A study of cytotoxicity and proliferation of Cosmos caudatus Kunth leaf extract in human gingival fibroblast culture

Abstract

Background: Post-extraction dental sockets clinically resolve within a period of 3-4 weeks. However, complete healing and bundling of gingival fibers may require several months. Medication is therefore required to accelerate the healing process. Cosmos caudatus (C. caudatus), a local plant with antioxidant properties and high calcium content, has the potential to promote wound healing while also reportedly capable of strengthening bone. Previous studies have demonstrated the effectiveness of C. caudatus as an alternative treatment for post-menopausal osteoporosis by investigating the dynamic and cellular parameters of bone histomorphometry. Purpose: The study aimed to examine the citotoxicity and proliferation of human gingival fibroblast cells culture after the application of C. caudatus extract. Methods: Cultures of human gingival fibroblast cells with 5x104 cell density were divided into two groups and placed in a 30-well culture dish. The control group contained human gingival fibroblast cell culture without extract, while the experimental group consisted of human gingival fibroblast cells culture with extract. The concentrations of extract were 1200 μg/ml, 600 μg/ml, 300 μg/ml, 150 μg/ml, and 75 μg/ml. A toxicity test was conducted and the optimum concentration evaluated using an MTT assay, while fibroblast numbers on were calculated days 1 and 2 by means of a hemocytometer. Research data was analyzed using a one-way ANOVA test. Results: No toxicity was found. The optimum concentration was 600 μg/ml and fibroblast proliferation was significantly higher in the experimental group compared to the control group, p=0.002 (P<0.05). Conclusion: C. caudatus leaf extract is non-toxic and increases the proliferation of human gingival fibroblast culture at an optimum concentration of 600 μg/ml.

Inhibitory effect of lemongrass extract (Cymbopogon citratus) in supragingival plaque bacterial growth for gingivitis patient: A research study

“…However, that does not mean that gram-negative bacteria's growth in supragingival plaques cannot be inhibited, only in more significant duration and concentration. [24][25][26] The cell viability test, which is a test to measure cell life's ability by calculating the number of living cells, supports the inhibitory impact of lemongrass extract against supragingival plaque bacteria in gingivitis patients. It is exposed to the material and to be tested by Methyl Thiazolyl Tetrazolium (MTT) assay method read using Enzyme-Linked Immunoassay (ELISA) Reader absorbent.…”

Local knowledge of plant-based nutrition sources from forgotten foods in Datengan Village, East Java, Indonesia

“…Cosmos caudatus leaf extract shows impacting SGPT levels in the blood of Wistar rats-induced paracetamol (Maulida et al 2020). C. caudatus leaf extract at a 600 μg/ml concentration increased the proliferation of human gingival fibroblasts culture and is non-toxic activity (Shabrina et al 2018). Susanto et al (2020) stated that C. caudatus at a maximum dose of 7.5 kGy could be preserved using gamma irradiation to avoid changing its anti-cancer properties.…”

Cytotoxic Activity Against L1210 Leukemia Cells from the Ethyl Acetate Fraction of Kenikir Leaves (Cosmos. Caudatus) Preserved by Gamma Irradiation

“…Indonesian people is kenikir (Cosmos caudatus). C. caudatus has properties healing high blood pressure, anti-diabetes, anti-inflammatory, anti-bacterial and anticancer [3]. The secondary metabolite of C. caudatus leaf extract consists of flavonoids, especially quercitrin glycosides.…”