A stable L-form of the fish pathogen Aeromonas salmonicida

Abstract

A stable L‐form of Aeromonas salmonicida, which resulted from induction with benzylpenicillin, grew on brain heart infusion agar at 0–5°C. The L‐form was stored successfully for 10 months in phosphate‐buffered saline supplemented with 150 g l‐1 glycerol at ‐70°C. Reversion of the L‐form to parental‐type walled cells was achieved by transfer to brain heart infusion broth with incubation at 25°C.

Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post‐larval Penaeus vannamei (Bonne)

“…Western blotting was carried out according to the methods outlined by Gibb, McIntosh & Austin (1996). Primary antiserum (titre by whole cell agglutination = 1:2048) to formalin‐killed whole cells of V. harveyi E2 was produced in a New Zealand white female rabbit.…”