A simple high performance liquid chromatography (HPLC) assay for aciclovir and ganciclovir in serum

Determination of aciclovir and ganciclovir in human plasma by liquid chromatography–spectrofluorimetric detection and stability studies in blood samples

“…Nevertheless, a number of analytical methods have been proposed for the measurement of ganciclovir in biological fluids by high performance liquid chromatography (HPLC), but only a few enable the measurement of aciclovir and/or ganciclovir with the same assay, and none have considered the influence of the metabolite of aciclovir, 9-carboxymethoxymethylguanine (CMMG) [9][10][11] that can potentially interfere with ganciclovir. In fact, CMMG, which has been already analysed together with aciclovir by Svensson et al [12], is at risk of co-eluting with ganciclovir when the latter is used as internal standard, or when previously or subsequently used in the same patient.…”

Determination of acyclovir in human serum by high-performance liquid chromatography using liquid–liquid extraction and its application in pharmacokinetic studies

“…Several HPLC methods [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] have been published for determination of acyclovir in human serum using UV or fluorescence detection. Since acyclovir is a polar compound and soluble in aqueous medium and practically insoluble in most of organic solvents, protein precipitation with perchloric acid [6][7][8]12,16,17] or solid phase extraction [9][10][11]14,15,20] are applied for pre-treatment of the drug in serum samples. While the sensitivity of analysis is significantly reduced due to dilution of the samples after deproteinization, injection of the acid supernatant after precipitation of proteins by perchloric acid leads to numerous late-eluting peaks and significant reduction of the lifetime of analytical column.…”

Development, evaluation and application of an isocratic high-performance liquid chromatography (HPLC) assay for the simultaneous determination of aciclovir and its metabolite 9-carboxymethoxymethylguanine in human serum and cerebrospinal fluid

“…The HPLC method was based on that developed for ACV [7]. Chromatography was performed on a Techsphere 5 m C-18 100 mm × 4.6 mm HPLC column (Chromacol Ltd., Welwyn Garden City, UK).…”