A study on the mechanism of protein adsorption to TiO2

Ellingsen, Jan Eirik

doi:10.1016/0142-9612(91)90057-h

Cited by 314 publications

(199 citation statements)

References 13 publications

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“…These hydroxyl groups may well promote adsorption of calcium-binding extracellular matrix proteins (e.g., alkaline phosphatase) and RGD-specific peptide sequences (e.g., fibronectin, bone sialoprotein). 12,39 Interestingly, in OD-MAPCs loaded TiO 2 -HAPGEL scaffolds, several areas of osteointegration were observed after 8 and 12 weeks. Furthermore, mineralized NFB tissue was seen in the pores and filled the defect length completely (Figs.…”

Section: Discussionmentioning

confidence: 98%

“…10 Titania additives may constitute an advantage by increasing surface hydrophobicity and protein adsorption to further enhance cell adhesion, osteogenic induction, and formability in bone biomaterials, including in HAP-GEL. 7,11,12 Consequently, bone regeneration of formable titanium dioxide (TiO 2 )-enriched HAP-GEL scaffolds was compared in this study to HAP-GEL alone.…”

Section: Introductionmentioning

confidence: 99%

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Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Ferreira

Padilla

Urkasemsin

et al. 2013

Tissue Engineering Part A

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Adequate bony support is the key to re-establish both function and esthetics in the craniofacial region. Autologous bone grafting has been the gold standard for regeneration of problematic large bone defects. However, poor graft availability and donor-site complications have led to alternative bone tissue-engineering approaches combining osteoinductive biomaterials and three-dimensional cell aggregates in scaffolds or constructs. The goal of the present study was to generate novel cell aggregate-loaded macroporous scaffolds combining the osteoinductive properties of titanium dioxide (TiO 2 ) with hydroxyapatite-gelatin nanocomposites (HAP-GEL) for regeneration of craniofacial defects. Here we investigated the in vivo applicability of macroporous (TiO 2 )-enriched HAP-GEL scaffolds with undifferentiated and osteogenically differentiated multipotent adult progenitor cell (MAPC and OD-MAPC, respectively) aggregates for calvaria bone regeneration. The silane-coated HAP-GEL with and without TiO 2 additives were polymerized and molded to produce macroporous scaffolds. Aggregates of the rat MAPC were precultured, loaded into each scaffold, and implanted to rat calvaria criticalsize defects to study bone regeneration. Bone autografts were used as positive controls and a poly(lacticco-glycolic acid) (PLGA) scaffold for comparison purposes. Preimplanted scaffolds and calvaria bone from pig were tested for ultimate compressive strength with an Instron 4411 Ò and for porosity with microcomputerized tomography (mCT). Osteointegration and newly formed bone (NFB) were assessed by mCT and nondecalcified histology, and quantified by calcium fluorescence labeling. Results showed that the macroporous TiO 2 -HAP-GEL scaffold had a comparable strength relative to the natural calvaria bone (13.8 -4.5 MPa and 24.5 -8.3 MPa, respectively). Porosity was 1.52 -0.8 mm and 0.64 -0.4 mm for TiO 2 -HAP-GEL and calvaria bone, respectively. At 8 and 12 weeks postimplantation into rat calvaria defects, greater osteointegration and NFB were significantly present in the TiO 2 -enriched HAP-GEL constructs with OD-MAPCs, compared to the undifferentiated MAPCloaded constructs, cell-free HAP-GEL with and without titanium, and PLGA scaffolds. The tissue-engineered TiO 2 -enriched HAP-GEL constructs with OD-MAPC aggregates present a potential useful therapeutic approach for calvaria bone regeneration.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Ferreira

Padilla

Urkasemsin

et al. 2013

Tissue Engineering Part A

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“…It is hypothesized that titanium acquires, through interaction with the atmosphere, a passive surface layer of TiO 2 that adsorbs Ca 2? ions, which then react with Ca-binding proteins or glycosaminoglycans, such as osteocalcin, osteopontin or heparan sulfate proteoglycan (HSPG) [24]. Indeed, some reports suggest that osteocalcin and osteopontin are key factors in achieving osteoblastic cell adhesion to dental implants [25,26].…”

Section: Discussionmentioning

confidence: 99%

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

et al. 2012

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In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium plates hydrothermally-treated with CaCl2 (Ca) or MgCl2 (Mg) solution, or distilled water (DW). The number and adhesive strengths of attached cells on the substrata were then analyzed. The number of Sa3 cells adhering to the Ca- and Mg-treated plates was significantly larger than in the DW group, but the strength of this adhesion did not differ significantly between groups. In contrast, NIH3T3 cell adhesion number and strength were increased in both the Ca and Mg groups compared to the DW group. Fluorescent microscopic observation indicated that, in all groups, Sa3 had identical expression levels of integrin β4 and development of actin filaments, whereas NIH3T3 cells in the Ca and Mg groups displayed much stronger punctate cytoplasmic signals for vinculin and more bundle-shaped actin filaments than cells in the DW group. As a result, it was indicated that the hydrothermal treatment of titanium with Ca or Mg solution improved the integration of soft tissue cells with the substrata, which may facilitate the development of a soft tissue barrier around the implant.

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“…When exposed to air, Ti reacts immediately with oxygen, forming a surface oxide layer. Accordingly, the TiO2 layer adsorbs ionized Ca 27) . Acidic hydroxyl (OH)b groups that are charged negatively could be directly adsorbed to ionized Ca 28) .…”

Section: Discussionmentioning

confidence: 99%

Initial attachment of human oral keratinocytes cultured on zirconia or titanium

et al. 2012

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Initial attachment of human oral keratinocytes (HOKs) cultured on mirror-surfaced commercially pure titanium (Ti) or yttriastabilized tetragonal zirconia polycrystals (TZP) was investigated. Numbers of viable attached HOKs, their mRNAs and proteins expression of laminin γ2 and integrin β4 were evaluated using the WST-1 assay, quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Localization of laminin γ2 and integrin β4 was observed using immunofluorescent staining. Cell spreading was evaluated by measuring the perimeter of actin on fluorescent stained images, and cell morphology was examined using scanning electron microscopy. At 1 h TZP elicited less of initial attachment than Ti in terms of mRNAs expression and proteins expression of laminin γ2 and integrin β4 (p<0.05). However, at 48 h TZP was showed similar initial attachment in comparison to Ti. Therefore, it was suggested that TZP has a potential to form epithelial attachment like Ti.

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A study on the mechanism of protein adsorption to TiO2

Cited by 314 publications

References 13 publications

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

Initial attachment of human oral keratinocytes cultured on zirconia or titanium

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