A high-voltage gain two-switch quasi-Z-source isolated DC/DC converter has been presented in this study. It consists of a quasi-Z-source network at its input, a push-pull square-wave inverter at its middle, and a voltage-doubler rectifier at its output. When coordinated appropriately, the new converter uses less switches, a smaller common duty cycle and less turns for the transformer when compared with existing topologies. Its size and weight are therefore smaller, whereas its efficiency is higher. It is therefore well-suited for applications, where a wide range of voltage gain is required like renewable energy systems, DC power supplies found in telecom, aerospace and electric vehicles. To demonstrate the performance of the proposed converter, a 400 V, 500 W prototype has been implemented in the laboratory. Efficiency of the prototype measured is found to vary from 89.0 to 97.4% when its input voltage changes from 44 to 82 V at full load.