A specific molecular beacon probe for the detection of human prostate cancer cells

Jiang, Yu; McGoldrick, Christopher A; Yin, Deling; Zhao, Jing; Patel, Vini; Brannon, Marianne; Lightner, Janet; Krishnan, Koyamangalath; Stone, William L.

doi:10.1016/j.bmcl.2012.04.055

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…The recovered solute was triturated three times with H 2 O and lyophilized to give a yellow solid (210 mg, 77% yield). Spectra were in accordance with that reported in literature …”

Section: Methodssupporting

confidence: 90%

Tunable, Functional Diblock Copolypeptide Hydrogels Based on Methionine Homologs

Negri

Gharakhanian

Deming

2019

Macromolecular Bioscience

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The preparation of new diblock copolypeptide hydrogels derived from homologs of l‐methionine, that is, l‐homomethionine and l‐6‐(methylthio)‐l‐norleucine is described. Compared to l‐methionine residues, use of l‐methionine homologs allow improved copolymerization with l‐leucine residues to give well‐defined block copolypeptides. These copolypeptides are subsequently modified using robust thioether alkylation reactions employing a variety of functional epoxides, which yield samples capable of forming transparent, self‐healing hydrogels in water. The facile variation of different functional epoxides for postpolymerization modification is found to allow predictable functionalization and tuning of hydrogel properties by the modification of simple precursors.

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“…The recovered solute was triturated three times with H 2 O and lyophilized to give a yellow solid (210 mg, 77% yield). Spectra were in accordance with that reported in literature …”

Section: Methodssupporting

confidence: 90%

Tunable, Functional Diblock Copolypeptide Hydrogels Based on Methionine Homologs

Negri

Gharakhanian

Deming

2019

Macromolecular Bioscience

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“…The carboxyl esterase is a biomarker of cancer38 and one of the major enzymes related to drug metabolism and pro-drug activation39. For example, human carboxyl esterase 2 is commonly expressed in tumour tissues and is correlated with the activation of anticancer drugs40.…”

Section: Resultsmentioning

confidence: 99%

A platform for designing hyperpolarized magnetic resonance chemical probes

et al. 2013

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Hyperpolarization is a highly promising technique for improving the sensitivity of magnetic resonance chemical probes. Here we report [15N, D9]trimethylphenylammonium as a platform for designing a variety of hyperpolarized magnetic resonance chemical probes. The platform structure shows a remarkably long 15N spin–lattice relaxation value (816 s, 14.1 T) for retaining its hyperpolarized spin state. The extended lifetime enables the detection of the hyperpolarized 15N signal of the platform for several tens of minutes and thus overcomes the intrinsic short analysis time of hyperpolarized probes. Versatility of the platform is demonstrated by applying it to three types of hyperpolarized chemical probes: one each for sensing calcium ions, reactive oxygen species (hydrogen peroxide) and enzyme activity (carboxyl esterase). All of the designed probes achieve high sensitivity with rapid reactions and chemical shift changes, which are sufficient to allow sensitive and real-time monitoring of target molecules by 15N magnetic resonance.

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“…Among them, biomolecular probes based on different fluorogenic substrates have received much attention due to the simplicity, speed, and sensitivity of their assays 10. Although these probes have been proven successful in carboxylesterase activity assays, the development of water‐soluble11 and synthetically readily accessible fluorogenic probes12 with improved assay sensitivity and selectivity is still desirable.…”

Section: Introductionmentioning

confidence: 99%

A Fluorogenic Probe with Aggregation‐Induced Emission Characteristics for Carboxylesterase Assay through Formation of Supramolecular Microfibers

Wang

Liu

et al. 2014

Chemistry An Asian Journal

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Herein, we report a novel fluorescent "light-up" probe useful for carboxylesterase assay that is based on a tetraphenylethylene derivative containing carboxylic ester groups. The specific cleavage of the carboxylic ester bonds by carboxylesterase results in the generation of a relatively hydrophobic moiety that self-assembles into supramolecular microfibers, thus giving rise to "turn-on" fluorescent signals. A high sensitivity towards carboxylesterase was achieved with a detection limit as low as 29 pM, which is much lower than the corresponding assays based on other fluorescent approaches.

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A specific molecular beacon probe for the detection of human prostate cancer cells

Cited by 8 publications

References 28 publications

Tunable, Functional Diblock Copolypeptide Hydrogels Based on Methionine Homologs

Tunable, Functional Diblock Copolypeptide Hydrogels Based on Methionine Homologs

A platform for designing hyperpolarized magnetic resonance chemical probes

A Fluorogenic Probe with Aggregation‐Induced Emission Characteristics for Carboxylesterase Assay through Formation of Supramolecular Microfibers

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