A simple method for the production of highly competent cells ofAgrobacterium for transformation via electroporation

McCormac, Alex C.; Elliott, M.C.; Chen, D F

doi:10.1007/bf02760816

Cited by 59 publications

(38 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anthocyanin pigmentation has been reported to be induced in immature embryos, shoot apical meristems, and aleurone layer of wheat, sorghum, barley and rice by the maize transcription factors of anthocyanin, C1/Lc or C1/Bperu (Bilang et al 1993, Casas et al 1993, McCormac et al 1998, Maekawa et al 2000. The present results also showed that C1/B-peru could activate the genes for anthocyanin synthesis in wheat coleoptiles.…”

Section: Discussionmentioning

confidence: 99%

“…However, GUS activity has been examined only by an in vitro assay procedure and detected histochemically as diffused blue spots. Recently, the modified gene of green fluorescent protein (GFP), expressed in plant cells and allowed non-lethal detection under a fluorescent microscope, has been used as a reporter (McCormac et al 1998).…”

Section: Introductionmentioning

confidence: 99%

“…(Chandler et al 1989, Ludwig andWessler 1990a). Induction of the anthocyanin pigment by these factors has been observed in heterologous plant tissues such as immature embryos of wheat and sorghum and rice aleurone layers (McCormac et al 1998, Casas et al 1993, Maekawa et al 2000. Anthocyanin pigmentation is autonomous and detected in the living tissue under a light dissection microscope.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transient Expression of Anthocyanin in Developing Wheat Coleoptile by Maize C1 and B-peru Regulatory Genes for Anthocyanin Synthesis.

et al. 2003

View full text Add to dashboard Cite

Maize transcription factors for the genes of anthocyanin synthesis pathway, C1/B-peru, were delivered into developing wheat coleoptiles by a particle bombardment method. Anthocyanin pigments were induced as discrete red spots and their number reached about 60 spots per coleoptile, compared to about 20 diffused blue GUS spots, which were induced by the GusA gene transferred concomitantly. Coleoptiles of seedlings collected 36 h after germination were most suitable tissue for the expression of delivered foreign genes. One to five µg of plasmid DNA for coating gold particles was sufficient for induction of anthocyanin and GUS, indicating that the transferred gene was expressed efficiently in coleoptile cells. Helium gas pressure (900, 1100, 1300 or 1500 pounds per square inch) and distances (10, 15, or 20 cm) between the stopping screen and coleoptile did not affect significantly the efficiency of gene transfer. Seedlings arranged in a circle with a 1-cm radius on a MS medium plate were targeted well by gold particles. The results showed that the wheat coleoptile was a good target tissue for transient assay of wheat genes and that C1/B-peru can be used as a reporter gene.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transient Expression of Anthocyanin in Developing Wheat Coleoptile by Maize C1 and B-peru Regulatory Genes for Anthocyanin Synthesis.

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Competent Agrobacterium tumefaciens (strain GV3101; pMP90) were prepared using the protocol of McCormac et al (1998). Arabidopsis (Col-0/vtc1) plants were transformed with Agrobacterium containing the NPR1-GFP construct (pBI 1.4t backbone; Kinkema et al, 2000) using a simple dip transformation technique (Clough and Bent, 1998).…”

Section: Plant Transformationmentioning

confidence: 99%

Ascorbic Acid Deficiency Activates Cell Death and Disease Resistance Responses in Arabidopsis

et al. 2005

View full text Add to dashboard Cite

Programmed cell death, developmental senescence, and responses to pathogens are linked through complex genetic controls that are influenced by redox regulation. Here we show that the Arabidopsis (Arabidopsis thaliana) low vitamin C mutants, vtc1 and vtc2, which have between 10% and 25% of wild-type ascorbic acid, exhibit microlesions, express pathogenesis-related (PR) proteins, and have enhanced basal resistance against infections caused by Pseudomonas syringae. The mutants have a delayed senescence phenotype with smaller leaf cells than the wild type at maturity. The vtc leaves have more glutathione than the wild type, with higher ratios of reduced glutathione to glutathione disulfide. Expression of green fluorescence protein (GFP) fused to the nonexpressor of PR protein 1 (GFP-NPR1) was used to detect the presence of NPR1 in the nuclei of transformed plants. Fluorescence was observed in the nuclei of 6-to 8-week-old GFP-NPR1 vtc1 plants, but not in the nuclei of transformed GFP-NPR1 wild-type plants at any developmental stage. The absence of senescence-associated gene 12 (SAG12) mRNA at the time when constitutive cell death and basal resistance were detected confirms that elaboration of innate immune responses in vtc plants does not result from activation of early senescence. Moreover, H 2 O 2 -sensitive genes are not induced at the time of systemic acquired resistance execution. These results demonstrate that ascorbic acid abundance modifies the threshold for activation of plant innate defense responses via redox mechanisms that are independent of the natural senescence program.The complex relationships between programmed cell death (PCD) and natural senescence observed during leaf development are far from understood. However, one clear distinction is that senescence in leaves is essentially reversible, but PCD is not (Thomas et al., 2003). The genetically programmed cell suicide events that comprise PCD are triggered by enhanced levels of reactive oxygen species (ROS; Chen and Dickman, 2004;Laloi et al., 2004;Wagner et al., 2004). However, senescence-enhanced genes are also expressed in response to ROS (Navabpour et al., 2003).While the chemical nature of ROS dictates that they are potentially harmful to cells, plants use ROS as second messengers in signal transduction cascades regulating diverse processes such as mitosis, tropisms, and cell death. It is now well accepted that ROS accumulation is crucial to plant development as well as defense (Foyer and Noctor, 2005a). ROS signal transduction will ensue only if ROS escape destruction by cellular antioxidants that determine the lifetime and specificity of the signal. Ascorbic acid (AA) and glutathione are the major redox buffers of the plant cells, and they themselves are also signal-transducing molecules that can either signal independently or further transmit ROS signals (Fig. 1). They are thus intrinsic to redox homeostasis and redox-signaling events (Foyer and Noctor, 2005b).ROS production is often genetically programmed, for example, during the hypersen...

show abstract

“…The NS3 gene was inserted into the multicloning site of SpeI/EcoRI-digested pGreen0229 to place it under the control of 35S promoter to give construct pGreen0229-35S:NS3 (35S-NS3). The 35S-NS3 was transferred into A. tumefaciens strain AGL1, with helper plasmid (pJIC) by electroporation (McCormac et al, 1998).…”

Section: Methodsmentioning

confidence: 99%

Effect of Rice stripe virus NS3 on Transient Gene Expression and Transgene Co-Silencing

Sohn¹,

Huh²,

Kim³

et al. 2011

The Plant Pathology Journal

View full text Add to dashboard Cite

Nonstructural protein 3 (NS3) encoded by RNA3 of Rice stripe virus (RSV), known to be a suppressor of gene silencing, was cloned and sequenced. The cloned NS3 gene is composed of 636 nucleotides encoding 211 deduced amino acids, and showed a high degree of similarity with the equivalent genes isolated from Korea, Japan and China. The NS3 gene promoted the enhancement of transient gene expression and suppressed transgene co-silencing. In the transient GFP expression via agroinfiltration, GFP expression was dramatically enhanced in terms of both protein yield and expression period in the presence of NS3. The highest accumulation of GFP protein reached to 6.8% of total soluble proteins, which corresponded to a two-fold increase compared to that obtained in the absence of NS3. In addition, NS3 significantly suppressed the initiation of GFP co-silencing induced by the additive GFP infiltration in GFP-transgenic Nicotiana benthamiana. The NS3 gene was also found to be a stronger suppressor than Cucumber mosaic virus 2b. These observations are believed to be derived from the strong suppressive effect of NS3 on gene silencing, and indicate that NS3 could be used as an effective enhancer for the rapid production of foreign proteins in plants.

show abstract

A simple method for the production of highly competent cells ofAgrobacterium for transformation via electroporation

Cited by 59 publications

References 5 publications

Transient Expression of Anthocyanin in Developing Wheat Coleoptile by Maize C1 and B-peru Regulatory Genes for Anthocyanin Synthesis.

Transient Expression of Anthocyanin in Developing Wheat Coleoptile by Maize C1 and B-peru Regulatory Genes for Anthocyanin Synthesis.

Ascorbic Acid Deficiency Activates Cell Death and Disease Resistance Responses in Arabidopsis

Effect of Rice stripe virus NS3 on Transient Gene Expression and Transgene Co-Silencing

Contact Info

Product

Resources

About