A survey of the bacterial flora of the periodontium in the rice rat

Macdonald, James B.; Socransky, S. S.; Sawyer, Sylvia J.

doi:10.1016/0003-9969(59)90015-9

Cited by 20 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fabricius et al (2) found that the ratio of anaerobes to aerobes increased from 3.9 at 90 days to 11.3 at 1060 days. The diversity of the microbiota within a given tooth has also been found to be quite limited in all systems studied (2,9,15). Taken together, these findings suggest that the rat model is quite comparable to humans and non-human primates, both in terms of the range of microorganisms involved and the selective pressures present that limit the diversity of the infecting microbiota.…”

Section: Discussionmentioning

confidence: 72%

“…Prominent isolates in human studies have included Bacteroides/Prevotella, Forphyromonas, Peptostreptococcus, Streptoeoeeus, Enterococcus, Catnpylobactet; Eusobacterium, Eubacteriwn and Propionibaeterium (4,5,18,26). Few data exist exist concerning the nature of the rat microbiota (9,15), and almost nothing is known about organisms that infect root canals in the rat. As noted above, many of the same organisms are present in high numbers in rat root canals as the periapieal lesions begin to expand rapidly between days 7-15.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Changes in root canal microbiota during the development of rat periapical lesions

Tani‐Ishii

Wang

Tanner³

et al. 1994

Oral Microbiology and Immunology

View full text Add to dashboard Cite

Periapical lesions are reproducibly induced in rats by pulp exposure and infection from the oral cavity. Lesions expand rapidly between day 7 and day 15-20 (active phase), with slowed expansion thereafter. In the present study we characterized the root canal microbiota present during the active phase of lesion development in this system. The mandibular first molars of Sprague-Dawley rats were exposed on day 0. The teeth were extracted after 7 days (n = 10 animals) and 15 days (n = 10), and the microbiota present in roots was isolated and characterized. The number of colonies isolated per tooth was similar on day 7 (1.53 +/- 0.64 colony-forming units x 10(3)) and day 15 (1.49 +/- 0.37 colony-forming units x 10(3)). No colonies were isolated from unexposed control teeth. Anaerobic bacteria increased significantly between day 7 (24.3 +/- 5.7%) and day 15 (47.3 +/- 7.5%), and the proportion of gram-negative organisms increased from day 7 (24.3 +/- 6.1) to day 15 (46.9 +/- 6.8). The predominant bacteria included, on day 7: Streptococcus and Bacteroides species; on day 15: species of Streptococcus, Bacteroides, Prevotella, Neisseria and Peptostreptococcus. Streptococcus oralis, Streptococcus mitis, Streptococcus rattus, Bacteroides pneumosintes and Bacteroides ureolyticus were frequently isolated at both points. Although approximately the same mean number of different species (approximately 3.5) was isolated per tooth on both day 7 and 15, the overall diversity of the isolates increased on day 15.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Changes in root canal microbiota during the development of rat periapical lesions

Tani‐Ishii

Wang

Tanner³

et al. 1994

Oral Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

“…. ,..,,, periodontal sites m nee rals (Macdonald., Microorganisms reseinbiing Actinohacillus ac- Socransky & Sawyer 1959). From non-tinomyceWmcomitans and Haemophilus aphrop-periodontal sites, A. actinomycetemcomitans liilus have been isolated from lesions of has been isolated and implicated as the advanced periodontal destruction, as wel!…”

Section: Introductionmentioning

confidence: 99%

Classification and identification of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by cluster analysis and deoxyribonucleic acid hybridizations

Tanner¹,

Visconti²,

Socransky³

et al. 1982

J of Periodontal Research

View full text Add to dashboard Cite

Fifteen gram‐negative, saccharolytic rods isolated from advanced periodonlal pockeis were compared to type and reference strains of Arlinohacillus actinomycetemcomitans, Haemaphilus aphrophilus, and reference strains of Haemophilus paraphrophilus. Phenotypic features of organisms were used in cluster analysis and multidimensional scaling. Deoxyribonucleic acid (DNA) was extracted from all strains and moles percent guanine plus cytosine content determined. DNA/DNA homology experiments were performed between reference strains of each species and oral isolates. Five strains from periodontosis lesions, two strains from Papillon‐Lefevre periodontal lesions, and four strains from periodontitis pockeis of adults were similar to A. actinomycetemcomitans by cluster analyses and by DNA/DNA homologies. Except for a single H. paraphrophilus isolate, the H. aphrophilus and H, paraphrophilus isolates could not be distinguished as two distinct species as there was overlap in biochemical characteristics and DNA/DNA homology. Two strains isolated from periodontosis lesions, two strains from adult periodontilis sites, and three oral reference strains were very similar to each other and to the H. aphrtiphilus type strain using phenotypic features. Hydrogen peroxide decomposition was not found to be a consistent feature of A. actinomycetemcumitans strains isolated from periodontal pockets.

show abstract

“…Our results are in agreement with those of Beighton [1977] who reported a low re covery of the Fusobacterium and Bacteroides species in Sprague-Dawley rats. Macdonald et al [1959] also recovered lower numbers of these species from the rice rat than from man.…”

Section: Discussionmentioning

confidence: 99%

“…The present investigation was an attempt to compare the indigenous oral microflora of Sprague-Dawley rats, an animal employ ed frequently in oral disease investiga tions, and man. Numerous cultural studies of rat and human oral cavities have been published [Harrison, 1938;Macdonald et al, 1959;Liljemark and Gibbons, 1971;Huxley, 1972;Howlett, 1976;Beighton et al, 1979;Ooshimaet al, 1980]. Most have involved assessments of target species dur ing the consumption of high-sucrose diets.…”

mentioning

confidence: 99%

Comparison of the Oral Microbial Flora of Caries-Free Humans and Conventional Sprague-Dawley Rats

Mr¹,

Ge²,

Wa³

1984

Caries Res

View full text Add to dashboard Cite

In this study, the bacteria from molar dental plaque and scrapings of the tongue of 12 conventional Sprague-Dawley rats on a diet of Purina Rodent Laboratory Chow No. 5001 and tap water were identified at the species or genus level quantitated using anaerobic culture techniques and selected biochemical tests. These results were compared to the microbial composition of dental plaque and scrapings from dental and tongue sites, evaluated by similar cultural techniques, of 7 caries-free students on a low-sugar diet for 3 weeks. Similarities did exist between the human and oral microflora, which included the percentage of gram-positive cocci, gram-negative cocci, Streptococcus mutans, and Streptococcus sanguis recovered at the dental sires. Differences from the human included the percentage of Actinomyces species, coliforms, enterococci, and Veillonella species recovered from the dental sites. The results suggest that the oral microflora of Sprague-Dawley rats should be considered whe model is used for human oral disease studies.

show abstract

A survey of the bacterial flora of the periodontium in the rice rat

Cited by 20 publications

References 21 publications

Changes in root canal microbiota during the development of rat periapical lesions

Changes in root canal microbiota during the development of rat periapical lesions

Classification and identification of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by cluster analysis and deoxyribonucleic acid hybridizations

Comparison of the Oral Microbial Flora of Caries-Free Humans and Conventional Sprague-Dawley Rats

Contact Info

Product

Resources

About