A system for the detection of chromosomal rearrangements using Sordaria macrospora

Arnaise, Sylvie; Leblon, Gérard; Lares, L.

doi:10.1016/0027-5107(84)90029-0

Cited by 10 publications

(3 citation statements)

References 15 publications

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“…In Sordaria macrospora, a close relative of Neurospora, 73 uv-induced reciprocal translocations were found to have both breakpoints adjacent to centromeres (Arnaise et al 1984). When 23 of these centromere-linked translocations were analyzed in detail, all were shown to undergo frequent 3: 1 segregation (Leblon et al 1986).…”

Section: Three-to-one Segregation In Other Organismsmentioning

confidence: 97%

Three-to-one segregation from reciprocal translocation quadrivalents inNeurosporaand its bearing on the interpretation of spore-abortion patterns in unordered asci

Perkins

Raju²

1995

Genome

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In Neurospora, viable ascospores become black (B) when mature, whereas ascospores that are deficient for a chromosome segment are inviable and usually fail to blacken. The presence of a chromosome rearrangement can be recognized and the type of rearrangement can usually be inferred by visual inspection of asci. When a cross is heterozygous for a reciprocal translocation, asci with eight black ascospores (8B:0W) and asci with eight abortive unpigmented ("white" (W)) ascospores (0B:8W) are theoretically produced in equal numbers if homologous centromeres are equally likely to segregate from the quadrivalent in alternate or adjacent modes. In addition, 4B:4W asci are produced with a frequency characteristic of each reciprocal translocation. Information on ascospore-abortion patterns in Neurospora crassa has come predominantly from unordered ascospore octads ejected from the perithecium. Unordered asci of the 4B:4W type were initially presumed to originate by interstitial crossing over in a centromere-breakpoint interval and their frequency was used as a predictor of centromere locations. However, 4B:4W asci can result not only from interstitial crossing over but also from nondisjunction of centromeres at the first meiotic division, which leads to 3:1 segregation. Ordered linear 4B:4W asci retain the sequence information necessary for distinguishing one mode of origin from the other but unordered asci do not. Crossing over results in one abortive duplication-deficiency ascospore pair in each opposite half of a linear ascus, while 3:1 segregation places both abortive ascospore pairs together, either in the distal half or the basal half of the ascus. In the present study, perithecia were opened and intact linear asci were examined in crosses heterozygous for a varied sample of translocations. Three-to-one segregation rather than interstitial crossing over is apparently the main cause of 4B:4W asci when breakpoints are near centromeres, whereas crossing over is responsible for most or all 4B:4W asci when breakpoints are far-distal. Three-to-one segregation does not impair the usefulness of ejected unordered asci for detecting chromosome rearrangements. Ejected octads are superior to ordered linear asci for distinguishing one type of rearrangement from another, because ascus ejection from the perithecium does not occur until viable ascospores are fully pigmented, enabling true 0B:8W asci to be distinguished from those with eight immature ascospores.

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Section: Three-to-one Segregation In Other Organismsmentioning

confidence: 97%

Three-to-one segregation from reciprocal translocation quadrivalents inNeurosporaand its bearing on the interpretation of spore-abortion patterns in unordered asci

Perkins

Raju²

1995

Genome

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“…Theoretically, sexual fertility can be manifested at various stages from the initiation of perithecia to the formation of ascospores. In filamentous fungi, genetic factors such as spore killers (Turner et al 1987;Nauta & Hoekstra 1993), chromosome rearrangements (Perkins 1974;Arnaise et al 1984) and karyotypic difference between the crossing partners (Pukkila 1992;Poggeler et al 2000) are known to cause decreased fertility at the level of ascospore formation. In this study, ascospores were formed in most mature perithecia in the crosses with CDC-containing strains, suggesting that PDA1-CDC does not hinder the events after fertilization.…”

Section: Effects Of Pda1-cdc On Sexual Fertilitymentioning

confidence: 99%

Meiotic inheritance of a fungal supernumerary chromosome and its effect on sexual fertility in Nectria haematococca

Garmaroodi

Taga

2015

Fungal Biology

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“…In these cases hybrid perithecia with a reduced number of ascospores were formed, most of which were inviable. Low fertility and dead ascospores in crosses of ®lamentous fungi may be correlated with spore killers (Turner et al, 1987;Nauta & Hoekstra, 1993), or may be the result of the segregation of chromosome rearrangements (Perkins, 1974;Arnaise et al, 1984). In the latter case, progeny of crosses between individuals with different karyotypes are often expected to be inviable due to deletions or duplications.…”

Section: Introductionmentioning

confidence: 99%

Karyotype polymorphism correlates with intraspecific infertility in the homothallic ascomycete Sordaria macrospora

Pöggeler¹,

Masloff²,

Jacobsen³

et al. 2000

Journal of Evolutionary Biology

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The homothallic fungus Sordaria macrospora produces perithecia with meiotically derived ascospores. In most cases, intraspecies crosses between strains from different culture collections generate fertile hybrid perithecia in the contact zone of two mycelia. However, in some of these crosses we observed a significant decrease in the fertility of the hybrid perithecia when strains of different origin were used for mating. Since we assumed that chromosome variability between the culture collection strains might contribute to this reduction in fertility, we performed pulsed‐field gel electrophoresis. In the course of our study, we were able to identify two major groups of electrophoretic karyotypes in S. macrospora culture collection strains. A quantitative analysis revealed that polymorphic karyotypes contribute to a reduction of fertility in forced crosses between strains carrying differently sized chromosomes. The observed intraspecific chromosome length polymorphism might have consequences on the speciation process of a homothallic fungus capable of sexual but not of asexual spore formation.

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A system for the detection of chromosomal rearrangements using Sordaria macrospora

Cited by 10 publications

References 15 publications

Three-to-one segregation from reciprocal translocation quadrivalents inNeurosporaand its bearing on the interpretation of spore-abortion patterns in unordered asci

Three-to-one segregation from reciprocal translocation quadrivalents inNeurosporaand its bearing on the interpretation of spore-abortion patterns in unordered asci

Meiotic inheritance of a fungal supernumerary chromosome and its effect on sexual fertility in Nectria haematococca

Karyotype polymorphism correlates with intraspecific infertility in the homothallic ascomycete Sordaria macrospora

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