A ‘smart’ aptamer-functionalized continuous label-free cell catch–transport–release system

Zhang, Bozhen; Wang, Canran; Du, Yingjie; Paxton, Rebecca Joanne; He, Ximin

doi:10.1039/d1tb00739d

Cited by 2 publications

(3 citation statements)

References 64 publications

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“…In addition, this transition behavior is reversible, meaning that the decrease in temperature below the TT will turn the thermoresponsive polymer to a more hydrophilic state again. 19 Based on this special capability, thermoresponsive polymers are well suited for use in temperature switches in the nanoscale, 20 controlled cell delivery, 21 smart textiles, 22 artificial muscles, 23 and catalyst carriers. 24 At present, the exploration of thermoresponsive polymer films is mainly based on acrylamide-based polymers, such as poly(N-isopropylacrylamide) (PNIPAM).…”

Section: ■ Introductionmentioning

confidence: 99%

Effect of Thermal Stimulus on Kinetic Rehydration of Thermoresponsive Poly(diethylene glycol monomethyl ether methacrylate)-block-poly(poly(ethylene glycol) methyl ether methacrylate) Thin Films Probed by In Situ Neutron Reflectivity

Metwalli

et al. 2022

Langmuir

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The kinetic rehydration of thin di-block copolymer poly(diethylene glycol monomethyl ether methacrylate)-block-poly(poly(ethylene glycol) methyl ether methacrylate) (PO 2 -b-PO 300 ) films containing two thermoresponsive components is probed by in situ neutron reflectivity (NR) with different thermal stimuli in the D 2 O vapor atmosphere. The transition temperatures (TTs) of PO 2 and PO 300 blocks are 25 and 60 °C, respectively. After the one-step stimulus (rapid decrease in temperature from 60 to 20 °C), the film directly switches from a collapsed to a fully swollen state. The rehydration process is divided into four steps: (a) D 2 O condensation, (b) D 2 O absorption, (c) D 2 O evaporation, and (d) film reswelling. However, the film presents a different rehydration behavior when the thermal stimulus is separated into two smaller steps (first decrease from 60 to 40 °C and then to 20 °C). The film first switches from a collapsed to a semiswollen state caused by the rehydrated PO 300 blocks after the first step of thermal stimulus (60 to 40 °C) and then to a swollen state induced by the rehydrated PO 2 blocks after the second step (40 to 20 °C). Thus, the kinetic responses are distinct from that after the one-step thermal stimulus. Both the time and extent of condensation as well as evaporation processes are significantly reduced in these two smaller steps. However, the final states of the rehydrated PO 2 -b-PO 300 films are basically identical irrespective of the applied thermal stimulus. Thus, the final state of thermoresponsive di-block copolymer films is not affected by the external thermal stimuli, which is beneficial for the design and preparation of sensors or switches based on thermoresponsive polymer films.

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Section: ■ Introductionmentioning

confidence: 99%

Effect of Thermal Stimulus on Kinetic Rehydration of Thermoresponsive Poly(diethylene glycol monomethyl ether methacrylate)-block-poly(poly(ethylene glycol) methyl ether methacrylate) Thin Films Probed by In Situ Neutron Reflectivity

Metwalli

et al. 2022

Langmuir

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“…For example, poly-N-isopropylacrylamide (PNIPAAm) provides a strategy for fabricating tunable temperature-active hydrogels. Zhang et al designed a DNA hydrogel incorporating PNIPAAm for the capture and release of immunoaffinity cells [ 63 ]. When the temperature rose to the common critical temperature of the thermosensitive material and the aptamer, the material contracted and the aptamer denatured to release the cells, enabling a temperature-controlled capture to release the cells ( Figure 3 C).…”

Section: Preparation and Classification Of Stimulus-responsive Dna Hy...mentioning

confidence: 99%

“…( C ) Schematic diagram of the reversible response of aptamer-functionalized hydrogels to temperature. Copyright (2021) RSC publications [ 63 ]. ( D ) Schematic diagram of a DNA hydrogel that responds to Ag + .…”

Section: Figurementioning

confidence: 99%

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Wang

Lai

et al. 2023

Biosensors

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Food safety has always been a major global challenge to human health and the effective detection of harmful substances in food can reduce the risk to human health. However, the food industry has been plagued by a lack of effective and sensitive safety monitoring methods due to the tension between the cost and effectiveness of monitoring. DNA-based hydrogels combine the advantages of biocompatibility, programmability, the molecular recognition of DNA molecules, and the hydrophilicity of hydrogels, making them a hotspot in the research field of new nanomaterials. The stimulus response property greatly broadens the function and application range of DNA hydrogel. In recent years, DNA hydrogels based on stimulus-responsive mechanisms have been widely applied in the field of biosensing for the detection of a variety of target substances, including various food contaminants. In this review, we describe the recent advances in the preparation of stimuli-responsive DNA hydrogels, highlighting the progress of its application in food safety detection. Finally, we also discuss the challenges and future application of stimulus-responsive DNA hydrogels.

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A ‘smart’ aptamer-functionalized continuous label-free cell catch–transport–release system

Abstract: Label-free cell sorting devices are of great significance for biomedical research and clinical therapeutics. However, current platforms for label-free cell sorting cannot achieve continuity and selectivity simultaneously, resulting in complex...

Cited by 2 publications

References 64 publications

Effect of Thermal Stimulus on Kinetic Rehydration of Thermoresponsive Poly(diethylene glycol monomethyl ether methacrylate)-block-poly(poly(ethylene glycol) methyl ether methacrylate) Thin Films Probed by In Situ Neutron Reflectivity

Effect of Thermal Stimulus on Kinetic Rehydration of Thermoresponsive Poly(diethylene glycol monomethyl ether methacrylate)-block-poly(poly(ethylene glycol) methyl ether methacrylate) Thin Films Probed by In Situ Neutron Reflectivity

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

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