2012
DOI: 10.1105/tpc.112.102574
|View full text |Cite
|
Sign up to set email alerts
|

A Systems Biology View of Responses to Lignin Biosynthesis Perturbations inArabidopsis 

Abstract: Lignin engineering is an attractive strategy to improve lignocellulosic biomass quality for processing to biofuels and other bio-based products. However, lignin engineering also results in profound metabolic consequences in the plant. We used a systems biology approach to study the plant's response to lignin perturbations. To this end, inflorescence stems of 20 Arabidopsis thaliana mutants, each mutated in a single gene of the lignin biosynthetic pathway (phenylalanine ammonia-lyase1, and cinnamyl alcohol dehy… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

9
359
2
3

Year Published

2015
2015
2020
2020

Publication Types

Select...
5
1

Relationship

1
5

Authors

Journals

citations
Cited by 332 publications
(373 citation statements)
references
References 111 publications
(156 reference statements)
9
359
2
3
Order By: Relevance
“…Earlier metabolite profiling studies on Arabidopsis stems and leaves revealed the presence of a large series of (neo)lignan/oligolignol hexosides (Morreel et al, 2004;Vanholme et al, 2012b). To investigate (1) whether the leaf (neo)lignan/oligolignols are localized in the vacuole and (2) whether their structures are in agreement with those expected from combinatorial monolignol radical coupling, Arabidopsis leaf vacuoles were prepared for subsequent phenolic profiling.…”
Section: Leaf Vacuoles Contain a Plethora Of Hexosides And Malate Estmentioning
confidence: 99%
See 4 more Smart Citations
“…Earlier metabolite profiling studies on Arabidopsis stems and leaves revealed the presence of a large series of (neo)lignan/oligolignol hexosides (Morreel et al, 2004;Vanholme et al, 2012b). To investigate (1) whether the leaf (neo)lignan/oligolignols are localized in the vacuole and (2) whether their structures are in agreement with those expected from combinatorial monolignol radical coupling, Arabidopsis leaf vacuoles were prepared for subsequent phenolic profiling.…”
Section: Leaf Vacuoles Contain a Plethora Of Hexosides And Malate Estmentioning
confidence: 99%
“…To verify this, we profiled Arabidopsis comt mutants, defective in COMT, which catalyzes the methylation of 5-hydroxyferulic acid and 5-hydroxyconiferaldehyde into sinapic acid and sinapyl alcohol, respectively. Lignin in comt mutants is characterized by benzodioxane units formed by the incorporation of 5-hydroxyconiferyl alcohol into the lignin polymer (Vanholme et al, 2012b). Comparative vacuolar profiling of wild-type Arabidopsis and comt mutants (Supplemental Figure 5) revealed the appearance of multiple isomers of the benzodioxane oligolignol hexoside G(8-O-4)5HFA hex, the aglycone that is made through the combinatorial coupling of the COMT substrate 5-hydroxyferulic Table 1 and the footnote of Table 2, respectively.…”
Section: Perturbation Of Monolignol Biosynthesis Affects the Vacuolarmentioning
confidence: 99%
See 3 more Smart Citations