A simple method for determination of deoxynivalenol in cereals and flours

Kotal, F; Radová, Zuzana

doi:10.17221/3511-cjfs

Cited by 21 publications

(8 citation statements)

References 18 publications

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“…HPLC-UV is preferable instrument for quantification. Shepherd et al monitored DON standard solutions stability at 220 nm [35]; Visconti et al detected NIV, DON, fusarenone X (FusX) and 3-AcDON at 225 nm [36]; Kotal and Ok-at 218 nm [37,38]; Yang-at 224 nm [39]. Within the European Commission project aimed at producing and certifying calibrants for the determination of B-trichothecenes, Krska et al applied a wide spectrum of analytical techniques for the estimation of purity and contaminants levels in standard solutions of DON, 3-AcDON, 15-AcDON and NIV [40].…”

Section: Type B Trichothecenesmentioning

confidence: 99%

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Kiseleva¹,

Chalyy²,

Седова³

et al. 2020

Toxins

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Standard solutions of mycotoxins prepared in RP HPLC solvents from neat standards are usually used for analytical method development. Multi-mycotoxin HPLC-MS/MS methods necessitate stability estimation for the wide spectrum of fungal metabolites. The stability of individual diluted stock standard solutions of mycotoxins in RP-HPLC solvents and multi-analyte HPLC-MS/MS calibrants was evaluated under standard storage and analysis conditions. Individual stock standard solutions of aflatoxins, sterigmatocystin, A-and B-trichothecenes, zearalenone and its analogues, ochratoxin A, fumonisins, Alternaria toxins, enniatins and beauvericin, moniliformin, citrinin, mycophenolic, cyclopiazonic acids and citreoviridin were prepared in RP-HPLC solvents and stored at −18 • C for 14 months. UV-spectroscopy was utilized to monitor the stability of analytes, excluding fumonisins. The gradual degradation of α-, β-zearalenol and α-, β-zearalanol in acetonitrile was detected. Aflatoxins and sterigmatocystin, zearalenone, Alternaria toxins, enniatins and beauvericin, citrinin, mycophenolic, cyclopiazonic acids and citreoviridin can be referred to as stable. The concentration of the majority of trichothecenes should be monitored. Diluted multi-mycotoxin standard in water/methanol (50/50 v/v) solutions acidified with 0.1% formic acid proved to be stable in silanized glass at 23 • C exposed to light for at least 75 h (CV ≤ 10%). An unexpected manifestation of MS/MS signal suppression/enhancement was discovered in the course of multi-mycotoxin standard solution stability evaluation. Key Contribution:The stability of the wide spectrum of mycotoxins in individual standard solutions in RP-HPLC solvents and multi-analyte HPLC-MS/MS calibrants under standard storage and analysis conditions is discussed basing on experimental and literature data.

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Section: Type B Trichothecenesmentioning

confidence: 99%

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Kiseleva¹,

Chalyy²,

Седова³

et al. 2020

Toxins

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“…A previous study showed that for isolation and increases maximum absorbance in UV range DON was passes through immunoaffinity column,[13] whereas in the present study DON was separated using alumina-silica-activated charcoal (1:1:1), which is more economic and easier.…”

Section: Discussionmentioning

confidence: 85%

Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography

Gupta

Chattopadhyay

Chaurasia

et al. 2011

Pharmaceutical Methods

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Deoxynivalenol (DON) is a mycotoxin produced by food contamination. It is a pharmacologically active compound that acts on the serotonin receptor, leading to several neuroendocrine and hematological disorders. In this article we describe a simple, accurate, and sensitive method for the quantification of DON. DON was quantified using a Phenomenex® ODS analytical C18 column (150 mm × 46 mm, 5 μm) with a mobile phase composed of mixture of water-acetonitrile-methanol (5:4:1, v/v/v) at a flow rate of 1.5 ml/min and at 254 nm in an ultraviolet (UV) detector The method has been validated with isolated samples of DON and provides a tool for the control of substandard and counterfeit commercial food products.

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“…All grain samples were ground in an Ultra Centrifugal Mill ZM 200 (Retsch, Germany) with 0.8 mm sieve. The ground spring wheat samples were analysed according to the method previously validated for the determination of DON in cereals with some modifications (Kotal, Radova, 2002). For extraction, 10 g of ground sample was placed into 100 ml glass bottle and then 40 ml deionized water and 2 g PEG were added.…”

Section: Methodsmentioning

confidence: 99%

The fate of deoxynivalenol and its derivatives in spring wheat whole-grain flour during storage

Janavičienė

Mankevičienè

Kochiieru

2020

Zemdirbyste-Agriculture

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A study was conducted at Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry to explore the quantitative changes in type B trichothecenes: deoxynivalenol (DON), 3-acelyt-deoxynivalenl (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON), in spring wheat whole-grain flour as influenced by storage period and temperature. Samples with different concentrations of DON and its metabolites were selected and stored in two controlled climate chambers at different temperatures (18°C and 28°C) at the same ambient air humidity (~80%). The initial concentration ranged from 1246 to 4581 µg kg-1 for DON, from 440 to 820 µg kg-1 for 3-ADON and from 88 to 141 µg kg-1 for 15-ADON. The samples were analysed before the storage experiment and then after 4 and 8 weeks of storage. The findings of the DON retention showed no significant differences between the storage periods and temperatures; however, it was found that over time the concentrations of DON in the samples decreased. There were found significant differences (P < 0.05) in 3-ADON concentrations between storage periods and temperatures. Over time, 3-ADON concentrations decreased in the samples. Statistically significant increases in 15-ADON concentrations were identified over this period. Within 60 days of storage, the concentrations of DON decreased by 16% and 33%, 3-ADON-by 60% and 100%, and those of 15-ADON increased by 63% and 96% compared to the initial levels, depending on the combination of the experimental factors. The results of this study led to the conclusion that storage of spring wheat flour for several months at 80% relative humidity, at 18°C and 28°C temperatures did not have significant influence on the retention level of DON. However, the levels of DON derivative 3-ADON significantly decreased and the concentrations of 15-ADON significantly (almost twice) increased during flour storage and aging.

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A simple method for determination of deoxynivalenol in cereals and flours

Cited by 21 publications

References 18 publications

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography

The fate of deoxynivalenol and its derivatives in spring wheat whole-grain flour during storage

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