A threshold LC–MS/MS method for 92 analytes in oral fluid collected with the Quantisal® device

Desharnais, Brigitte; Lajoie, Marie-Jo; Laquerre, Julie; Mireault, Pascal; Skinner, Cameron D.

doi:10.1016/j.forsciint.2020.110506

Cited by 15 publications

(7 citation statements)

References 24 publications

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“…Quantisal® is also known to exhibit higher extraction recovery and good reproducibility for many drugs of abuse and drugs like antipsychotics 36–38 . However, it has been reported that compounds like polyglycols present in the stabilizing buffer can hamper the MS detection 39 . Therefore, we compared Quantisal® buffer and a homemade mix containing ACN/H 2 O (50/50 v/v) + 2% acetic acid.…”

Section: Resultsmentioning

confidence: 99%

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Simultaneous quantification of 19 nonsteroidal anti‐inflammatory drugs in oral fluid by liquid chromatography‐high resolution mass spectrometry: Application on ultratrail runner's oral fluid

Mashal

Nalin

Bévalot

et al. 2022

Drug Testing and Analysis

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Nonsteroidal anti-inflammatory drugs (NSAIDs) are a therapeutic class suspected to be used by ultratrail runners. The use of NSAIDs during ultratrails is known to be associated with various adverse effects. To study the prevalence of NSAIDs intake in ultratrail runners, oral fluid (OF) is a relevant matrix as it is noninvasive and easy to collect. The aim of our work was to develop and validate a liquid-liquid extraction followed by a liquid chromatography (LC)-mass spectrometry (MS)/high resolution mass spectrometry (HRMS) method for the simultaneous quantification of 19 NSAIDs in OF. After a comparison of different liquid-liquid extraction methods, a double step liquid-liquid extraction with chloroform was performed on OF collected with Quantisal ® , with extraction recoveries higher than 90%. An Accucore AQ column

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Section: Resultsmentioning

confidence: 99%

“…[36][37][38] However, it has been reported that compounds like polyglycols present in the stabilizing buffer can hamper the MS detection. 39 Therefore, we compared Quantisal ® buffer and a homemade mix containing ACN/H 2 O (50/50 v/v) + 2% acetic acid.…”

Section: Sample Collectionmentioning

confidence: 99%

Simultaneous quantification of 19 nonsteroidal anti‐inflammatory drugs in oral fluid by liquid chromatography‐high resolution mass spectrometry: Application on ultratrail runner's oral fluid

Mashal

Nalin

Bévalot

et al. 2022

Drug Testing and Analysis

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show abstract

“…In specific experiments it was concluded that the loss of benzodiazepines, especially FLU and clonazepam, during natural storage is mainly due to irreversible adsorption of the substances on the collection device, and that pre‐addition of methanol to the collection device can significantly improve this state and achieve maximum stability. In 2020, also employing Quantisal™ collection OF, Desharnais and coworkers established a qualitative LC‐MS/MS decision point analysis method with high throughput and greater stability to monitor and validate the presence of benzodiazepines and metabolites [63] . This method was mainly optimized for the precipitation solvent used (acetone: acetonitrile 30 : 70 V: V), which dramatically improved the stability of FLU and 7AF.…”

Section: Detection Strategy Of Flumentioning

confidence: 99%

“…In 2020, also employing Quantisal™ collection OF, Desharnais and coworkers established a qualitative LC-MS/MS decision point analysis method with high throughput and greater stability to monitor and validate the presence of benzodiazepines and metabolites. [63] This method was mainly optimized for the precipitation solvent used (acetone: acetonitrile 30 : 70 V: V), which dramatically improved the stability of FLU and 7AF.…”

Section: Conventional Lc-ms-based Detection Systemmentioning

confidence: 99%

Detection of Flunitrazepam in Biological Fluids and Beverages:A Review

Chen,

Huang,

Qiu

et al. 2023

ChemistrySelect

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Flunitrazepam, a very potent benzodiazepine with sedative, hypnotic and amnesic effects, is commonly used to treat severe insomnia and as an anesthetic. Due to these characteristics, when flunitrazepam is sneaked into alcohol or drinks, it is often highly associated with violent behavior and drug rape cases. Therefore, it is also known as a “date rape drug”, and the detection of flunitrazepam and its metabolites is of great interest in forensic medicine and pharmacotoxicology. This paper describes the mechanism of action and drug metabolism process of flunitrazepam, reviews the different methods for detecting flunitrazepam published over the years, summarizes the detection limits of each method and compares their advantages and disadvantages. We hope to provide some inspiration for future research in this field.

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“…However, OF analysis faces the same challenges observed for conventional biosamples, namely: the low concentration of the analytes and the high presence of interferents. 15 The extraction of TCAs from OF by solid-phase extraction 16 and liquid–liquid extraction 17 has been reported as suitable approaches to overcome both issues.…”

Section: Introductionmentioning

confidence: 99%

Polydopamine coated hypodermic needles as a microextraction device for the determination of tricyclic antidepressants in oral fluid by direct infusion MS/MS

et al. 2021

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A threshold LC–MS/MS method for 92 analytes in oral fluid collected with the Quantisal® device

Cited by 15 publications

References 24 publications

Simultaneous quantification of 19 nonsteroidal anti‐inflammatory drugs in oral fluid by liquid chromatography‐high resolution mass spectrometry: Application on ultratrail runner's oral fluid

Simultaneous quantification of 19 nonsteroidal anti‐inflammatory drugs in oral fluid by liquid chromatography‐high resolution mass spectrometry: Application on ultratrail runner's oral fluid

Detection of Flunitrazepam in Biological Fluids and Beverages:A Review

Polydopamine coated hypodermic needles as a microextraction device for the determination of tricyclic antidepressants in oral fluid by direct infusion MS/MS

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