Characterization of the Effect of Drug-Drug Interaction on Protein Binding in Concurrent Administration of Sulfamethoxazol and Diclofenac Sodium Using Bovine Serum Albumin

Hossain, Kamal; Khatun, Amina; Rahman, Mahmudur; Akter, Nahid; Chowdhury, Sadia Afreen; Alam, SM Mahbubul

doi:10.15171/apb.2016.073

Cited by 13 publications

(7 citation statements)

References 16 publications

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“…The lower amount of bound TRP in the absence of TL against other drugs is caused by a lower total HSA concentration (Figure 3C). According to information in the literature, DCF binds to Site I with high affinity and Site II with low affinity [40,41]. IBU binds mainly to Site II with high affinity, but some articles also describe its binding to a fatty acid binding site located at the interface between the IIA and IIB domains [42,43].…”

Section: Resultsmentioning

confidence: 99%

Applicability of capillary electrophoresis‐frontal analysis for displacement studies: Effect of several drugs on l‐tryptophan and lidocaine binding to human serum albumin

Nevídalová

Michalcová

Glatz

2020

J of Separation Science

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The effective concentration of a drug in the blood, i.e. the concentration of a free drug in the blood, is influenced by the strength of drug binding onto plasma proteins. Besides its efficacy, these interactions subsequently influence the liberation, absorption, distribution, metabolism, excretion, and toxicological properties of the drug. It is important to not only determine the binding strength and stoichiometry, but also the binding site of a drug on the plasma protein molecule, because the co-administration of drugs with the same binding site can affect the above-mentioned concentration and as a result the pharmacological behavior of the drugs and lead to side effects caused by the change in free drug concentration, its toxicity. In this study, the binding characteristics of six drugs with human serum albumin, the most abundant protein in human plasma, were determined by capillary electrophoresis-frontal analysis, and the obtained values of binding parameters were compared with the literature data. The effect of several drugs and site markers on the binding of l-tryptophan and lidocaine to human serum albumin was investigated in subsequent displacement studies which thus demonstrated the usability of capillary electrophoresis as an automated high-throughput screening method for drug-protein binding studies.

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Section: Resultsmentioning

confidence: 99%

Applicability of capillary electrophoresis‐frontal analysis for displacement studies: Effect of several drugs on l‐tryptophan and lidocaine binding to human serum albumin

Nevídalová

Michalcová

Glatz

2020

J of Separation Science

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“…DIC is the most common NSAID used to decrease inflammation and arthritis-associated pain (4,5). It is absorbed quickly and completely after consumption and attaches to albumin in plasma (6). Gastrointestinal side effects are the most common abnormal complications of DIC, although nephrotoxicity and liver toxicity are also related to DIC intoxication (7,8).…”

Section: Introductionmentioning

confidence: 99%

Nephroprotective effect of silymarin against diclofenac induced renal damage and oxidative stress in male rats

Nouri

Heidarian

2019

J Herbmed Pharmacol

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Introduction: Diclofenac (DIC), a phenylacetic acid compound which belongs to nonsteroidal anti-inflammatory drugs (NSAIDs), is generally used for the treatment of various diseases such as rheumatoid arthritis, ankylosing spondylitis, acute muscle pain conditions and osteoarthritis. Overdose of DIC can lead to renal injuries in both experimental animal and human. Our research was done to assess the protective role of silymarin on renal damage induced by DIC in rats. Methods: Thirty-two Wistar rats were assigned to four groups (n=8/group). Group 1 was control group; animals in group 2 were administrated DIC; Groups 3 and 4 administrated DIC plus silymarin with doses of 100 mg/kg and 200 mg/kg, orally (p.o), respectively. Various biochemical, molecular, and histological parameters were evaluated in serum and tissue homogenate. Results: In the second group, the levels of kidney catalase (CAT), vitamin C and superoxide dismutase (SOD) remarkably reduced (P < 0.05) relative to the control group. Also, urea, creatinine (Cr), malondialdehyde (MDA), serum tumor necrosis factor-α (TNF-α) and gene expression of TNF-α in this group were noticeably elevated (P < 0.05) relative to the control group. Treatment with silymarin caused a remarkable elevation (P < 0.05) in vitamin C, SOD, CAT and a remarkable reduction (P < 0.05) in the content of MDA, urea, Cr, TNF-α gene expression and serum TNF-α in comparison with second group. Histological injuries were also ameliorated by silymarin administration. Conclusion: The results confirm that silymarin has an ameliorative role against renal damage and oxidative stress induced by DIC in male rats.

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“…Diclofenac is one of the most widely NSAID used to decrease inflammation and arthritis-associated pain [7] [8]. It is rapidly and completely absorbed after consumption and attaches to albumin in plasma [9]. Its side effects include nephrotoxicity and liver toxicity Normally, combinations of biochemical and physiological phenomena play a key role in kidney vulnerability and renal toxicity [12].…”

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confidence: 99%

Diclofenac-Induced Kidney Damage in Wistar Rats: Involvement of Antioxidant Mechanism

Abiola¹,

Adebayo²,

Babalola³

2019

JBM

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Kidney damage has been associated with administration diclofenac, a phenylacetic acid derivative belonging to the nonsteroidal anti-inflammatory drugs (NSAIDs), which is commonly used for the treatment of various diseases such as rheumatoid arthritis, ankylosing spondylitis, acute muscle pain conditions and osteoarthritis. This study investigated the exact mechanism of diclofenac in renal toxicity by determining the involvement of oxidative stress in rats. Adult male Wistar rats were divided into two groups of eight rats in each group and orogastrically treated for three days. Group 1 served as the normal control and received normal saline (0.9% w/v) and group 2 received 40 mg/kg body weight of diclofenac for three days. Administration of diclofenac caused degeneration of the kidney of rats as evidenced by significant elevation in the serum levels of creatinine, urea, albumin, uric acid, protein and electrolytes and the activities of renal-5'-nucleotidase and glucose-6-phosphate-dehydrogenase (G6PDH) compared with control. Furthermore, administration of diclofenac decreased the activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione-S-transferase (GST) and the level of glutathione with concomitant increase in hydrogen peroxide (H 2 O 2) and malondialdehyde (MDA) levels in the kidney of the diclofenac treated groups compared with control. These findings reveal that administration of diclofenac may impair kidney functions through induction of oxidative stress.

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Characterization of the Effect of Drug-Drug Interaction on Protein Binding in Concurrent Administration of Sulfamethoxazol and Diclofenac Sodium Using Bovine Serum Albumin

Cited by 13 publications

References 16 publications

Applicability of capillary electrophoresis‐frontal analysis for displacement studies: Effect of several drugs on l‐tryptophan and lidocaine binding to human serum albumin

Applicability of capillary electrophoresis‐frontal analysis for displacement studies: Effect of several drugs on l‐tryptophan and lidocaine binding to human serum albumin

Nephroprotective effect of silymarin against diclofenac induced renal damage and oxidative stress in male rats

Diclofenac-Induced Kidney Damage in Wistar Rats: Involvement of Antioxidant Mechanism

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