A simple kinetic model for myeloma cell culture with consideration of lysine limitation

Abstract

JournalBioprocess and biosystems engineering AbstractA simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.

Process model comparison and transferability across bioreactor scales and modes of operation for a mammalian cell bioprocess

“… The cell death rate, k d , was modeled using the kinetic expression in Eq. 8,11, 12 where the parameter k d,max is the maximum death rate, and k μ is the intrinsic death rate. The rate of consumption of glucose was represented by Eq.…”

“…It is an unstructured, nonsegregated, mechanistic model. There are a number of examples of this type of model presented in the literature 6–12. The specific growth and death rates are generally modeled using Monod‐type functions based on nutrient and metabolite concentrations.…”

Model-based estimation of optimal dissolved oxygen profile in Agrobacterium sp. fed-batch fermentation for improvement of curdlan production under nitrogen-limited condition

“…The MSE value can be calculated by the sum square errors divided by the length of the actual data period [27]. (22) where f i , y i, y , and n t are the model data, experimental data, mean experimental data and length of actual data period, respectively.…”

“…In contrast to xanthan and pullulan fermentations, curdlan fermentation is a typical two-stage process, and curdlan can only be produced under nitrogen-limited conditions. A nitrogen-controlled cell growth model has been constructed to describe CHO cell growth [22,23]. A modified Monod kinetic model that incorporates nitrogen-controlled growth with heterologous substrate was also constructed to describe cell growth and curdlan fermentation:…”

Model‐based optimization of the feeding policy of a fluidized bed bioreactor for mercury uptake by immobilized Pseudomonas putida cells

“…Pyun et al [43] ; Lee and Hong [44] Max (productivity = prod. mass/batch time) + max (yield) + min (inducer) Aminoacids (Lysine) Liu et al [45] ; Taras and Woinaroschy [46] ; Sarkar and Modak [47] ; Logist et al [48] Max (productivity) + min (purification costs) Fructose by glucose isomerization Han et al [49] Concomitant production of two enzymes: max (ENZ-1) + min(ENZ-2)…”