Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography

Gupta, Vikas; Chattopadhyay, Pronobesh; Chaurasia, Asshwani Kumar; Gogoi, Hemanta Kumar; Singh, Lokendra; Kalita, Mohan Ch.

doi:10.4103/2229-4708.81087

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…To determine the type of TrMTs produced by the studied strains, fungal cultures were grown on a MYRO liquid medium [ 51 ] at 25 °C and 220 rpm for 5 days. The ability of isolates to produce DON and its monoacetylated derivatives was determined by reverse phase high pressure liquid chromatography of the culture filtrate supernatant separated from the mycelium by centrifugation [ 52 , 53 ]. An 8 mL aliquot of the culture liquid supernatant was diluted with a acetonitrile : water (1 : 1) mixture to 10 mL and passed through a 0.22 μm Millipore membrane filter; 10 μL of the sample was introduced into the injector of a Waters 1525 Breeze HPLC system equipped with a Waters 2487 UV detector (Waters, USA).…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections

et al. 2018

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We performed a three-locus phylogenetic analysis of Fusarium strains presumably capable of trichothecene production, which were deposited in the Russian national collections. The intra- and interspecific polymorphism of partial sequences of the translation elongation factor 1 alpha (TEF1α) gene and two genes from the trichothecene cluster TRI5 and TRI14 was studied. A study of 60 strains of different origins using DNA markers confirmed, and in the case for several strains, clarified their taxonomic characteristics. As a result, a strain of F. commune (F-900) was identified in Russia for the first time. Furthermore, the strain F-846 proved to be phylogenetically distinct from any of the known Fusarium species. F. equiseti strains from Northwest Russia were found to belong to the North European group (I), whereas a strain from the North Caucasus – to the South European one (II). Partial TRI14 sequences from 9 out of 12 species were determined for the first time. Their comparative analysis demonstrated a relatively high level of intraspecific variability in F. graminearum and F. sporotrichioides, but no correlation between the sequence polymorphism and the geographic origin of the strains or their chemotype was found. Specific chemotypes of trichothecene B producers were characterized using two primer sets. The chemotyping results were verified by HPLC.

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Section: Methodsmentioning

confidence: 99%

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections

et al. 2018

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“…The corn was then incubated for seven days at room temperature. After seven days, the fungal growth was stopped by dry sterilization at a temperature of 170 °C for 2 h (Gupta et al, 2011).…”

Section: Production Of Don In Corn Grainsmentioning

confidence: 99%

Local strains Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1 as a reducing and detoxifying agents for deoxynivalenol

Arifin¹,

Setyabudi²,

Sardjono³

2019

MJM

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Aims: Deoxynivalenol is a type B trichothecene produced by Fusarium graminearum that can cause serious health problems in human and livestock. The present study aimed to reduce and detoxify deoxynivalenol using a local strain Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1. Methodology and results: Corn as solid substrate artificially inoculated with F. graminearum bio 163252 to produce deoxynivalenol. Deoxynivalenol contaminated corn then inoculated with A. oryzae KKB4 and R. oryzae KP1R1. During fermentation, a decrease in deoxynivalenol levels is analyzed including loss of dry matter and glucosamine content. Deoxynivalenol was extracted from the substrate by solid phase extraction and quantified using high-performance liquid chromatography. The reduction of deoxynivalenol by A. oryzae KKB4 and R. oryzae KP1R1 were 65.91% and 56.82%, respectively after ten days of fermentation. Toxicity analysis revealed that residues of deoxynivalenol were not toxic to growth of Saccharomyces cerevisiae cells. Conclusion, significance and impact of study: Local strains A. oryzae KKB4 and R. oryzae KP1R1 were able to reduce and detoxify deoxynivalenol in solid substrates. This study provides supporting data to control mycotoxin that is critical for food and feed safety.

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“…Re-dissolving of the obtained residue was finalized in 1ml of water: acetonitrile: methanol (5:4:1). For purification, every single extract of the 17 was filtered through a separate Whatman Tm filter and the total amount of solution yielded was used for the HPLC analysis (17).…”

Section: Introductionmentioning

confidence: 99%

First Report of Three kinds of Mycotoxins Dioxynivalenol, Nivalenol and Fumonisin B2 in Seeds of Seven Wheat Cultivars in Iraq.

Minati

2019

Iraqi J. Vet. Med.

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This study was conducted to detect and quantify three mycotoxins Dioxynivalenol, Nivalenol and Fumonisin B2 in seeds of seven wheat cultivars planted in 17 wheat fields in Basra province, Iraq. This was done by using High Performance Liquid chromatographs analysis. The results revealed that Fumonisin B2 was the predominant mycotoxin, which present in 10 fields. The lowest concentration rate of this mycotoxin was 110 µg/Kg and the maximum was 11,228 µg/Kg. Dioxynivalenol as a trichothecene was in the second level detected in 6 fields with a minimum concentration of 8 µg/Kg and a maximum of 1,060 µg/Kg. Nivalenol was found only in 4 fields ranging from 272-1900 µg/Kg. Fumonisin B2 Only three fields showed co-occurrence of two mycotoxins (Fumonisin B2 and Nivalenol) in each, but with various concentration rates. The seven cultivars tested in this study were varied in their reactions to subjected mycotoxins. Adana 99 (A. 99) cultivar showed the highest concentration rate of both Fumonisin B2 and Nivalenol, which present with average percentage of 64% and 58% respectively. While, for Dioxynivalenol, Ebaa 99 (E.99) was on the top, it occurred in 6 fields ranging from 8-1060 µg/Kg with an average percentage of 43%.

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Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography

Cited by 3 publications

References 13 publications

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections

Local strains Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1 as a reducing and detoxifying agents for deoxynivalenol

First Report of Three kinds of Mycotoxins Dioxynivalenol, Nivalenol and Fumonisin B2 in Seeds of Seven Wheat Cultivars in Iraq.

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