A signal-amplified electrochemical immunosensor for aflatoxin B1 determination in rice

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B1in Rice

“…15 In recent years, electrochemical immunosensors have been developed for specific detection of toxic small molecules using monoclonal antibodies (MAbs). [16][17][18] In addition, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) are useful techniques for the development of immunosensors due to their high sensitivity, low cost and possibility of instrument miniaturization. 19 EIS is based on the application of a small alternating current (AC) potential as a function of time to measure the current generated.…”

Application of Modern Biosensors Methods in Ecotoxicological Monitoring of Some Toxins of Natural (Micotoxins) and Antropogenic (Pesticides) Origin. Part 1. Micotoxins.

“…Крім того, застосування електродних сен-сорів включають визначення АФМ 1 в молоці [86,87], АФВ 1 в ячмені [88], АФВ 1 в зубчасто-му рисі [89], ОТА в пшениці [90] та Т-2 токси-ну в ячмені [91]. Перевагою електрохімічних випробувань є дешеве виробництво електро-дів; недоліками -дрейф сигналу і складність регенерації антитіл.…”

Aflatoxin Measurement and Analysis

“…Finally, the binding enzyme density can be shown by current produced from the catalytic oxidation reaction of the enzyme with substrates. Many such immunoassays have been described for aflatoxins (Ammida et al, 2004;Micheli et al, 2005;Parker & Tothill, 2009;Tan et al, 2009;Vig, et al, 2009) and they all had high sensitivities (LOD 0.01-0.4 ng/mL). With a non-competitive immunoassay, the formation of the Ab-Ag complex by a simple one-step immunoreaction between the immobilized enzyme-Ab conjugate and analytes in sample solution introduced a barrier of direct electrical communication between the immobilized enzyme and the electrode surface, so local current variations could be detected by the enzyme bioelectrocatalytic oxidation reaction with substrates.…”