A simple isocratic HPLC method for the simultaneous determination of curcuminoids in commercial turmeric extracts

Wichitnithad, Wisut; Jongaroonngamsang, Nutthapon; Pummangura, Sunibhond; Rojsitthisak, Pornchai

doi:10.1002/pca.1129

Cited by 170 publications

(100 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The percentage curcumin estimated by the proposed method was in good agreement with natural composition ratio of curcumin found in turmeric extracts 25 (curcumin 76%, demethoxycurcumin 19% and bisdemethoxycurcumin 5%) used in clinical trials. [26][27][28] …”

Section: Methods Validationmentioning

confidence: 99%

Development and Validation of UV-Spectrophotometric method for the Estimation of Curcumin in Standardised Polyherbal Formulations

Singh¹,

Avupati²

2017

JYP

View full text Add to dashboard Cite

Objective: We aimed to develop and validate a simple UV-spectrophotometric method relates to the estimation of curcumin in standardised polyherbal formulations. Methods: Pure curcumin was received as generous gift sample from Andhra University, India, all solvents and reagents of analytical reagent (AR) grade were procured from local suppliers and readily used for the method development. The method validation parameters were evaluated as per International Conference on Harmonization (ICH) guidelines. Further, this method was applied for the assay of curcumin in marketed product Live-well™, CUMIN (standardised turmeric extract (STE) fortified with standardised black pepper (SBE) extract) capsules using UV-spectrophotometric method. Results and Discussion: It was confirmed from the results of method validation characteristics such as specificity, linearity, range, precision, accuracy, and robustness were keeping within the limits of acceptance criteria defined under ICH guidelines, 76.0560% of curcumin was estimated to be present in each Live-well™, CUMIN capsule containing a minimum 95% of the total curcuminoids (label claimed). Conclusion:In summary, our method was found to be reliable in detecting the unknown percentage of curcumin in Live-well™, CUMIN capsule formulation. Also, the method was proved to be specific, linear, precise, accurate and robust to use for routine analysis of polyherbal formulations containing curcumin as their principal component in the extracts.Key words: UV-spectrophotometric method, Curcumin, ICH Guidelines, Live-well™, CUMIN. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

show abstract

Section: Methods Validationmentioning

confidence: 99%

Development and Validation of UV-Spectrophotometric method for the Estimation of Curcumin in Standardised Polyherbal Formulations

Singh¹,

Avupati²

2017

JYP

View full text Add to dashboard Cite

show abstract

“…While the yellow pigment of curcuminoids may suggest that simplified spectrophotometric measurements for total curcuminoids could be used, it is essential to ensure that the three curcuminoids are identified, confirmed, and quantified after solvent extraction to ensure sufficient quality control of products (Taylor and McDowell 1992). The most suitable method to quantitation of curcuminoids is with HPLC with UV-Vis and/or mass spectrometric detection (Jayaprakasha et al 2002;Jiang et al 2006;Wichitnithad et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Curcuminoids in Turmeric Roots and Supplements: Method Optimization and Validation

et al. 2015

View full text Add to dashboard Cite

Curcuma longa L. rhizomes are used extensively as a spice in food preparations and dietary supplements for their anti-inflammatory and antioxidant properties. An expert review panel (ERP) evaluated analytical methods for the quantitation of individual curcuminoids for the purpose of identifying a method for official method status. It was requested that several modifications be undertaken to improve method performance prior to subjecting the chosen method to a singlelaboratory validation. Two separate Plackett-Burman factorial studies were used to identify factors that contributed to the chromatographic separation and extraction of curcuminoids. Significant factors were further optimized to produce the improved HPLC method for curcuminoid separation. This method was then subjected to a single-laboratory validation according to the AOAC International guidelines for linearity, detection limits, precision, and accuracy. The two most significant factors impacting the quantitation of curcuminoids were column temperature and extraction solvent, which were optimized to 55°C and 100 % methanol, respectively. The validation was performed on 12 raw materials and finished products containing turmeric roots. The method precision was reported using HorRat values which were within recommended ranges of the AOAC guidelines. Overall accuracy of the method was accessed at three separate levels for each analyte and ranged from 99.3-100.9 %. The validated method is suitable for quantitation of individual curcuminoids in turmeric raw materials and finished products and is recommended for consideration as an official method by the AOAC International.

show abstract

“…These limitations compromise the accuracy of quantification of these methods, reducing their effectiveness during application. Wichitnithad et al [16] and Jadhav et al [17] have described the best separation of the three principle curcuminoids to date.…”

Section: Linearitymentioning

confidence: 99%

Purity Determination of Three Curcuminoids Found in Ten Commercially Available Turmeric Dietary Supplements Using a Reverse Phase HPLC Method

Shervington¹,

Ingham²,

Shervington³

2016

Nat Prod Chem Res

View full text Add to dashboard Cite

The main active component present in turmeric is curcumin and is subsequently responsible for some of its therapeutic effects. Turmeric, however, contains two other active molecules, namely demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC). Collectively, these three molecules are known as curcuminoids. Commercially available dietary supplements vary in these components. In this study the curcuminoids were analyzed using a reverse phase ion-pairing HPLC method. UV detection was used to detect the three curcuminoids at a single wavelength of 430 nm. The validation of the method was carried to ICH guidelines. Good repeatability of the method was achieved at concentrations of curcumin, DMC and BDMC equivalent to 136, 148 and 162 µM, respectively, with R.S.D. values of 2.25, 1.93 and 1.64%, respectively. Similarly, good reproducibility and linearity of the method was obtained for curcumin, DMC and BDMC. On completion of the validation, 10 commercially available turmeric dietary supplements were analyzed for their curcuminoid content. Results showed two of the turmeric dietary supplements to contain near pure curcumin. The other supplements tested contained significant amounts of DMC and BDMC. Similarly, there was a significant increase in curcuminoid content between supplements that contained turmeric extract and those that contained raw turmeric. Interestingly, supplements containing turmeric extract contained a significantly higher proportion of curcumin compared to DMC and BDMC, reflected in the ratio of curcumin: DMC: BDMC.

show abstract

A simple isocratic HPLC method for the simultaneous determination of curcuminoids in commercial turmeric extracts

Abstract: The method was found to be simple, accurate and precise and is recommended for routine quality control analysis of turmeric extracts containing the three curcuminoid compounds as the main principles in the herb.

Cited by 170 publications

References 21 publications

Development and Validation of UV-Spectrophotometric method for the Estimation of Curcumin in Standardised Polyherbal Formulations

Development and Validation of UV-Spectrophotometric method for the Estimation of Curcumin in Standardised Polyherbal Formulations

Curcuminoids in Turmeric Roots and Supplements: Method Optimization and Validation

Purity Determination of Three Curcuminoids Found in Ten Commercially Available Turmeric Dietary Supplements Using a Reverse Phase HPLC Method

Contact Info

Product

Resources

About