A Solid-state Electrochemiluminescence Sensor for Detecting Glutathione with a Graphite-phase Carbon Nitride/Silica Modified Glassy Carbon Electrode

Abstract

A solid-state electrochemiluminescence (ECL) sensor for the detection of reduced glutathione (GSH) based on a g-C3N4/ SiO2 modified glass carbon electrode (GCE) has been developed in this research. The g-C3N4, which is employed as a luminophore, is simply prepared and exhibits an excellent ECL response. Mesoporous silica hollow spheres (SiO2) with a large specific surface area are introduced here to increase the loading amount of g-C3N4. Compared to a g-C3N4 modified GCE, the g-C3N4/SiO2 modified GCE displays a much higher ECL intensity. A high enhancement effect on the ECL intensity of g-C3N4/SiO2 modified GCE is obtained in the presence of GSH in the electrolyte. Moreover, the enhanced ECL intensity shows a good linear relationship to the GSH concentration in the range from 1.0 × 10 -7 to 5.0 × 10 -4 M, with a detection limit of 2.0 × 10 -8 M (6.1 ng/mL). Besides, the ECL sensor exhibits a good anti-interference ability and has been successfully applied in the detection of GSH in commercial samples. The proposed sensor provides a promising platform for life science.

Two near-infrared phosphorescent iridium(iii) complexes for the detection of GSH and photodynamic therapy

“…Conventional detection methods have been developed to detect the target, including HPLC, capillary electrophoresis and electrochemiluminescence analysis. [13][14][15] However, these methods suffer from complicated sample pre-treatment and cannot detect target analytes in living cells and tissues.…”

“…Conventional detection methods have been developed to detect the target, including HPLC, capillary electrophoresis and electrochemiluminescence analysis. 13–15 However, these methods suffer from complicated sample pre-treatment and cannot detect target analytes in living cells and tissues. Fluorescence detection has gradually become a powerful detection tool for various biologically active molecules because of its operational simplicity, high sensitivity, good selectivity and applicability in living cells.…”

Electrochemiluminescence Sensing

“…Shan et al provided graphite carbon nitride (g-C3N4)/mesoporous silica hollow spheres (SiO2) as a modified glassy carbon electrode for the detection of reduced glutathione (GSH). 10 Because mesoporous hollow spheres of SiO2 have a large specific surface area to immobilize a substantial amount of g-C3N4, the ECL intensity is significantly enhanced on the g-C3N4/SiO2 modified glassy carbon electrode. By utilizing the present ECL sensor, the detection limit of GSH was achieved at 20 nM.…”