A simple and reliable method to perform biomechanical evaluation of postoperative nerve adhesions

Crosio, Alessandro; Valdatta, Luigi; Cherubino, Mario; Izzo, Matteo; Pellegatta, Igor; Pascal, Davide; Geuna, Stefano; Tos, Pierluigi

doi:10.1016/j.jneumeth.2014.06.008

Cited by 14 publications

(22 citation statements)

References 11 publications

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“…Fourteen nerves from each group were included in bio-mechanical analysis and two nerves per group underwent histological analysis. To perform our surgery on the animals, we employed a versatile, widespread and cost-effective animal model that has been described recently by our group in a previous paper [15]. In summary, the athymic adult mice were anaesthetized using a combination of 100 mg/kg of Ketamine and 15 mg/kg of Xylazine applied intraperitoneally.…”

Section: Methodsmentioning

confidence: 99%

“…In the burning group, after retraction of the nerve, we burned the muscle surface with a surgical diathermy ( Bovie Cautery pen micro tip , Bovie Medical Corporation, NY, USA) for approximately 0.8 cm along the nerve bed, to create the scar adhesion as previously described [15, 16]. …”

Section: Methodsmentioning

confidence: 99%

“…Two nerves from each group were not tested bio-mechanically but were processed for histological evaluation. Bio-mechanical evaluation was performed to measure the peak force required to pull the nerve out from the muscular bed by methods and tools described in a previous work [15]. Briefly, the tool consists of using a force applied constantly to the nerve until the traction breaks the adhesions between the nerve and the surrounding tissue.…”

Section: Methodsmentioning

confidence: 99%

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Use of human fat grafting in the prevention of perineural adherence: Experimental study in athymic mouse

et al. 2017

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Perineural adherences represent a problem after surgery involving peripheral neural system. Fat-grafting with adipose derived stem cells (ASCs) with their pro-regenerative characteristics can be important to prevent the neural damage or to facilitate the neural regeneration. Our idea was to use the fat-grafting as an anti-adherence device and test its efficacy on a postsurgical scar animal model and comparing to an antiadhesive gel. 32 athymic mice were operated under magnification, we exposed both sciatic nerves. We randomly divided all sciatic nerves into four experimental groups: burning (1), burning + carboxy-methylcellulose and poly- ethylene oxide (CMC-PEO) (2) + human adipose fat tissue (3), control group (4). Bio-mechanical evaluation was performed to measure the peak force required to pull out the nerve from the muscular bed. Results: in the CMC-PEO group the peak pull out force was 0.37 Newton. In the fat grafted group we registered a peak pull out force of 0.35 N (t Student 0.913). In burning group the force necessary to tear the nerve apart was markedly superior (0.46 N). In control group, we reported the minimal strength (0.31 N) to slide the nerve from the tissue. Histologically, in the group treated with fat-grating, a thinner scar layer was highlighted. Considering the results of this study we can support the efficacy in animal experimental model of fat graft as an anti-adherence device in peripheral nerve surgery.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Use of human fat grafting in the prevention of perineural adherence: Experimental study in athymic mouse

et al. 2017

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“…All procedures were performed in accordance with the Local Ethics Committee and the European Communities Council Directive of EU/63/2010. To perform our analysis, we used a versatile, common, and cost‐effective animal model described recently …”

Section: Methodsmentioning

confidence: 99%

“…In this study, we tested the efficacy of Dynavisc® (FzioMed, Inc), a CMC‐PEO gel for reduction of perineural scar tissue formation in a mouse model by evaluating the peak pull out force and histological aspects of the muscle–nerve interface, before and after gel application on an injured sciatic nerve model following methods described previously …”

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confidence: 99%

Efficacy of anti-adhesion gel of carboxymethylcellulose with polyethylene oxide on peripheral nerve: Experimental results on a mouse model

et al. 2015

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Comparisons of fascio‐cutaneous anterolateral thigh and sandwich fascial ALT free flap in the distal extremity reconstruction

et al. 2019

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Introduction:The anterolateral thigh (ALT) flap is considered a workhorse reconstructive option; however, it is encumbered by its bulkiness that can result in poor final outcome and need for revision surgery. The aim of the present study was to compare the standard cutaneous ALT free flap and sandwich fascial ALT (SALT) free flap, raised harvesting between the Scarpa's fascia and the crural fascia, for distal extremity soft tissue reconstruction, including pre-intra-and post-operative considerations and outcomes.Patients and methods: A retrospective review of medical records from 2013 to 2018 of 24 patients who underwent distal extremity reconstruction with standard fasciocutaneous ALT flap (13 patients) and SALT flap (11 patients) was performed. The mean defect dimensions were 12 × 6.5 cm in group 1 and 12 × 6 in group 2. Surgical outcomes and quality of life were assessed (through the upper extremity functional scale and the lower extremity functional scale questionnaires. Results:The mean flap dimensions were 13.1 × 7.1 in group 1 and 14.1 × 7.8 in group 2, the overall flap success rate was 100% (one microvascular venous thrombosis occurred in group 1), no statistically significant difference was recorded regarding microvascular thrombosis (one patient in group p = .369) and infections (one patient in group 1, p = .36) while a statistically significant difference was presence regarding the number of secondary/debulking procedures (6 patients vs. 0) (p = .0076) and the quality of life perception showed an overall better perception in group 2 with statistical significant difference (p = .03). Conclusion:The SALT flap represents a valid option, as showed through our preliminary data, when a thin and robust reconstruction is required when dealing with distal extremities soft tissue reconstruction.

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A simple and reliable method to perform biomechanical evaluation of postoperative nerve adhesions

Abstract: Background: Perineural fibrotic adhesions are among the major complications of

Cited by 14 publications

References 11 publications

Use of human fat grafting in the prevention of perineural adherence: Experimental study in athymic mouse

Use of human fat grafting in the prevention of perineural adherence: Experimental study in athymic mouse

Efficacy of anti-adhesion gel of carboxymethylcellulose with polyethylene oxide on peripheral nerve: Experimental results on a mouse model

Comparisons of fascio‐cutaneous anterolateral thigh and sandwich fascial ALT free flap in the distal extremity reconstruction

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