A simple method for local delivery of various substances to the rat neuromuscular system

Connold, A.L.; Greensmith, Linda; Tyč, F.; Vrbová, Gerta

doi:10.1016/s1385-299x(96)00010-4

Cited by 13 publications

(11 citation statements)

References 11 publications

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“…The preparation of implants was performed by modification of the method of Connold et al (1997). Preparation included incorporation of the test compound into a silicon rubber solution (Dow Corning, 3140 RTV), which solidifies on contact with air.…”

Section: Methodsmentioning

confidence: 99%

“…To explore the role of E‐24.11 in the PNS and investigate whether E‐24.11 may be associated with peripheral nerve development and myelination, we have developed an in vivo method by which to probe the effects of possible inhibitors or enhancers on the process of myelination. The method we devised is based on a modification of a technique that has been widely used in studies of the developing neuromuscular system (Connold et al, 1997). Using this system, we examined the effect of two test compounds, namely phosphoramidon (PR), an inhibitor of E‐24.11 and EDTA, which is a more general inhibitor of Zn 2+ ‐metalloproteases.…”

Section: Introductionmentioning

confidence: 99%

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Cervical adenocarcinoma and squamous cell carcinoma incidence trends among white women and black women in the United States for 1976–2000

Wang

Sherman

Hildesheim

et al. 2004

Cancer

394

260

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BACKGROUNDAlthough cervical carcinoma incidence and mortality rates have declined in the U.S. greatly since the introduction of the Papanicolaou smear, this decline has not been uniform for all histologic subtypes. Therefore, the authors assessed the differential incidence rates of squamous cell carcinoma (SCC) and adenocarcinoma (AC) of the cervix by race and disease stage for the past 25 years.METHODSData from nine population‐based cancer registries participating in the U.S. Surveillance, Epidemiology, and End Results (SEER) Program were used to compute incidence rates for cervical carcinoma diagnosed during 1976–2000 by histologic subtype (SCC and AC), race (black and white), age, and disease stage (in situ, localized, regional, or distant).RESULTSIn black women and white women, the overall incidence of invasive SCC declined over time, and the majority of tumors that are detected currently are in situ and localized carcinomas in young women. The incidence of in situ SCC increased sharply in the early 1990s. AC in situ (AIS) incidence rates increased, especially among young women. In black women, invasive AC incidence rose linearly with age.CONCLUSIONSChanges in screening, endocervical sampling, nomenclature, and improvements in treatment likely explain the increased in situ cervical SCC incidence in white women and black women. Increasing AIS incidence over the past 20 years in white women has not yet translated into a decrease in invasive AC incidence. Etiologic factors may explain the rising invasive cervical AC incidence in young white women; rising cervical AC incidence with age in black women may reflect either lack of effective screening or a differential disease etiology. Cancer 2004;100:1035–44. © 2004 American Cancer Society.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cervical adenocarcinoma and squamous cell carcinoma incidence trends among white women and black women in the United States for 1976–2000

Wang

Sherman

Hildesheim

et al. 2004

Cancer

394

260

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Section: Materials and Methods Preparation Of Implants And Surgical Imentioning

confidence: 99%

“…To probe the effects of possible inhibitors or enhancers on the process of myelination in vivo, we developed a method based on a modification of a technique that has been widely used in studies of the developing neuromuscular system (Connold et al, 1997). The technique involves preparation of silicon implants containing the test compound as described in Materials and Methods and insertion of the implants around the sciatic nerve (Fig.…”

Section: Determination Of the Earliest Possible Implantation Timementioning

confidence: 99%

“…To explore the role of E-24.11 in the PNS and investigate whether E-24.11 may be associated with peripheral nerve development and myelination, we have developed an in vivo method by which to probe the effects of possible inhibitors or enhancers on the process of myelination. The method we devised is based on a modification of a technique that has been widely used in studies of the developing neuromuscular system (Connold et al, 1997). Using this system, we examined the effect of two test compounds, namely phosphoramidon (PR), an inhibitor of E-24.11 and EDTA, which is a more general inhibitor of Zn 2ϩ -metalloproteases.…”

Section: Introductionmentioning

confidence: 99%

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Novel method for studying myelination in vivo reveals that EDTA is a potent inhibitor of myelin protein and mRNA expression during development of the rat sciatic nerve

Meintanis

Thomaidou

Jessen

et al. 2004

Glia

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To probe the effects of possible inhibitors or enhancers of in vivo myelination, we have modified a technique widely used in studies of the developing neuromuscular system that involves incorporation of test compounds into a silicon rubber solution, which solidifies on contact with air. U-shaped rubber implants are inserted around the sciatic nerve of 1-day-old rats and left in place for 24-48 h. Sections from the region of the nerve lying within the implant, with or without the test compound, are then immunolabeled, examined with in situ hybridization or electron microscopy. Application of EDTA (440 microg/implant) in this way strongly suppressed the levels of the myelin-associated molecules protein P0, myelin basic protein (MBP), and galactocerebroside (Galc). mRNA levels for P0 and the myelin-related transcription factor Krox-20 were also reduced, further supporting association of the EDTA-induced effect with the myelinating Schwann cells. In contrast, no obvious differences were observed in either neurofilament (NF) protein or glial fibrillary acidic protein (GFAP) expression, suggesting absence of influence on axons or nonmyelinating Schwann cells. Despite the severely altered molecular composition of myelin in the presence of EDTA, examination in the electron microscope did not reveal any apparent ultrastructural changes in the myelin sheaths or nerve development. This work introduces a novel method for studying nerve development and shows that EDTA, which chelates divalent cations such as Ca(2+) and Mg(2+), strongly and selectively reduces levels of molecules, which, on postnatal days 1-4, are expressed in myelinating cells at much higher levels than in cells not engaged in myelination.

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Manipulating transmitter release at the neuromuscular junction of neonatal rats alters the expression of ChAT and GAP-43 in motoneurons

Sharp

Dekkers

Dick

et al. 2003

Developmental Brain Research

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A simple method for local delivery of various substances to the rat neuromuscular system

Cited by 13 publications

References 11 publications

Cervical adenocarcinoma and squamous cell carcinoma incidence trends among white women and black women in the United States for 1976–2000

Cervical adenocarcinoma and squamous cell carcinoma incidence trends among white women and black women in the United States for 1976–2000

Novel method for studying myelination in vivo reveals that EDTA is a potent inhibitor of myelin protein and mRNA expression during development of the rat sciatic nerve

Manipulating transmitter release at the neuromuscular junction of neonatal rats alters the expression of ChAT and GAP-43 in motoneurons

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