A Study on Lipid Peroxide-induced Lens Damage In Vitro

Mibu, Hiroyuki; Nagata, Masanobu; Hikida, Mitsushi

doi:10.1006/exer.1994.1197

Cited by 24 publications

(13 citation statements)

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“…11) Several studies have explored the mechanisms of cataract development over the past several decades. 12) Oxidative stress such as by radical oxygen species, 12) hydrogen peroxide, 3) and lipid peroxides 13) is considered important in perturbing lens homeostasis. However, due to uncertainties regarding efficacy and safety, a potent anticataract drug for human cataracts has not yet been introduced.…”

mentioning

confidence: 99%

Inhibitors of Inducible Nitric Oxide Synthase Prevent Damage to Human Lens Epithelial Cells Induced by Interferon-Gamma and Lipopolysaccharide

Nagai

Liu

Fukuhata

et al. 2006

Biological & Pharmaceutical Bulletin

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mentioning

confidence: 99%

Inhibitors of Inducible Nitric Oxide Synthase Prevent Damage to Human Lens Epithelial Cells Induced by Interferon-Gamma and Lipopolysaccharide

Nagai

Liu

Fukuhata

et al. 2006

Biological & Pharmaceutical Bulletin

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“…Hydrogen peroxide (H202) is a natural oxidant present in tissues of the anterior segment of the eye, such as the comea, iris-ciliary body and lens [5][6][7]. Intracamerally administered H202 has been shown to lower intraocular pressure and to cause significant morphological changes in the pigment epithelium, blood vessels and ciliary processes of mature and immature rabbits [5,8].…”

Section: Introductionmentioning

confidence: 99%

Role of Cyclic AMP in Hydrogen Peroxide-Induced Potentiation of Sympathetic Neurotransmission in the Bovine Iris

Opere¹,

Ohia²

1997

Journal of Ocular Pharmacology and Therapeutics

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Hydrogen peroxide (H2O2) has been shown to enhance electrically-evoked norepinephrine (NE) release from isolated, superfused bovine irides. Since stimulation of presynaptic adenylyl cyclase can potentiate sympathetic neurotransmission in several tissues, the present study considered the possibility that cyclic AMP may mediate the effects of H2O2 in the iris. Isolated bovine irides were prepared for analysis of field stimulation-induced [3H]NE release using the superfusion method. Both the diterpene activator of adenylyl cyclase, forskolin and the cyclic AMP-specific phosphodiesterase inhibitor, RO-201724 enhanced evoked [3H]NE overflow by 32%. On the other hand, inhibition of cyclic AMP-dependent protein kinase I/II by Rp-cAMPS attenuated field-stimulated [3H]NE release by 20%. Interestingly, both RO-201724 and Rp-cAMPS did not alter the enhancement of electrically-evoked [3H]NE overflow caused by submaximal concentrations of H2O2. We conclude that cyclic AMP may be involved in the pathway leading to NE release from sympathetic nerves in the bovine isolated iris. However, cyclic AMP may not be a mediator of H2O2-induced potentiation of sympathetic neurotransmission in this tissue.

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“…The Mg 2ϩ in the lens increased in diabetic 14) and ultraviolet-irradiated rats. 18) On the other hand, lipid peroxides 19) and cigarette smoke 17) reduced the Mg 2ϩ in the lens. In human senile cataract, Dilsiz et al reported that lens ionic imbalance with increased levels of calcium and sodium, coupled with decreased levels of magnesium and potassium, is related to cataract development.…”

mentioning

confidence: 99%

Delay of Cataract Development in the Shumiya Cataract Rat by the Administration of Drinking Water Containing High Concentration of Magnesium Ion

Nagai

Ito

Inomata

et al. 2006

Biological & Pharmaceutical Bulletin

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Mineral ion, an essential element, plays a very important biochemical role in the body. It has been recognized that mineral ion imbalance is associated with several major diseases, such as diabetes mellitus, hypertension, cardiovascular and cerebrovascular diseases and heart infarction. [1][2][3][4][5][6][7] In addition, Iwata and Kinoshita reported mineral ion imbalance in the lens causes cataract. 8,9) Any alteration in the optical homogeneity of the lens or decrease in its transparency is known as a cataract, 10,11) and numerous factors have been implicated in its etiology. These involve genetic factors, diabetes, smoking, nutrition, the cumulative effect of X-rays, ultraviolet irradiation, and alteration in both endocrine and enzymatic equilibrium. [12][13][14][15][16][17] Cataractous lenses have been found to have an altered distribution of the intracellular ionic environment. The concentration of calcium ion (Ca 2ϩ ) was increased in many cataractous lenses. There have been many reports on the relationship between Mg 2ϩ and cataract. The Mg 2ϩ in the lens increased in diabetic 14) and ultraviolet-irradiated rats. 18) On the other hand, lipid peroxides 19) and cigarette smoke 17) reduced the Mg 2ϩ in the lens. In human senile cataract, Dilsiz et al. reported that lens ionic imbalance with increased levels of calcium and sodium, coupled with decreased levels of magnesium and potassium, is related to cataract development. 20)These lens ionic imbalances arise as a result of changes to lens membrane characteristics causing an increase in lens membrane permeability.Over the past several decades, there have been many studies exploring the mechanisms of cataract development. 21)Currently, reactive oxygen species (ROS), induced by UV rays in sunlight, are considered to be important in perturbing lens homeostasis. Therefore, exposure to ROS results in a breakdown of lens homeostasis, and the Ca 2ϩ concentration in the lens becomes elevated. The elevated Ca 2ϩ concentration in the lens has been induced to activate calpain, a Ca 2ϩ -dependent protease. Furthermore, the degradation of lens proteins such as crystallin proteins would result in an opaque lens. 20,22) This ROS is enhanced by hyperglycemia and Mg deficiency. 23)The Shumiya cataract rat (SCR), which was established by Shumiya and Nagase, is a hereditary cataractous rat strain. 24)Lens opacity in SCR appears spontaneously in the perinuclear and nuclear portions at 11-12 weeks of age, and cataract appearance in adult SCR was 66.7%.25) Previous investigations have revealed that oxidized glutathione concentrations in the SCR lens are increased, and reduced glutathione values are decreased.26) The proteolysis of some crystallins and cytoskeletal proteins was significantly enhanced in cataractous SCR lenses. The calcium concentrations in cataractous lenses rise markedly with age compared with non-cataractous lenses, and the autolytic product of calpain is also detected in cataractous lenses.27) It is noteworthy that SCR cataracts are not diabetic cataracts. T...

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A Study on Lipid Peroxide-induced Lens Damage In Vitro

Cited by 24 publications

References 0 publications

Inhibitors of Inducible Nitric Oxide Synthase Prevent Damage to Human Lens Epithelial Cells Induced by Interferon-Gamma and Lipopolysaccharide

Inhibitors of Inducible Nitric Oxide Synthase Prevent Damage to Human Lens Epithelial Cells Induced by Interferon-Gamma and Lipopolysaccharide

Role of Cyclic AMP in Hydrogen Peroxide-Induced Potentiation of Sympathetic Neurotransmission in the Bovine Iris

Delay of Cataract Development in the Shumiya Cataract Rat by the Administration of Drinking Water Containing High Concentration of Magnesium Ion

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