SummaryBackgroundThe aim of the study was to determine the root canal morphology of permanent mandibular incisor teeth in the Indian subpopulation with the use of cone beam computed tomography (CBCT).Material/MethodsCBCT images of 200 patients with 800 permanent mandibular incisors, fulfilling necessary inclusion criteria and aged 18 to 60 years were evaluated. The number of roots, number of root canals and canal configuration were investigated and then classified according to Vertucci’s classification of root canals. The effect of gender on the incidence of root canal morphology was also investigated.ResultsAll the permanent mandibular incisors had a single root. The majority of mandibular incisors (66.5%) had a single root with a single canal. The prevalence of second canals was as follows: right central incisor – 33.5%, left central incisor – 30%, right lateral incisors – 33.5% and left lateral incisor – 36.5%. According to gender, 15.2% of men and 20.4% of women had a second root canal. Type 1 Vertucci configuration was most prevalent, followed by type 3, type 2, type 5 and type 4 in that order.ConclusionsType 1 Vertucci’s classification (64.5%) was the most prevalent canal configuration in the mandibular anterior teeth in the Indian population. Type 5 Vertucci’s classification was the most frequently observed canal configuration of the two-canalled teeth. CBCT is an excellent imaging modality for detection of different canal configurations of mandibular incisors.