A short review of discovery and development of fish sperm cryopreservation

Abstract

Global biodiversity, especially fish, has experienced a decline, this occurs as a result of over-exploitation, the presence of introduced fish species and climate change. This condition makes researchers look for solutions to overcome these problems by using cryopreservation techniques. The main purpose of cryopreservation is to store, maintain, and ensure the survival of genetic material, so that using cryopreservation techniques can maintain the viability and function of gamete cells both immunologically, biologically and physiologically. The success of the cryopreservation technique has made this technique widely developed in various species of living organism including fish. This article summarizes and reviews the history of the development of cryopreservation of animal species with specific focus on fish.Keywords:CryopreservationHistoryDepikEndemic species

Endemic Fish Conservation: Utilization of Cryopreservation Technology with Fructose in Red Bader Fish (Puntius Bramoides) Sperm

“…Conservation is essential to protect species-rich habitats and threatened endemic species (Arthington et al, 2016). The use of cryopreservation technology in fish has enormous value because populations can be protected from extinction due to disease, natural disasters, or overexploitation (Afriani et al, 2021). Conservation efforts to prevent the extinction of the red Bader fish are cryopreservation.…”

The effect of cryoprotectant on the viability and motility of goldfish Carassius aurtus sperm after 48 hours refrigerated

“…Blaxter (1953) was the first researcher studied the sperm preservation on herring fish Clupea harengus sperm with carbon dioxide and 15% glycerol at -79 o C, this technique was previously developed by Polge and Rowson (1952) for bull sperm. Presently, many species of freshwater and marine fish are being studied for sperm storage techniques (Afriani et al, 2021). The principle of low-temperature sperm preservation is to maintain and extend the life span of spermatozoa by reducing the metabolic rate of sperm cells during storage (Lemma, 2011).…”