A Thermodynamic Study on the Binding of Cobalt(II) and Iron(III) Ions with Bovine Carbonic Anhydrase II at Different Temperatures

Behbehani, G. Rezaei; Divsalar, Adeleh; Saboury, Ali Akbar; Hajian, Reza; Rezaei, Zahra; Yahaghi, E.; Barzegar, L.

doi:10.1007/s10953-010-9572-3

Cited by 4 publications

(5 citation statements)

References 12 publications

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“…Protein interactions with small ligands (including co‐factors and drugs) Protein/peptide interactions with metals and ions Protein/peptide interactions with nucleic acids …”

Section: Introductionmentioning

confidence: 99%

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

Ghai

Falconer

Collins

2011

J of Molecular Recognition

167

108

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Isothermal titration calorimetry (ITC) is a biophysical technique for measuring the formation and dissociation of molecular complexes and has become an invaluable tool in many branches of science from cell biology to food chemistry. By measuring the heat absorbed or released during bond formation, ITC provides accurate, rapid, and label-free measurement of the thermodynamics of molecular interactions. In this review, we survey the recent literature reporting the use of ITC and have highlighted a number of interesting studies that provide a flavour of the diverse systems to which ITC can be applied. These include measurements of protein-protein and protein-membrane interactions required for macromolecular assembly, analysis of enzyme kinetics, experimental validation of molecular dynamics simulations, and even in manufacturing applications such as food science. Some highlights include studies of the biological complex formed by Staphylococcus aureus enterotoxin C3 and the murine T-cell receptor, the mechanism of membrane association of the Parkinson's disease-associated protein α-synuclein, and the role of non-specific tannin-protein interactions in the quality of different beverages. Recent developments in automation are overcoming limitations on throughput imposed by previous manual procedures and promise to greatly extend usefulness of ITC in the future. We also attempt to impart some practical advice for getting the most out of ITC data for those researchers less familiar with the method.

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“…Protein interactions with small ligands (including co‐factors and drugs) Protein/peptide interactions with metals and ions Protein/peptide interactions with nucleic acids …”

Section: Introductionmentioning

confidence: 99%

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

Ghai

Falconer

Collins

2011

J of Molecular Recognition

167

108

View full text Add to dashboard Cite

show abstract

“…We have shown previously [4][5][6][7][8] that the heats of the macromolecules+ligands interactions in the aqueous solvent systems can be reproduced by the extended solvation model as follows:…”

Section: Resultsmentioning

confidence: 99%

“…There are two distinct sets of binding sites on lysozyme, which are clear in Figure 1. The dissociation equilibrium constant (K d ) and the number of binding sites "g" can be determined by the following equation [4][5][6][7][8] 0 0 The binding parameters recovered from Equations. 1, 5 and 6 were listed in Table 2.…”

Section: Resultsmentioning

confidence: 99%

Calorimetric Study on the Binding of Lysozyme Upon Interaction With β-Cyclodextrin

Behbehani¹,

Taherkhani²,

Saboury³

et al. 2010

J Thermodyn Catal

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Effects of β-cyclodextrin, βCD, on refolding of lysozyme was investigated at pH 12 employing isothermal titration calorimetry (ITC) at 300K in 30mM Tris buffer solution. βCD was employed as an anti-aggregation agent and the heats obtained for lysozyme+βCD interactions are reported and analyzed in terms of the extended solvation model. It was indicated that there are two sets of identical and non-cooperative sites for βCD.

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“…Interestingly, when Fe 3+ replaces the native Zn 2+ , it significantly enhances enzyme activity by modifying the enzymes by metal ion replacement. The complexes formed by Fe 3+ and CA also exhibit higher stability [55].…”

Section: Xps Analyses Of Biominerals Induced By Synechocystis Sp Pcc ...mentioning

confidence: 98%

“…Therefore, a sufficiently high concentration of Mg 2+ ions does indeed inhibit CA activity. It has been reported that CA is a metalloenzyme containing Zn 2+ at its biologically active sites, and this essential metal ion can be substituted with other metals such as Fe 3+ [55]. Interestingly, when Fe 3+ replaces the native Zn 2+ , it significantly enhances enzyme activity by modifying the enzymes by metal ion replacement.…”

Section: Xps Analyses Of Biominerals Induced By Synechocystis Sp Pcc ...mentioning

confidence: 99%

Effect of Magnesium and Ferric Ions on the Biomineralization of Calcium Carbonate Induced by Synechocystis sp. PCC 6803

Zhao,

Han,

Liang

et al. 2023

Minerals

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The discovery of cyanobacteria fossils in microbialite prompts the investigation of carbonate biomineralization using cyanobacteria. However, the impact of coexisting magnesium and iron in microbialite on carbonate biomineralization has been overlooked. Here, Synechocystis sp. PCC 6803 was used to induce calcium carbonate in the presence of coexisting magnesium and ferric ions. The findings demonstrate that cell concentration, pH, carbonic anhydrase activity, and carbonate and bicarbonate concentrations decreased with increasing concentrations of magnesium and calcium ions. Ferric ions yielded a contrasting effect. The levels of deoxyribonucleic acid, protein, polysaccharides, and humic substances in extracellular polymeric substances increased in the presence of separated or coexisting calcium, magnesium, and ferric ions. Magnesium ions inhibited calcium ion precipitation, whereas ferric ions exhibited the opposite effect. Protein secondary structures became more abundant and O-C=O and N-C=O contents increased with increasing ion concentrations by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses. Scanning electron microscopy revealed that ferric ions lead to rougher surfaces and incomplete rhombohedral structures of calcite, whereas magnesium ions promoted greater diversity in morphology. Magnesium ions enhanced the incorporation of ferric ions. This work aims to further understand the effect of magnesium and ferric ions on calcium carbonate biomineralization induced by cyanobacteria.

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A Thermodynamic Study on the Binding of Cobalt(II) and Iron(III) Ions with Bovine Carbonic Anhydrase II at Different Temperatures

Cited by 4 publications

References 12 publications

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

Calorimetric Study on the Binding of Lysozyme Upon Interaction With β-Cyclodextrin

Effect of Magnesium and Ferric Ions on the Biomineralization of Calcium Carbonate Induced by Synechocystis sp. PCC 6803

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