A Study of Virulence Factors and Drug Resistance Pattern in Escherichia coli Isolated from Extra Intestinal Infections in a Tertiary Care Teaching Hospital, Chinakakani, Guntur, Andhra Pradesh, South India

Bhrugubalda, Anuradha; Penmetcha, Uma; Yarlagadda, Padmaja; Myeneni, Ramesh Babu

doi:10.20546/ijcmas.2016.504.019

Cited by 3 publications

(7 citation statements)

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“…Serum resistance in UPEC strains has been typically associated with pyelonephritis, cystitis and bacteraemia. 7 In the current study, all 75 isolates showed resistance to serum bactericidal action. This is similar to the studies by Sharma et al and Shetty SK et al which reported 86.7% and 83% of serum resistance.…”

Section: Discussionmentioning

confidence: 51%

“… 10 , 18 However, study by Anuradha et al revealed 51% of serum resistance. 7 In UPEC, capsule and the O antigen are polysaccharides that contribute to virulence. The extracellular polysaccharides are antiphagocytic and inhibit complement-mediated lysis.…”

Section: Discussionmentioning

confidence: 99%

“…As a result, the therapeutic options available for the treatment of UTI are cut down which in turn is linked to treatment failure and increase in the economic burden of the community. 7 …”

Section: Introductionmentioning

confidence: 99%

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Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center

Bhat

et al. 2022

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Background: Urinary tract infection(UTI) is one of the commonly prevalent bacterial infection in humans.The uropathogenic E. coli (UPEC) expresses a range of virulence factors that contribute to their pathogenicity. The emergence of multidrug resistance (MDR)-associated UTI is increasing.This study monitors the distribution of virulence factors among UPEC strains to note the antibiogram, outcome and type of associated UTI. Methods:A prospective cross-sectional time-bound study of six months was done on clinically significant urinary isolates of Escherichia coli. Detection of haemolysin production and serum resistance was done by phenotypic methods. Genotypic characterization of the virulence genes (papC, iutA, hlyA, cnf1) was done by multiplex PCR. Demographic data, clinical history, antibiogram and type of UTI was collected from clinical case records. Results:75 E.coli isolates from patients with suspected UTIs were included. Females had a higher preponderance of UTI (66.7%). 93% of patients were adults and the remaining 7% were from paediatrics. 24 (32%) isolates showed haemolysis by plate haemolysis and all isolates were serum-resistant. Out of 75 isolates, 65 were positive for at least one of four targeted genes, while remaining ten isolates were negative for all four genes.Multidrug resistance was found in 40 (53.3%) isolates. 97.4% of the UTI cases had a favourable clinical outcome at discharge. Mortality due to urosepsis was 2.6%. Conclusion:Association of hemolysin production with resistance to imipenem and norfloxacin in UPEC strains was significant.Presence of hlyA gene is positively associated with ceftazidime resistance. Nitrofurantoin, piperacillin, tazobactam, and cefaperazone sulbactam are possible candidates for empirical therapy of UTIs. Drugs like aminoglycosides, carbapenems and fosfomycin may be used as reserve drugs in the treatment of MDR-UTI.However, inappropriate usage can increase antibiotic resistance. Hence proper selection of antibiotics in hospitals taking into account the local antibiogram is needed to reduce the emergence of antibiotic resistance.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

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Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center

Bhat

et al. 2022

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“…and high vaginal swab (0.7%) samples. In the study by Bhrulgubalda et al, the clinical samples distribution was as follows: urine (92%), pus (5%), sputum (1%), ascetic fluid (1%), and blood (1%) (22). Similarly, in the study conducted by Fakruddin Md et al, 65 clinically isolated E. coli were studied in which most of them i.e 35% were from urine, 18.46% from peritoneal, 18.46% from blood, 15.38% from pus and 9.23% from CSF (23).…”

Section: Discussionmentioning

confidence: 99%

Virulence And Drug Resistance Pattern Of Escherichia Coli Isolates From Various Clinical Sample

Baral

2023

J. Manmohan Memorial Inst. Health. Sci.

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Introduction: E. coli is one of the most common pathogens, and its infection has resulted in a global burden due to its increased medication resistance and virulence factors. The goal of this study was to examine the drug resistance pattern and pathogenicity properties of Escherichia coli isolates. Methods: Over the course of six months (March 2019-August 2019), a laboratory-based cross-sectional study was done among patients visiting Manmohan Memorial Teaching Hospital in Kathmandu, Nepal. Standard microbiological procedures were used to identify bacterial isolates from clinical specimens. The modified Kirby Bauer disk diffusion method was used to determine antibiotic susceptibilities. The combined disk test method was used to confirm the ESBL and MBL. To determine their virulence, serum bactericidal activity and biofilm productions were determined. Results: 59.60 percent (n=81) of the 136 Escherichia coli isolates were multidrug resistant, 25.70 percent (n=35) were ESBL producers, and 11.80 percent (n=16) were MBL producers. Serum resistance was discovered in 22.8 percent (n=31) of the total isolates, while biofilm formation was found in 19.11 percent (n=26). Amoxycillin had the highest level of resistance (87.5%), whereas Chloramphenicol (93.4%) and Imipenem (80.9%) were the most susceptible antibiotics. Polymyxin B and Colistin sulphate were absolutely sensitive. In our investigation, 61.5 percent of biofilm producers were MDR, with non-beta lactamase types being the most common. MBL producers were discovered to be more serum resistant. Amikacin and Imipenem were found to be more sensitive to biofilm makers. Conclusions: The expression of Escherichia coli virulence factors varied depending on the kind of infection. Escherichia coli has a high rate of multidrug resistance. To minimize the emergence and spread of antibiotic resistance in bacteria, proper identification of drug-resistant bacteria, careful use of antibiotics, and effective antibiotic policy are required.

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“…9,17 However, study by Anuradha et al revealed 51% of serum resistance. 7 In UPEC, capsule and the O antigen are polysaccharides that contribute to virulence. The extracellular polysaccharides are antiphagocytic and inhibit complement-mediated lysis.…”

Section: Discussionmentioning

confidence: 99%

Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center

Bhat²,

K³

et al. 2022

F1000Res

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Background: Urinary tract infections (UTI) are the most prevalent bacterial infection in humans. The uropathogenic E. coli (UPEC) expresses a range of virulence factors that contribute to their pathogenicity. The emergence of multidrug resistance (MDR)-associated UTI is increasing. This study monitors the distribution of virulence factors among UPEC strains to note the antibiogram, outcome and type of associated UTI. Methods: A prospective cross-sectional time-bound study of six months was done on clinically significant urinary isolates of Escherichia coli. Detection of haemolysin production and serum resistance was done by phenotypic methods. Genotypic characterization of the virulence genes (papC, iutA, hlyA, cnf1) was done by multiplex PCR. Demographic data, clinical history, antibiogram and type of UTI was collected from clinical case records. Results:75 E.coli isolates from patients with suspected UTIs were included. Females had a higher preponderance of UTI (66.7%). 93% of patients were adults and the remaining 7% were from paediatrics. 24 (32%) isolates showed haemolysis by plate haemolysis and all isolates were serum-resistant. Out of 75 isolates, 65 were positive for at least one of four targeted genes, while remaining ten isolates were negative for all four genes. Multidrug resistance was found in 40 (53.3%) isolates. 97.4% of the UTI cases had a favourable clinical outcome at discharge. Mortality due to urosepsis was 2.6%. Conclusion: Association of hemolysin production with resistance to imipenem and norfloxacin in UPEC strains was significant. Presence of hlyA gene is positively associated with ceftazidime resistance. Nitrofurantoin, piperacillin, tazobactam, and cefaperazone sulbactam are possible candidates for empirical therapy of UTIs. Drugs like aminoglycosides, carbapenems and fosfomycin may be used as reserve drugs in the treatment of MDR-UTI. However, inappropriate usage can increase antibiotic resistance. Hence proper selection of antibiotics in hospitals taking into account the local antibiogram is needed to reduce the emergence of antibiotic resistance.

show abstract

A Study of Virulence Factors and Drug Resistance Pattern in Escherichia coli Isolated from Extra Intestinal Infections in a Tertiary Care Teaching Hospital, Chinakakani, Guntur, Andhra Pradesh, South India

Cited by 3 publications

References 31 publications

Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center

Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center

Virulence And Drug Resistance Pattern Of Escherichia Coli Isolates From Various Clinical Sample

Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center

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