“…Complete characterization of pine oil is not possible as the composition of the material is complex and variable. The infrared spectrum of the Tween 80, following the results of Ren et al [57], showed bands of angular deformation C-C and aliphatic C-H between 500 cm −1 and 1000 cm −1 , a signal referring to the deformation =C-H at 945 cm −1 , a signal of solid intensity characteristic of C-OH of primary alcohol at 1099 cm −1 , a 1460 cm −1 signal referring to me- The infrared spectrum of the Tween 80, following the results of Ren et al [57], showed bands of angular deformation C-C and aliphatic C-H between 500 cm −1 and 1000 cm −1 , a signal referring to the deformation =C-H at 945 cm −1 , a signal of solid intensity characteristic of C-OH of primary alcohol at 1099 cm −1 , a 1460 cm −1 signal referring to methyl, a C=C stretch at 1636 cm −1 , a weak signal at 1734 cm −1 for ester C=O, aliphatic CH sp 3 -s stretch at 2918 cm −1 (asymmetric) and 2859 cm −1 (symmetric), and an OH stretch at 3528 cm −1 (Figure 11). The infrared spectrum of the microemulsion, composed of glycerol, water, Tween 80, and pine oil, showed two signals referring to the C-OH stretch of a primary and secondary alcohol, at 1108 cm −1 and 1039 cm −1 , overlapping bands in the region from 1460 cm −1 to 1207 cm −1 referring to C-OH absorptions, CH2 deformation, methyl, and C=C stretching, a signal in 1649 cm −1 referring to the carbonyl (C=O) of ester, sp 3 -s stretching of aliphatic CH at 2928 cm −1 (asymmetric) and 2884 cm −1 (symmetric), and an OH stretch at 3340 cm −1 (Figure 12).…”