A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene

Abstract

Standard procedures regarding osteoblast cells deposition on different subtrates to test the biocompatibility of materials are not available. Aspects related to cells morphology depending on their number on the surface area of the substrate were studied for the first time. Osteoblasts were successfully isolated from residual bone resulted from two different patients after total hip arthroplasty. Different numbers of osteoblasts in populations (3x104 cells/cm2, 6x104 cells/cm2, 1x105 cells/cm2) were deposited on tissue culture polystyrene for 7 and 10 days of incubation. Results suggested that the protocol of osteoblasts deposition should be adjusted to assure a fair distribution of cells on the entire available area of the substrate, possible if a very good dispersion in the medium is achieved prior to deposition. Some other key factors in cells appropriate development, such as substrate nature, topography and incubation time,were detected. The present investigation helps declaring specific standards with respect to the biocompatibility testing of materials using osteoblast populations. Further investigations may lead to the creation of databases and the establishment of standards regarding bicompatibility researches that involve osteoblasts populations.

Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback

“…Some conclusions have been drawn previously related to cells seeding on 2D substrates. Cells number shall be adjusted depending on both the available area of the substrate and its nature [41,42]. A larger number of cells in the fluid as predicted through modelling will assure a higher number of arrested cells in the scaffold.…”

Primary MSCs for Personalized Medicine: Ethical Challenges, Isolation and Biocompatibility Evaluation of 3D Electrospun and Printed Scaffolds

“…Cells populations containing ~6 × 10 4 osteoblasts were seeded on each tested substrate. This number of cells has been selected based on a previous study where the seeding procedure is also described [ 46 ]. Cells were stained with Osteocalcin Monoclonal Antibody (Invitrogen OC4–30, Waltham, MA, USA) and ALPL Recombinant Rabbit Monoclonal Antibody (Invitrogen 7H11L3, Waltham, MA, USA) with Alexa Fluor 488 Goat Anti-Rabbit IgG (Invitrogen H+L, Waltham, MA, USA) and Alexa Fluor 594 Goat Anti-Mouse IgG (Invitrogen H+L, Waltham, MA, USA) as secondary antibodies, to observe their osteogenic differentiation.…”

Gradient 3D Printed PLA Scaffolds on Biomedical Titanium: Mechanical Evaluation and Biocompatibility

“…A cell population containing ~6 × 10 4 osteoblasts were seeded on each testing substrate. This number of cells has been considered the most appropriate for the type of materials involved in the present research and was selected according to the output of a previous study [ 21 ].…”