A Standardized Model of Partial Thickness Scald Burns in Mice

Cribbs, Randolph K.; Luquette, Mark; Besner, Gail E.

doi:10.1006/jsre.1998.5340

Cited by 42 publications

(29 citation statements)

References 21 publications

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“…The device does not cover the wound itself. Cribbs et al 4 use continuous sedation by adding benzodiazepines to the drinking water during the first days after the injury but do not mention the disadvantages of ongoing sedation. In vivo burn wound models in rats and other smaller rodents investigate the acute phase after the injury and have no dressings placed over the wound at all.…”

Section: Discussionmentioning

confidence: 99%

Innovative scald burn model and long-term dressing protector for studies in rats

Pfurtscheller

Petnehazy²,

Goessler³

et al. 2013

Journal of Trauma and Acute Care Surgery

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R ats are frequently used for experimental studies in burns.Scalding is the easiest mechanism for provoking a dermal burn; the ability to vary water temperature, time of exposure, and the burned area make this method effective for reproducing almost any kind of thermal wound. Last but not least, rats are readily available, inexpensive, and easy to manage.In general, a scald injury in rats is inflicted on anesthetized animals by immersion of the area to be burned into a water bath. The area of exposure is determined by the aperture in the template, which can be an elaborately designed apparatus, 1 a metal wire cage, 2 or a simple metal plate with a rectangular aperture 3 through which the body part is immersed in a water bath for a specified amount of time.To establish a standard long-term method for protecting the burn wound dressings from being eaten by the rats and thereby tainting the scientific results, we searched the relevant literature and found that there was nothing available for that purpose. After several tests, the armor described later used together with our scald template was found to be satisfactory for our experiment series. With the use of the armor, the silver detected in the blood should originate only from the wound, with no additional absorption from the gut.In this article, an innovative scald protocol and armor are being presented. With this template firmly pressed on the shaved back, only the area to be burned is in contact with the boiling water. The burn wound is uniform in size and depth and can certainly be reproduced. The template is easy and fast to fabricate at a low cost and at any size. The armor ensures that the dressings stay in place for a minimum of four or even more weeks and prevents the rats from eating the silver-coated wound material. MATERIALS AND METHODS Experimental Scald ModelTen female and 4 male Sprague-Dawley rats (Himberg, Austria) weighing 250 g to 350 g were allowed to acclimatize for 1 week before use. The animals had free access to water and standard rat chow with intermittent cycles of light and dark in a temperature-controlled environment at The experiments were performed in accordance with European and Austrian laws on animal experimentation. All animals were weighed and then preanaesthetized in an anesthesia-induction box with an isoflurane-oxygen mix (0Y4% isoflurane vaporizer setting in 100% oxygen, stepwise increase; oxygen flow, 1.5 L/min.). Once asleep, midazolam (2 mg/kg), medetomidine (0.15 mg/kg) and fentanyl (5 Kg/kg) were administered subcutaneously, and the eyes were protected with oleovit-A eye ointment. The full anesthetic effect was achieved after 5 minutes, and the scalding procedure was started when there was no more toe pinch reflex. Thereafter, the back of each rat was carefully shaved with an electric razor. In the prone position, the animal was placed onto a soft cylindrical bolster, and the flexible template was closely pressed onto the rat's back. One person held the rat and the bolster, the second held the template, and the third pou...

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Section: Discussionmentioning

confidence: 99%

Innovative scald burn model and long-term dressing protector for studies in rats

Pfurtscheller

Petnehazy²,

Goessler³

et al. 2013

Journal of Trauma and Acute Care Surgery

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show abstract

“…Group B injury establishes a partial thickness burn as proved by Besner et al [11]. Groups C and D burn injuries can be considered as full thickness burns by Carter et al [12].…”

Section: Experimental Designmentioning

confidence: 96%

Immune responses in relation to the type and time of thermal injury: An experimental study

Alexis

Carrer

Droggiti

et al. 2015

Injury

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“…Anesthesia was achieved with pentobarbital sodium solution at a dose of 50 mg/kg by intra-peritoneal injection (Abbott Laboratories, USA). Both excisional wound model and burn wound model have been described previously [23,24]. In brief, a full-thickness piece of skin measuring 1.0 × 1.0 cm 2 was excised on the back of each mouse using a pair of scissors for the excisional wound model.…”

Section: Animals and Burn Wound Modelmentioning

confidence: 99%

Dendrimer encapsulation enhances anti-inflammatory efficacy of silver nanoparticles

Liu

Hao

Lok

et al. 2014

Journal of Pediatric Surgery

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A Standardized Model of Partial Thickness Scald Burns in Mice

Cited by 42 publications

References 21 publications

Innovative scald burn model and long-term dressing protector for studies in rats

Innovative scald burn model and long-term dressing protector for studies in rats

Immune responses in relation to the type and time of thermal injury: An experimental study

Dendrimer encapsulation enhances anti-inflammatory efficacy of silver nanoparticles

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