Efficacy of anti-adhesion gel of carboxymethylcellulose with polyethylene oxide on peripheral nerve: Experimental results on a mouse model

Abstract

CMC-PEO gel can reduce perineural scar tissue. In histological section, scar tissue was present in both groups, but in the Burned+gel group a gliding surface was identified between scar and nerve.

Use of human fat grafting in the prevention of perineural adherence: Experimental study in athymic mouse

“…The major drawback of these products is the additional cost. Our animal model is an easy and reproducible model to study the perineural adherences and the interaction with anti-adherence devices [18,8]. This study is focused on the validity of the human fat grafting in the prevention of the perineural adherences.…”

“…The sample that was immediately conserved in ice and applied in the experimental animal in the next 90 minutes. The athymic mice were required to prevent a xenogeneic reaction to the injection of human fat but develop enough scar tissue around the nerve as demonstrated in our previous study [8]. In our other parallel study about ASCs properties [18] we analyzed the sub populations of lipoaspirate used in this study, showing a homogenous population of adipose-derived stem cells (ASCs) characterized by the presence of CD44, CD90, CD105 and HLA class I markers and the absence of the CD45 marker.…”

“…Distally the nerve was dissected at the trifurcation at the knee, then the sciatic nerve has been cut just above the trifurcation. This method had been studied and described in a previous work cited in references section [8][20] A schematic view of the extraction tool is presented in Fig 2. The force is reported in Newtons (N).…”

“…Few reports are available in the literature regarding the efficacy of these types of barriers in the peripheral neural system. A previous study conducted by our team recognized the utility of one of these barriers in the prevention of perineural adherence in mouse models [8]. However, the main drawback concerning these devices are their high cost.…”

Polymer Based Tissue Engineering Strategies for Neural Regeneration

“…[26][27][28][29][30] Further insight into its chemistry can be explored to modify these gels to provide sites for cell adhesion or extracellular matrix (ECM) molecules to allow cells to infiltrate into these scaffolds and thereby enhancing its potential applications. 26,[31][32][33][34][35] Poly (ethylene-co-vinylacetate): Poly (ethylene-covinyl acetate) (EVA) is a non-degradable, biocompatible and commercially available polymer. It has been extensively used for drug delivery applications, [36][37][38][39] while more recently it has been investigated for neural tissue engineering applications, such as repair of peripheral nerve injury.…”