A simple and effective approach for the treatment of chronic wound infections caused by multiple antibiotic resistant Escherichia coli

Nagoba, Basavraj; Wadher, B.J.; Rao, Arunkumar; Kore, G.D.; Gomashe, Ashok V.; Ingle, Arun B.

doi:10.1016/j.jhin.2008.03.014

Cited by 31 publications

(26 citation statements)

References 6 publications

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“…Although there are several mechanisms for the development of resistance, mutation in the gyrA gene is the most common. 16 The high occurrence of ciprofloxacin resistance (75.9%) in our isolates is in accordance with some other reports from Asia, 15,17 but it is in contrast to a report from Vietnam. 18 As expected, the incidence of resistance to nalidixic acid, which is a simple quinolone, was higher (93.1%) and in agreement with some earlier reports.…”

Section: Discussionsupporting

confidence: 91%

“…These results are in accordance with earlier reports from Pakistan and India. 15,19 The resistance to gentamicin (86.2%) and streptomycin (72.4%) was found to be relatively high as compared with some earlier reports. 4,15,19 Tetracycline has been a widely used antimicrobial drug, but all of our isolates showed resistance to it.…”

Section: Discussionmentioning

confidence: 62%

“…It is in accordance with observations made by other researchers. 15 There were 20.7% isolates resistant to ampicillin by the disc diffusion method, but were negative for the three most common genes involved in resistance against ampicillin (TEM, bla TEM and bla OXA genes). The drug resistance in these isolates may be related to several other genes that were not included in this study.…”

Section: Discussionmentioning

confidence: 97%

“…However, the high sensitivity to cefoperazone (34.5%) is not in line with some earlier reports. 15 Fluoroquinolones are perhaps the most important synthetic antimicrobial drugs used in clinical practice. Although there are several mechanisms for the development of resistance, mutation in the gyrA gene is the most common.…”

Section: Discussionmentioning

confidence: 99%

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A profile of drug resistance genes and integrons in E. coli causing surgical wound infections in the Faisalabad region of Pakistan

et al. 2009

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Escherichia coli are one of the leading causes of infection in wounds. Emerging multiple drug resistance among E. coli poses a serious challenge to antimicrobial therapy for wounds. This study was conducted to ascertain a baseline profile of antimicrobial resistance in E. coli isolates infecting surgical wounds. A total of 64 pus samples from hospitalized patients were screened and 29 (45.3%) were found to have E. coli, which were identified biochemically and confirmed by molecular methods. Using the disc diffusion method, antimicrobial resistance was observed toward tetracycline (100%), cefradine (100%), nalidixic acid (93.1%), ampicillin (86.2%), gentamicin (86.2%), cefixime (82.8%), ceftriaxone (82.8%), aztreonam (82.8%), ciprofloxacin (75.9%), streptomycin (72.4%), cefoperazone (65.5%), chloramphenicol (58.6%) and amikacin (58.6%). In an effort to find relevant genes, 11 different genes were targeted by PCR. Among these, the mutated gyrA gene was found to be the most prevalent (82.8%), followed by the TEM (72.4%), catP (68.9%), catA1 (68.9%), tetB (62.1%), blt (58.6%), bla CTXÀMÀ15 (27.6%), bla TEM (20.7%), bla OXA (17.2%), tetA (17.2%) and aadA1 (13.8%) genes. The presence of integrons was also studied among these isolates. The prevalence of class 1 integrons was the highest (44.8%), followed by class 2 (27.6%). Three (10.3%) isolates carried both class 1 and class 2 integrons (first report from E. coli infecting wounds). The high incidence of integrons points toward their facilitation for carriage of antimicrobial resistance genes; however, in nearly 37% isolates, no integrons were detected, indicating the significance of alternative mechanisms of gene transfer. Another salient finding was that all isolates were multidrug-resistant E. coli.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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A profile of drug resistance genes and integrons in E. coli causing surgical wound infections in the Faisalabad region of Pakistan

et al. 2009

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“…Nagoba et al (2002) treated non healing ulcers in leprosy patients by citric acid. Nagoba et al (2008) employed the citric acid for the treatment of chronic wound infections caused by antibiotic resistant E. coli. A total of 34 cases of chronic wound infections yielding MAREC isolates on culture were studied.…”

Section: Ansfmentioning

confidence: 99%

Citric acid: A potential permeabilizer against multiple drug resistance enteropathogenic Escherichia coli

Dharmik

Gomashe

Wadher

2012

JANS

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Enteric diseases enter through the mouth and are usually spread by contaminated food, water or contact with contaminated vomit or feces. Enteric infection encompasses all the infections of the intestinal tract. These intestinal infections include organisms like Escherichia coli, Salmonella, Shigella, Klebsiella, Proteus etc. Out of these, E. coli are one of the common causes of enteric infection. In spite the introduction of a wide variety of antimicrobial agents against enteric diseases, life threatening infections caused by E. coli contributes to morbidity and mortality in patients. The present study was conducted to determine the antibiotic sensitivity pattern of E. coli obtained from stool samples and potentiation of antibiotic activity by citric acid against multiple drug resistant E. coli. Out of the 200 isolates of E. coli, 150 were found to be resistant to one or more antibiotics tested. 0.05% and 0.1% citric acid was found to be effective in increasing the potency of the all the antibiotics used in the study.

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Panthenol Citrate Biomaterials Accelerate Wound Healing and Restore Tissue Integrity

Wang

Duan

Keate

et al. 2023

Adv Healthcare Materials

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Impaired wound healing is a common complication for diabetic patients and effective diabetic wound management remains a clinical challenge. Furthermore, a significant problem that contributes to patient morbidity is the suboptimal quality of healed skin, which often leads to reoccurring chronic skin wounds. Herein, a novel compound and biomaterial building block, panthenol citrate (PC), is developed. It has interesting fluorescence and absorbance properties, and it is shown that PC can be used in soluble form as a wash solution and as a hydrogel dressing to address impaired wound healing in diabetes. PC exhibits antioxidant, antibacterial, anti‐inflammatory, and pro‐angiogenic properties, and promotes keratinocyte and dermal fibroblast migration and proliferation. When applied in a splinted excisional wound diabetic rodent model, PC improves re‐epithelialization, granulation tissue formation, and neovascularization. It also reduces inflammation and oxidative stress in the wound environment. Most importantly, it improves the regenerated tissue quality with enhanced mechanical strength and electrical properties. Therefore, PC could potentially improve wound care management for diabetic patients and play a beneficial role in other tissue regeneration applications.

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A simple and effective approach for the treatment of chronic wound infections caused by multiple antibiotic resistant Escherichia coli

Cited by 31 publications

References 6 publications

A profile of drug resistance genes and integrons in E. coli causing surgical wound infections in the Faisalabad region of Pakistan

A profile of drug resistance genes and integrons in E. coli causing surgical wound infections in the Faisalabad region of Pakistan

Citric acid: A potential permeabilizer against multiple drug resistance enteropathogenic Escherichia coli

Panthenol Citrate Biomaterials Accelerate Wound Healing and Restore Tissue Integrity

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