A Surgical Rat Model of Sleeve Gastrectomy with Staple Technique: Long-Term Weight Loss Results

Patrikakos, Panagiotis; Toutouzas, Konstantinos G.; Περρέα, Δέσποινα; Menenakos, Evangelos; Pantopoulou, Alkistis; Thomopoulos, Theodore; Papadopoulos, Stefanos; Bramis, John

doi:10.1007/s11695-009-9965-9

Cited by 34 publications

(28 citation statements)

References 26 publications

(22 reference statements)

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“…The transient reduction in food intake and weight gain observed in our study is consistent with other studies in obese and non-obese rodents where SG surgery was shown to decrease body weight as well as cumulative food intake for up to 2-6 wks. (35,37,80,122). In the current study, IT surgery decreased food intake for 4 weeks and weight gain for 3 weeks.…”

Section: Markers Of Glucose Metabolism In Adipose Tissuesupporting

confidence: 46%

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Effects of sleeve gastrectomy and ileal transposition, alone and in combination, on food intake, body weight, gut hormones, and glucose metabolism in rats

Nausheen

Shah

Pezeshki

et al. 2013

American Journal of Physiology-Endocrinology and Metabolism

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Gastric bypass surgeries for obesity are hypothesized to produce weight loss and improve diabetes by multiple mechanisms including gastric restriction and lower gut stimulation.The surgeries typically involve rearrangement of the fore-gut, hind-gut or both. We compared the effects of foregut (Sleeve Gastrectomy, SG), hindgut (Ileal Transposition; IT), both (SG+IT), or sham manipulations, on food intake, body weight and glucose tolerance in male SD rats. SG, IT and SGIT surgeries produced transient reduction in food intake and weight gain but improvements in glucose tolerance. SG, IT and SGIT surgeries increased plasma GLP-1 concentrations; IT and SGIT increased PYY concentrations. The protein abundance of key markers of glucose metabolism (e.g. GLUT4, AMPK, PKA, IRS-1) in muscle and adipose tissue were increased following SG, IT, or SGIT. Improvement in glycemic control following these surgeries appears to be independent of changes in weight and is likely due to enhanced lower gut stimulation.iii Acknowledgment

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Section: Markers Of Glucose Metabolism In Adipose Tissuesupporting

confidence: 46%

“…In order to minimize chances of leakage, the staple line was reinforced with 6/0 silk (Ethicon, Ontario, Canada) continuous suture on staple line (122). The abdominal wall was closed with running 4/0 silk suture, and the skin was sewed with a 3/0 silk suture.…”

Section: Sleeve Gastrectomymentioning

confidence: 99%

Effects of sleeve gastrectomy and ileal transposition, alone and in combination, on food intake, body weight, gut hormones, and glucose metabolism in rats

Nausheen

Shah

Pezeshki

et al. 2013

American Journal of Physiology-Endocrinology and Metabolism

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“…However, this period is equivalent to 3 human years [19]. Although we could not exclude the potential for weight regain after SG, other investigators have demonstrated that weight loss is maintained at 15 weeks after SG in rodents [20,21]. In human beings, the failure of weight lost after SG has been explained by the dilatation of the stomach.…”

Section: Discussionmentioning

confidence: 88%

Hematological, Biochemical, and Immunological Laboratory and Histomorphological Effects of Sleeve Gastrectomy on Female Rats

et al. 2013

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Restriction of food intake leads to immunologic and histomorphological changes in rats demonstrated by Moriyama. This study is planned to show how sleeve gastrectomy restricts food intake and its effects on biochemical liver function, immunologic and hematologic laboratory parameters, and histomorphological changes in rats. We used sleeve gastrectomy model on seven young adult female rats and compared study group with sham and control groups. Food consumption of rats was measured. All rats were sacrificed on the 50th day, and blood and tissue samples were collected. There was a significant low food intake in sleeve gastrectomy group (p=0.013). No differences were observed on hematologic, biochemical, and immunologic laboratory parameters between groups. Toxicity parameters in liver samples such as cytoplasmic atrophy, single-cell hepatocellular necrosis, and necrotic eosinophilic cells were significantly high in sleeve gastrectomy group (p=0.005). Histomorphological examination of the spleen and kidneys revealed significant changes in sleeve gastrectomy and sham groups compared with controls (p=0.004 and p=0.018, respectively). Although sleeve gastrectomy does not lead to alteration in hematologic, biochemical, and immunologic laboratory parameters, it causes decreased food consumption, which results in toxicological histomorphological changes in rat liver as well as some changes in kidney and spleen samples.

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“…However, this time period is the equivalent of 6 human years [28,29]. While we cannot exclude the potential for weight regain following sleeve gastrectomy, other investigators have demonstrated that weight loss is maintained at a 15-week time point following sleeve gastrectomy in rodents [30,31]. Although we still consider our results as preliminary, they warrant a larger comprehensive study, one which would include more rats in order to evaluate more precisely the role of antioxidant genes in obesity pathogenesis.…”

Section: Discussionmentioning

confidence: 81%

Effects of Borax and Sleeve Gastrectomy on mRNA Expression of Antioxidant Genes in Substantia Nigra Tissue of Obese Rats

Gezgin¹,

Öziç²

2016

J Obes Weight Loss Ther

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Aim: To evaluate the effects of borax and sleeve gastrectomy on mRNA expression of antioxidant genes in substantia nigra tissue of obese rats. Methods:Obese rats were fed with a high-fat diet containing 40% additional fat to the diet. Rats were allocated into four groups in random, which were normal rats (Group I) (n=14), obese rats subjected to SG (Group II) (n=14), obese rats subjected to borax (Group III) (n=14), and obese rats subjected to SG and borax (Group IV) (n=14). Catalase, superoxide dismutase (SOD) and glutathione S-transferase (GST) gene expressions were determined by polymerase chain reaction, real-time polymerase chain reaction (RT-qPCR) and western blotting.Results: When normal rats (Group I), and obese rats subjected to SG (Group II) were compared, a decrease in expressions of catalase, SOD and GST genes was observed in Group II. When obese rats subjected to borax (Group III) were compared with Group I and Group II, a decrease in expressions of catalase, SOD and GST genes was observed in Group III. This phenomenon demonstrates that borax and SG both decrease expressions of catalase, SOD and GST genes. Furthermore, the most significant decrease in expressions of catalase, SOD and GST genes was observed in obese rats subjected to SG and borax (Group IV) when compared to other three study groups. Conclusion:The borax decreases molecular obesity and consequently increases the expressions of Catalase, SOD and GST genes. These data show decrease of Catalase, SOD and GST genes in the substantia nigra tissue of obese rats, consistent with the possibility that these changes may contribute to disease pathogenesis.

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A Surgical Rat Model of Sleeve Gastrectomy with Staple Technique: Long-Term Weight Loss Results

Abstract: We have created a surgical rat model of experimental SG model, enabling the further study of biochemical and hormonal parameters.

Cited by 34 publications

References 26 publications

Effects of sleeve gastrectomy and ileal transposition, alone and in combination, on food intake, body weight, gut hormones, and glucose metabolism in rats

Effects of sleeve gastrectomy and ileal transposition, alone and in combination, on food intake, body weight, gut hormones, and glucose metabolism in rats

Hematological, Biochemical, and Immunological Laboratory and Histomorphological Effects of Sleeve Gastrectomy on Female Rats

Effects of Borax and Sleeve Gastrectomy on mRNA Expression of Antioxidant Genes in Substantia Nigra Tissue of Obese Rats

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