A subtropical case of human babesiosis

Olmeda, A. Sonia; Armstrong, PhilipM.; Rosenthal, Benjamin M.; Valladares, Basilio; Castillo, Antonio del; Armas, Flores; Miguélez, Máximo; González, A Murilla; Rodríguez, JM; Spielman, Andrew; Telford, Sam R.

doi:10.1016/s0001-706x(97)00045-4

Cited by 165 publications

(89 citation statements)

References 4 publications

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“…Although the particular species of Babesia was not determined by sequencing, we confirmed that the amplification was specific to Babesia sp. by using two different sets of primers which were described to be specific to the Babesia genus [33,34].…”

Section: Discussionmentioning

confidence: 99%

“…or Babesia sp. DNA in tick DNA extracts was tested by PCR using specific primers for each of the pathogens [32][33][34]39]. The primer sets used in this study and PCR conditions are listed in Table I. DNA electrophoresis was carried out in 2% agarose gels containing ethidium bromide, and DNA fragments were visualised under ultraviolet light.…”

Section: Pcr Amplificationmentioning

confidence: 99%

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Evidence of Bartonella sp. in questing adult and nymphal Ixodes ricinus ticks from France and co-infection with Borrelia burgdorferi sensu lato and Babesia sp.

et al. 2005

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-Ticks are known vectors for a wide range of pathogenic microorganisms. Their role in the transmission of some others is so far only suspected. Ticks can transmit multiple pathogens, however, little is known about the co-existence of these pathogens within questing ticks. We looked for the presence of DNA from three micro-organisms, Bartonella sp., Borrelia burgdorferi sensu lato and Babesia sp. which are known or suspected tick-borne pathogens, using a cohort of 92 questing Ixodes ricinus ticks collected from pastures in northern France. DNA was extracted from each individual tick and the presence of the three pathogens was investigated using Polymerase Chain Reaction (PCR) amplification. Nine among 92 samples (9.8%) demonstrated PCR products using Bartonella specific primers, 3 among 92 (3.3%) using Borrelia burgdorferi sensu lato specific primers and 19 among 92 (20.6%) using Babesia specific primers. Seven among 92 samples (7.6%) were PCR positive for at least two of the pathogens and one sample was positive for all three. Adult ticks (12/18; 67%) showed significantly higher infection rates compared to nymphs (11/74; 15%) for all three pathogens (P < 0.001). This study is the demonstration of the simultaneous presence of Bartonella sp., Borrelia burgdorferi sensu lato and Babesia sp. in questing Ixodes ricinus ticks. questing ticks / co-infection / PCR detection

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Section: Discussionmentioning

confidence: 99%

Section: Pcr Amplificationmentioning

confidence: 99%

Evidence of Bartonella sp. in questing adult and nymphal Ixodes ricinus ticks from France and co-infection with Borrelia burgdorferi sensu lato and Babesia sp.

et al. 2005

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“…(OLMEDA et al, 1997). The presence of F. tularensis was determined by amplification (primers TUL4-435/TUL4-863) of a gene coding for 17 kDa lipoprotein which is conserved among various strains of F. tularensis (SJÖSTEDT et al, 1997).…”

Section: Methodsmentioning

confidence: 99%

Molecular detection of Babesia canis in Dermacentor reticulatus ticks collected in Slovakia

et al. 2006

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Dermacentor reticulatus ticks are recognized as the most important vectors of Babesia canis, the aetiological agent of canine babesiosis occurring throughout Europe. Vector competence of D. reticulatus for B. canis is well described and experimentally determined; however, by using molecular analysis it was proven so by one recent study in Russia. Herein, the additional molecular evidence of B. canis infection in D. reticulatus ticks collected in Slovakia is provided. Using PCR followed by sequencing of distinctive amplicons we determined the presence of Babesia canis canis in one of 100 tested adult ticks. Two zoonotic pathogens, Francisella tularensis and Coxiella burnetii, were previously isolated from D. reticulatus ticks in Slovakia. In our samples, we detected only the presence of F. tularensis.

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“…To detect babesial parasites, each sample was tested with primers PIRO-A and PIRO-B, which were designed to amplify the 407-, 408-and 435-basepair (bp) fragments of the nss rRNA gene of B. odocoilei, B. divergens and B. microti, respectively [1,7]. The alignment of complete sequences of nss rRNA from different species of babesiae deposited in the GenBank revealed that with this primer set, the 407-bp fragment of B. canis nss rRNA could be amplified as well.…”

Section: Analysis Of Dna Sequencesmentioning

confidence: 99%

Canine babesiosis in Slovenia: Molecular evidence ofBabesia caniscanisandBabesia canis vogeli

et al. 2004

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-Canine babesiosis, caused by intraerythrocytic Babesia spp., is a tick-borne disease of worldwide importance. No information on canine babesiosis has been documented in Slovenia. Therefore, 238 dogs admitted to the Small animal clinic in Ljubljana from the years 2000 to 2002 were tested for the presence of babesial parasites in the blood. Based on clinical, microscopic and molecular investigations, 14 dogs (5.9%) were determined as being infected with babesiae. Clinical signs relating to acute haemolysis, fever, anorexia, depression and haematological abnormalities such as anaemia and thrombocytopenia were noticed in most of the 14 infected dogs. The morphology of the parasites was indicative of Babesia canis infection. Two subspecies were detected, namely B. canis canis (11 dogs, 4.6%) and B. canis vogeli (3 dogs, 1.3%) using PCR and subsequent sequence analysis of portions of nns rRNA gene. In addition, based on nucleotide sequence analysis, the 11 isolates of B. c. canis could be subdivided into three groups, whereas the three B. c. vogeli isolates were genetically identical. The results of this study demonstrate the presence of canine babesiosis due to B. c. canis and B. c. vogeli in Slovenia. B. canis canis / B. canis vogeli / molecular analysis / SloveniaČ

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A subtropical case of human babesiosis

Cited by 165 publications

References 4 publications

Evidence of Bartonella sp. in questing adult and nymphal Ixodes ricinus ticks from France and co-infection with Borrelia burgdorferi sensu lato and Babesia sp.

Evidence of Bartonella sp. in questing adult and nymphal Ixodes ricinus ticks from France and co-infection with Borrelia burgdorferi sensu lato and Babesia sp.

Molecular detection of Babesia canis in Dermacentor reticulatus ticks collected in Slovakia

Canine babesiosis in Slovenia: Molecular evidence ofBabesia caniscanisandBabesia canis vogeli

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