A Simple, Fast, Low Cost, HPLC/UV Validated Method for Determination of Flutamide: Application to Protein Binding Studies

Determination of flutamide and two major metabolites using HPLC–DAD and HPTLC methods

“…In the same way, the developed HPLC method was compared with all the published HPLC methods [ 3 , 7 , 16 – 20 ] regarding the used chromatographic conditions and the resulted retention time. Comparison items and results are given in Table 8 .…”

“… Detection wavelength: 240 nm Retention time: 4 min Linearity range : 0.2–25 µg/mL HPLC determination of flutamide [ 18 ] Stationary phase: nucleosil C18 column Mobile phase: acetonitrile: water (60:40, v/v). Flow rate: 1 mL/min Retention time: 6.35 min Linearity range: 0.0125–0.625 µg/mL Determination of flutamide in pharmaceutical formulation [ 19 ] Stationary phase: Spheri-5 RP-18 column Mobile phase: acetonitrile: water (70: 30, v/v) Flow rate: 1 mL/min Detection wavelength: λ ex = 255 nm, λ em = 375 nm Retention time: 3.9 min Linearity range: 0.1–0.6 µg/mL HPLC determination of flutamide [ 20 ] Stationary phase: C8 column Mobile phase: methanol: acetonitrile: KH2PO4 (50 mM, pH = 3.2) (29:38:33, by volume) Flow rate: 1 mL/min Retention time: 2.9 min Linearity range: 0.0625–1.6 µg/mL …”

“…Other methods were published for determination of Flutamide including electrochemical [ 2 , 9 , 10 ], different spectrophotometric [ 2 , 8 , 11 – 14 ], spectrofluorimetric [ 15 ], and different chromatographic methods [ 2 , 3 , 16 – 20 ].…”

Characterization of the Effect of Drug-Drug Interaction on Protein Binding in Concurrent Administration of Sulfamethoxazol and Diclofenac Sodium Using Bovine Serum Albumin

“…Some recent high sensitive technologies like HPLC/UV analysis method can be adopted for quantitation and determination of the binding pattern of drugs to HSA. 28 A more detailed study including in vivo experiments is warranted in this respect. However, the results of the present study in combination with the current advances in the binding of sulfamethoxazole and diclofenac sodium, might be helpful in realizing the overall binding behavior of these two drugs with HSA.…”

Application of 19F NMR Spectroscopy for Content Determination of Fluorinated Pharmaceuticals

“…Chromatographic techniques such as thin layer chromatography [15, 16], HPLC [17–20], gas chromatography or capillary electrophoresis [21], and spectrophotometric methods such as UV spectroscopy [17, 22] and electroanalytical techniques [23] have been utilized for purity determination of fluorinated compounds. These methods generally require the use of reference substances, expensive columns, and large volumes of organic solvents and in some cases laborious and time-consuming sample preparative steps.…”