F/Z Variant Subfamily)

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