A Simple Assay for Quantifying the Inducible Adherence of Neutrophils

Oez, Sadik; Welte, Karl; Platzer, E.; Kalden, Joachim R.

doi:10.1016/s0171-2985(11)80294-9

Cited by 35 publications

(21 citation statements)

References 17 publications

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“…The adherent cells were then stained by 0.2% solution of CV in PBS for 10 min at room temperature and washed thoroughly with tap water. The stain was dissolved in 33% acetic acid and the absorbance of the colored solution was measured by a spectrophotometer at 540 nm [41]. Photographs of the plasma-treated cells remaining at the bottom of plate wells were also taken, from which areas of cell adhesion and detachment could be determined.…”

Section: Adhesion Assaymentioning

confidence: 99%

The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells

et al. 2010

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Section: Adhesion Assaymentioning

confidence: 99%

The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells

et al. 2010

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“…The adhesiveness of leukocytes to various matrices (natural or synthetic) has been used for in vitro correlation of leukocyte activation, and the adherence to polystyrene is a widely used assay since the plastic surface behaves physiologically with respect to leukocyte adhesion [29]. Tissue infi ltration by blood derived macrophages implies adhesion of circulating monocytes to activated endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…The cell pellet was diluted in 2 mL PBS. Cell concentration was determined using manual hemocytometer and then diluted with PBS at a concentration of 2.5×10 Adhesion of peritoneal macrophages to plastic (polystyrene) was assessed with an in vitro spectrophotometric assay performed in 96 fl at bottom microtiter plates [29]. The assay was carried out in such a way that 50 μL of buffer was added per well, and then 50 μL of the macrophages suspension was placed in each well.…”

Section: Assays For Peritoneal Macrophages Adherence and Peroxide Promentioning

confidence: 99%

Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats

et al. 2017

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Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats.The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T 4 ), triiodothyronine (T 3 ), and thyroid stimulating hormone (TSH) were determined. The animals having "intermediate" concentrations of T 3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T 3 ≤1.55 nmol/L; T 4 ≤40 nmol/L), while PTU-groups included euthyroid rats (T 3 ≥1.70 nmol/L; T 4 ≥50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.

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“…Macrophage adherence was performed according to a previously published method [42] . Briefly, 96-well, flat-bottom tissue culture plates (Linbro, ICN Biomedicals, Aurora, Ohio, USA) were filled with 50 l of MEM, corticosterone (2 !…”

Section: Macrophage Adherencementioning

confidence: 99%

The Effects of Corticosterone and Beta-Endorphin on Adherence, Phagocytosis and Hydrogen Peroxide Production of Macrophages Isolated from Dark Agouti Rats Exposed to Acute Stress

Stanojević

Kuštrimović²,

Mitić³

et al. 2008

Neuroimmunomodulation

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Background: Given that stressful experiences can change the reaction to a subsequent exposure to stress, we tested the in vitro effects of the stress mediator corticosterone and the opioid peptide β-endorphin on the function of macrophages isolated from control rats and from rats exposed to electric tail shock stress (ES) or a stress-witnessing procedure (SW) 24 h earlier. Methods: Peritoneal macrophages isolated from control and stressed rats of the Dark Agouti (DA) strain were treated in vitro with corticosterone or β-endorphin and tested for adherence, phagocytosis and hydrogen peroxide release. Results: ES diminished adherence and SW decreased phagocytosis. The suppressive effect of corticosterone on phagocytosis was absent in rats exposed to ES and SW, while the suppressive effect of β-endorphin on adherence was not observed in rats exposed to SW. ES and SW did not affect H₂O₂ release, neither directly nor indirectly by changing macrophage response to corticosterone and β-endorphin in this test. Conclusions: In DA rats early macrophage activation steps, i.e. adherence and phagocytosis, were more sensitive to stress than their effector function, corresponding to H₂O₂ production. We suggest that neuroendocrine mediators of stress that converge on macrophages might have changed specific macrophage receptors or postreceptor events and alter their response to artificial stressors, represented by corticosterone and β-endorphin in vitro.

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A Simple Assay for Quantifying the Inducible Adherence of Neutrophils

Cited by 35 publications

References 17 publications

The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells

The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells

Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats

The Effects of Corticosterone and Beta-Endorphin on Adherence, Phagocytosis and Hydrogen Peroxide Production of Macrophages Isolated from Dark Agouti Rats Exposed to Acute Stress

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