A simple technique for localizing consecutive fields for disector pairs in light microscopy: Application to neuron counting in rabbit spinal cord following spinal cord injury

Kaplan, Süleyman; Gökyar, Ahmet; Ünal, Bünyami; Tunç, Ayten Türkkanı; Bahadır, Ayşe; Aslan, Hüseyin

doi:10.1016/j.jneumeth.2005.02.012

Cited by 37 publications

(38 citation statements)

References 40 publications

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“…For stereological analysis (Gundersen and Jensen, 1987;Chagas et al, 2002, Kaplan et al, 2005 Gundersen and Jensen, (1987). The mean coefficient of variation for each group and the mean coefficient of error for stereological estimation of tissue volumes were observed in an acceptable level.…”

Section: Microscopy Imaging and Stereological Analysesmentioning

confidence: 82%

The stereological ratio of stromal/parenchymal components in postnatal prostate tissue of rats prenatally treated with diclofenac sodium

Kara

Ragbetli²,

Koyun³

et al. 2013

jecm

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Article HistorySince diclofenac sodium extends the pregnancy and affects the fetus, our study aimed to stereologically investigate the components of the rat prostate with prenatally treated with diclofenac sodium. The rats were left overnight to mate. Pregnant animals were kept in a room tempreture with normal light and dark cycles and fed with normal diet. After injections to sham and treated groups for 15 days, 18 male offsprings were housed in three groups (6 placebo; 6 control; 6 treated). The 20 weeks-old animals were perfused and the prostates removed and embedded in paraffin. Tissue sections at 5µm thickness were taken at regular intervals and stained with Hematoxylin-eosin and Masson trichrome. Cavalieri principle was used for volume fractions analysis. Stereological examination revealed 55% acinar epitelium and 45% connective tissue in both control and placebo groups, as well as 60% acinar epitelium and 40% connective tissue in the treated group. Prenatally applied diclofenac sodium did not cause a statistically significant change in the prostate stroma-parenchymal ratio in 20-week old rats compared to the control animals (p>0.05).

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Section: Microscopy Imaging and Stereological Analysesmentioning

confidence: 82%

The stereological ratio of stromal/parenchymal components in postnatal prostate tissue of rats prenatally treated with diclofenac sodium

Kara

Ragbetli²,

Koyun³

et al. 2013

jecm

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“…A test point grid was used to estimate area fraction of the tunica media (TM) including tunica intima, tunica adventitia (TA) and vessel cross sectional area (CSA) of vessel using a modified light microscope (Kaplan et al, 2001;Kaplan et al, 2005). A test point grid, printed on a transparent sheet ( Fig.…”

Section: Stereological Analysismentioning

confidence: 99%

“…A test point grid, printed on a transparent sheet ( Fig. 1), was inserted into the eyepiece and two dial indicators attached to the stage of microscope were used (Kaplan et al, 2001;Kaplan et al, 2005). Systematic random area sampling (200x200µm 2 ) on the section was done by means of dial indicators.…”

Section: Stereological Analysismentioning

confidence: 99%

Effects Of Pınealectomy On Morphologıcal Features Of Blood Vessel In Chıcken: An Electron Mıcroscopıc And Stereologıcal Study

TURGUT¹,

Kaplan²,

ÜNAL³

et al. 2009

jecm

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Although the effects of melatonin on vascular smooth muscle and vascular reactivity are described in last decade, the effects of melatonin on vascular tissues are still vague. The current study investigated the effects of pinealectomy on the blood vessel wall in the chicken. Fifteen chicks were divided into three groups, namely unoperated control, sham-operated and pinealectomized chicks. At the end of the experiment, a quantitative structural analysis of 15 sciatic artery samples from these groups was investigated. Effects of pinealectomy were quantified using volume fraction approach of stereological method. In this study, total cross-sectional area of the artery in pinealectomy group was significantly higher than those in unoperated control and sham-operated animals (p < 0.05). However, surgical pinealectomy procedure resulted in a significantly decreased thickness of the tunica adventitia of the vessel wall, in contrast to those of tunica media (p < 0.01). The present results indicate that pinealectomy had a vasodilator effect on the sciatic artery. In the light of current study, the structural changes in the vessel demonstrated by quantitative morphometric methods have been interpreted as a reflection of the role of melatonin on vessel wall and vascular reactivity, but this suggestion need to be validated in the human setting.

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“…This number is in an acceptable range for stereological analysis. [22,23] Two consecutive sections were mounted on each slide. Photographs of adjacent sections were taken with a digital camera at a magnification of 400×.…”

Section: Numerical Density and Total Number Of Glomerulimentioning

confidence: 99%

“…[23] The mean numerical density of glomeruli [Nv (glomeruli) ] per cm 3 was estimated using the following formula:…”

Section: Numerical Density and Total Number Of Glomerulimentioning

confidence: 99%

Does Haloperidol Have Side Effects on Histological and Stereological Structure of the Rat Kidneys?

et al. 2009

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Haloperidol, a typical antipsychotic, is the most commonly prescribed medication for the treatment of mental health problems such as agitation and psychosis. We attempted to determine the effects of haloperidol treatment on the kidneys of female rats. In addition, we aimed to estimate the numerical density, total number, and height of renal glomeruli and the volume and volumetric fractions of the cortex, medulla, and whole kidneys, and tried to determine whether there was a change in these stereological parameters depending on haloperidol treatment. Both the qualitative and quantitative histological features of the kidney samples were analyzed with conventional histopathological and modern stereological methods at the light microscopic level. The total number of glomeruli and numerical density of glomerulus in the haloperidoltreated groups was not changed by increasing the dose in comparison to the control group. The mean height of the glomerulus significantly increased, especially in low-dose groups. In the haloperidol-treated groups, the volumetric fractions of the cortex to the whole kidney of the rats were significantly decreased by increasing the dose. The volumetric fractions of the medulla to the whole kidney of the rats were increased significantly in parallel by the given dose. In addition, we present quantitative findings showing that haloperidol is associated with many alterations in rat kidneys. It was shown that haloperidol may lead to undesirable changes in the kidney after chronic treatment with especially high doses.

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A simple technique for localizing consecutive fields for disector pairs in light microscopy: Application to neuron counting in rabbit spinal cord following spinal cord injury

Cited by 37 publications

References 40 publications

The stereological ratio of stromal/parenchymal components in postnatal prostate tissue of rats prenatally treated with diclofenac sodium

The stereological ratio of stromal/parenchymal components in postnatal prostate tissue of rats prenatally treated with diclofenac sodium

Effects Of Pınealectomy On Morphologıcal Features Of Blood Vessel In Chıcken: An Electron Mıcroscopıc And Stereologıcal Study

Does Haloperidol Have Side Effects on Histological and Stereological Structure of the Rat Kidneys?

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