A Three-Year Study of
            <i>Campylobacter jejuni</i>
            Genotypes in Humans with Domestically Acquired Infections and in Chicken Samples from the Helsinki Area

Hänninen, Marja Liisa; Perko-Mäkelä, Päivikki; Pitkälä, Anna; Rautelin, Hilpi

doi:10.1128/jcm.38.5.1998-2000.2000

Cited by 99 publications

(40 citation statements)

References 10 publications

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“…These strains were noted several times both spatially and temporally, some identified in stores belonging to different supermarket chains. This finding is consistent with data obtained by Hanninen et al (2000) and Madden et al (1998) where certain recurrent patterns were noted over a 1 or 3-year period. Further study to determine the pathogenicity of these Campylobacter strains will shed light on the epidemiology of Campylobacter and underscore the public health significance of Campylobacter as a food-borne pathogen.…”

Section: Discussionsupporting

confidence: 92%

“…Again, we do not have sufficient data to conclude that certain Campylobacter clones are host or niche specific. Studies looking at genotypes of Campylobacter from either animal or human sources did identify some strain types appearing to be associated only with humans, while other types are restricted to poultry (Koenraad et al 1995); however, most studies showed identical clones of Campylobacter could infect humans, poultry and cattle Hanninen et al 2000;Nielsen et al 2000;Fitzgerald et al 2001;Schouls et al 2003;Broman et al 2004).…”

Section: Discussionmentioning

confidence: 99%

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Genotyping of Campylobacter spp. from retail meats by pulsed-field gel electrophoresis and ribotyping

Girard

Zhao

et al. 2006

J Appl Microbiol

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Aims: To determine the genetic relatedness of Campylobacter spp. from retail meat products, and compare the discriminatory power of pulsed‐field gel electrophoresis (PFGE) and automatic ribotyping. Methods and Results: A total of 378 Campylobacter isolates recovered from 159 raw meats (130 chicken, 25 turkey, three pork and one beef) sampled from 50 retail grocery stores of four supermarket chains in the Maryland suburban area from August 1999 to July 2000 were analysed by PFGE with SmaI, 120 isolates of which were also characterized by ribotyping with PstI using RiboPrinter® system. A total of 148 unique PFGE patterns were identified, 91 of which were present in multiple Campylobacter isolates and 24 in multiple meat samples. Nineteen Campylobacter clones with identical PFGE patterns recurred frequently (up to nine times) throughout the sampling period. Comparing ribotyping with PFGE, we identified 44 PFGE patterns and 22 RiboGroups among the 120 isolates tested. Multiple PFGE patterns within one RiboGroup were commonly observed, as well as multiple RiboGroups within one PFGE pattern. Conclusions: Although Campylobacter present in retail meats were genetically diverse, certain clones persisted in poultry meats. PFGE had a greater discriminatory power than ribotyping, and the two methods were complementary in genotyping Campylobacter. Significance and Impact of the Study: Genomic DNA fingerprinting of Campylobacter confirmed diverse and recurrent Campylobacter clones in the retail meats, which provides additional data for a better understanding of the epidemiological aspect of Campylobacter infection.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Genotyping of Campylobacter spp. from retail meats by pulsed-field gel electrophoresis and ribotyping

Girard

Zhao

et al. 2006

J Appl Microbiol

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“…In this study, the human and poultry isolates of C.jejuni shared common PFGE patterns: SJ2KJ2, SJ6KJ6, SJ20KJ23 or SJ23KJ7. Because of the low number of human isolates, it is difficult to draw firm conclusions about the potential for transmission from broiler chickens to humans, as is the case in other countries (Hanninen et al 2000;. In Senegal, many enteritis sufferers do not seek medical attention and, even among those that do, only some will have a stool specimen cultured for enteric pathogens.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization and antibiotic resistance of Campylobacter spp. isolated from poultry and humans in Senegal

Cardinale

Rose²,

Gros-Claude

et al. 2006

J Appl Microbiol

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Aims: The main objectives of this study were to investigate the diversity of Campylobacter genotypes circulating in Senegal and to determine the frequency of antibiotic resistance. Methods and Results: Strains of Campylobacter jejuni isolated from poultry (n = 99) and from patients (n = 10) and Campylobacter coli isolated from poultry (n = 72) were subtyped by pulsed‐field gel electrophoresis (PFGE). The pulsotypes obtained after digestion by SmaI and KpnI revealed a significant genetic diversity in both species, but without any predominant pulsotypes. However, farm‐specific clones were identified in the majority of poultry houses (76·5%). Human and poultry isolates of C. jejuni had common PFGE patterns. High quinolone‐resistance rates were observed for C. jejuni (43·4%) and C. coli (48·6%) isolates obtained from poultry. Conclusions: The results showed a genetic diversity of Campylobacter between farms indicating multiple sources of infection; but specific clones had the ability to colonize the broiler farms. The antimicrobial resistance patterns were not related to any specific PFGE pattern suggesting that resistance was due to the selective pressure of antibiotic usage. Campylobacter with similar genotypes were circulating in both human and poultry. Significance and Impact of the Study: This study is important for the understanding of the epidemiology of Campylobacter in broiler farms in Senegal. It also emphasizes the need for a more stringent policy in the use of antimicrobial agents in food animals.

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“…Several world-wide studies have indicated that poultry meats are commonly contaminated with Campylobacter. Reported rates of contamination include 36% in Denmark [14], 81.3% in Italy [15], 83.3% in the UK [16], 45% in Australia [17], 40% in Canada [18], 10-33% in Finland [19], and 70.7% in the US [20]. Ono and Yamamoto [2] reported a contamination rate of 45.8% among retail domestic poultry meat in Saitama, Japan, while here we found that 71.2% of retail poultry meat purchased in Akita, Japan, was positive for C. jejuni.…”

Section: Discussionmentioning

confidence: 99%

Campylobacter jejuniisolated from retail poultry meat, bovine feces and bile, and human diarrheal samples in Japan: Comparison of serotypes and genotypes

Saito

Yatsuyanagi

Harata

et al. 2005

FEMS Immunology &Amp; Medical Microbiology

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To determine the significance of poultry and bovine as infectious sources of Campylobacter jejuni in Japan, the serotype distribution and pulsed-field gel electrophoresis (PFGE) patterns of poultry and bovine isolates were compared with those of isolates from patients with diarrhea in Akita (Japan). Serotypes O:2 and O:4-complex were common in human, poultry, and bovine isolates, and serotype O:23,36,53 was common in human and bovine isolates. SmaI PFGE patterns of isolates belonging to these serotypes were generated. Eight PFGE patterns were shared by poultry and human isolates and three patterns were shared by human and bovine isolates. Further analysis of the isolates having the same SmaI PFGE pattern by KpnI PFGE confirmed that four patterns and two patterns were still shared by poultry and human isolates, and bovine and human isolates, respectively. Thus, serotypic and genotypic data indicated a possible link between sporadic human campylobacteriosis and C. jejuni from retail poultry and bovine bile and feces, suggesting that bovine serves as an infectious source of C. jejuni in Japan, as is observed in other countries.

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A Three-Year Study of Campylobacter jejuni Genotypes in Humans with Domestically Acquired Infections and in Chicken Samples from the Helsinki Area

Cited by 99 publications

References 10 publications

Genotyping of Campylobacter spp. from retail meats by pulsed-field gel electrophoresis and ribotyping

Genotyping of Campylobacter spp. from retail meats by pulsed-field gel electrophoresis and ribotyping

Genetic characterization and antibiotic resistance of Campylobacter spp. isolated from poultry and humans in Senegal

Campylobacter jejuniisolated from retail poultry meat, bovine feces and bile, and human diarrheal samples in Japan: Comparison of serotypes and genotypes

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