A simple detection system for adenovirus receptor expression using a telomerase-specific replication-competent adenovirus

Sasaki, Tsuyoshi; Tazawa, Hiroshi; Hasei, Joe; Osaki, Shuhei; Kunisada, Toshiyuki; Yoshida, Aki; Hashimoto, Yuichi; Yano, Shuya; Yoshida, Ryuichi; Kagawa, Shunsuke; Uno, Futoshi; Urata, Yasuo; Ozaki, Toshifumi; Fujiwara, Toshiya

doi:10.1038/gt.2011.213

Cited by 9 publications

(14 citation statements)

References 43 publications

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“…We applied telomerase-specific OBP-401 to selectively label human neoplastic cells with GFP signals and confirmed its broad infectivity independent of EpCAM expression, which was consistent with observations from our previous reports that OBP-401 induced GFP expression in epithelial and mesenchymal types of tumour cells 24 25. Recent studies demonstrated that highly metastatic tumour cells are involved in EpCAM-positive and EpCAM-negative subpopulations of CTCs in the blood of patients with breast cancer 18 19.…”

Section: Discussionsupporting

confidence: 87%

“…It is also worth noting that the OBP-401-based biological CTC capture system is applicable to the genetic analysis of CTCs with mesenchymal characteristics, including GISTs and osteosarcomas,25 although the CellSearch system is also useful for detection of epithelial CTCs. Approximately 80% of GIST cells harbour a mutation in the KIT gene,30 which is significantly associated with disease recurrence and poor outcomes 47.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the small-molecule tyrosine-kinase inhibitor imatinib has been shown to be effective against KIT-mutated GIST that is refractory to conventional chemotherapy 48. In contrast, bone and soft tissue sarcoma cells, which make up one of the most notorious types of malignant mesenchymal tumours, are also detectable as GFP-positive cells by OBP-401 infection 25. Frequent lung metastasis has been shown to be a poor prognostic factor in patients with osteosarcoma, but the potential of CTC enumeration in patients with osteosarcoma remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…We previously developed a green fluorescent protein (GFP)-expressing telomerase-specific replication-competent adenovirus (OBP-401, TelomeScan) that drives the adenoviral E1A and E1B genes under the hTERT gene promoter for telomerase-dependent virus replication. OBP-401 enables the visualisation of viable epithelial and mesenchymal types of human tumour cells with telomerase activity as GFP-positive cells 24 25. OBP-401-mediated GFP labelling is a useful method to detect viable CTCs in patients with gastrointestinal cancers26 27 and ovarian cancers 28.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fluorescence virus-guided capturing system of human colorectal circulating tumour cells for non-invasive companion diagnostics

Shigeyasu

Tazawa

Hashimoto

et al. 2014

Gut

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fluorescence virus-guided capturing system of human colorectal circulating tumour cells for non-invasive companion diagnostics

Shigeyasu

Tazawa

Hashimoto

et al. 2014

Gut

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“…19 Therefore the expression of CAR is important for efficient gene transfer. However, because of the absence or low expression of CAR on several types of cancer cells always happens, 20 the improvement of adenoviral vector in tropism is dramatically needed.…”

Section: Discussionmentioning

confidence: 99%

Selective effects of a fiber chimeric conditionally replicative adenovirus armed with hep27 gene on renal cancer cell

Lin

Qian

Liu

et al. 2016

Cancer Biology & Therapy

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ASBTARCTAdenoviruses mediated cancer gene therapies are widely investigated and show a promising effect on cancer treatment. However, efficient gene transfer varies among different cancer cell lines based on the expression of coxsakie adenovirus receptor (CAR). Hep27, a member of dehydrogenase/reductase (SDR) family, can bind to Mdm2, resulting in the attenuation of Mdm2-mediated p53 degradation. Here we constructed a fiber chimeric adenovirus carrying hep27 gene (F5/35-ZD55-Hep27), in which the fiber protein of 5-serotype adenovirus (Ad5) was substituted by that of 35-serotype adenovirus (Ad35), aiming to facilitate the infection for renal cancer cells and develop the role of hep27 in cancer therapy. We evaluated the CAR and CD46 (a membrane cofactor protein for Ad35) expression in four kinds of renal cancer cells and assessed the relationship between receptors and infection efficiency. 5/ 35 fiber-modified adenovirus had a much promising infectivity compared with Ad5-based vector in renal cancer cells. F5/35-ZD55-Hep27 had enhanced antitumor activity against human renal cancer cells compared to the other groups. Further, hep27 mediated p53 and cleaved-PARP upregulation and mdm2 downregulation was involved and caused increased apoptosis. Moreover, F5/35-ZD55-Hep27 significantly suppressed tumor growth in subcutaneous renal cancer cell xenograft models. Our data demonstrated that 5/35 fiber-modified adenovirus F5/35-ZD55-Hep27 transferred into renal cancers efficiently and increased p53 to induce cancer cell apoptosis. Thus 5/35 fiber-modified adenoviral vector F5/35-ZD55-Hep27 might a promising vector and antitumor reagent for renal cancer gene therapy.

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Human Metaplastic Breast Carcinoma and Decorin

Boström

Sainio

Eigėlienė

et al. 2017

Cancer Microenvironment

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Metaplastic breast carcinoma (MBC) is a rare subtype of invasive breast cancer and has poor prognosis. In general, cancers are heterogeneous cellular masses comprised of different cell types and their extracellular matrix (ECM). However, little is known about the composition of the ECM and its constituents in MBC. Decorin is a ubiquitous ECM macromolecule known of its oncosuppressive activity. As such, it provides an intriguing molecule in the development of novel therapeutics for different malignancies such as MBC. In this study, decorin immunoreactivity and the effect of adenoviral decorin cDNA (Ad-DCN) transduction were examined in MBC. Multiple immunohistochemical stainings were used to characterize a massive breast tumour derived from an old woman. Furthermore, three-dimensional (3D) explant cultures derived from the tumour were transduced with Ad-DCN to study the effect of the transduction on the explants. The MBC tumour was shown to be completely negative for decorin immunoreactivity demonstrating that the malignant cells were not able to synthesize decorin. Ad-DCN transduction resulted in a markedly altered cytological phenotype of MBC explants by decreasing the amount of atypical cells and by inhibiting cell proliferation. The results of this study support approaches to develop new, decorin-based adjuvant therapies for MBC.Electronic supplementary materialThe online version of this article (doi:10.1007/s12307-017-0195-8) contains supplementary material, which is available to authorized users.

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A simple detection system for adenovirus receptor expression using a telomerase-specific replication-competent adenovirus

Cited by 9 publications

References 43 publications

Fluorescence virus-guided capturing system of human colorectal circulating tumour cells for non-invasive companion diagnostics

Fluorescence virus-guided capturing system of human colorectal circulating tumour cells for non-invasive companion diagnostics

Selective effects of a fiber chimeric conditionally replicative adenovirus armed with hep27 gene on renal cancer cell

Human Metaplastic Breast Carcinoma and Decorin

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