A tissue-engineered trachea derived from a framed collagen scaffold, gingival fibroblasts and adipose-derived stem cells

Kobayashi, Ken; Suzuki, Teruhisa; Nomoto, Yukio; Tada, Yasuhiro; Miyake, Masakazu; Hazama, Akihiro; Wada, Ikuo; Nakamura, Tatsuo; Omori, Koichi

doi:10.1016/j.biomaterials.2010.02.027

Cited by 104 publications

(87 citation statements)

References 37 publications

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“…These functions can be temporarily or permanently compromised in patients with airway stenosis and malignant tumors or after tracheal resection 20. Hence, one of the main challenges for the translational application of tissue‐engineered tracheas is the ability to efficiently restore a functional epithelial cell layer.…”

Section: Discussionmentioning

confidence: 99%

A Modular Strategy to Engineer Complex Tissues and Organs

et al. 2018

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Currently, there are no synthetic or biologic materials suitable for long‐term treatment of large tracheal defects. A successful tracheal replacement must (1) have radial rigidity to prevent airway collapse during respiration, (2) contain an immunoprotective respiratory epithelium, and (3) integrate with the host vasculature to support epithelium viability. Herein, biopolymer microspheres are used to deliver chondrogenic growth factors to human mesenchymal stem cells (hMSCs) seeded in a custom mold that self‐assemble into cartilage rings, which can be fused into tubes. These rings and tubes can be fabricated with tunable wall thicknesses and lumen diameters with promising mechanical properties for airway collapse prevention. Epithelialized cartilage is developed by establishing a spatially defined composite tissue composed of human epithelial cells on the surface of an hMSC‐derived cartilage sheet. Prevascular rings comprised of human umbilical vein endothelial cells and hMSCs are fused with cartilage rings to form prevascular–cartilage composite tubes, which are then coated with human epithelial cells, forming a tri‐tissue construct. When prevascular– cartilage tubes are implanted subcutaneously in mice, the prevascular structures anastomose with host vasculature, demonstrated by their ability to be perfused. This microparticle–cell self‐assembly strategy is promising for engineering complex tissues such as a multi‐tissue composite trachea.

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Section: Discussionmentioning

confidence: 99%

A Modular Strategy to Engineer Complex Tissues and Organs

et al. 2018

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“…In vivo ectodermal capacity of ASCs is also indicated by their regeneration of epithelial tissues. ASCs, combined with scaffolds, and implanted into rat tracheal defects show the development of a pseudostratified epithelium with goblet cells [283,284]. In addition, a large field of research has developed suggesting the use of ASCs in epidermal and/or dermal regeneration.…”

Section: In Vivo Ectoderm Regeneration-epithelial and Epidermalmentioning

confidence: 99%

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

2013

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In 2001, researchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue. These stem cells, now known as adipose-derived stem cells or ADSCs, have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative medicine. As of today, thousands of research and clinical articles have been published using ASCs, describing their possible pluripotency in vitro, their uses in regenerative animal models, and their application to the clinic. This paper outlines the progress made in the ASC field since their initial description in 2001, describing their mesodermal, ectodermal, and endodermal potentials both in vitro and in vivo, their use in mediating inflammation and vascularization during tissue regeneration, and their potential for reprogramming into induced pluripotent cells.

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“…Mostafa et al have reported that human gingival fibroblasts (HGFs) can be induced to differentiate into osteocytes in vitro (Mostafa et al, 2011). In addition, heterotopic gingival fibroblasts have been used as transplanted cells to facilitate tracheal epithelial regeneration (Kobayashi et al, 2007(Kobayashi et al, , 2010, periodontal tissue regeneration (Nakajima et al, 2008), and skin wound healing (Nishi et al, 2010). Moreover, HGFs display immunosuppressive effects on T-lymphocytes similar to those of periodontal ligament stem cells (Wada et al, 2009).…”

Section: Gmscs Vs Gingival Fibroblastsmentioning

confidence: 99%

Human Oral Mucosa and Gingiva

et al. 2012

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Mesenchymal stem cells (MSCs) represent a heterogeneous population of progenitor cells with self-renewal and multipotent differentiation potential. Aside from their regenerative role, extensive in vitro and in vivo studies have demonstrated that MSCs are capable of potent immunomodulatory effects on a variety of innate and adaptive immune cells. In this article, we will review recent experimental studies on the characterization of a unique population of MSCs derived from human oral mucosa and gingiva, especially their immunomodulatory and anti-inflammatory functions and their application in the treatment of several in vivo models of inflammatory diseases. The ease of isolation, accessible tissue source, and rapid ex vivo expansion, with maintenance of stable stem-celllike phenotypes, render oral mucosa-and gingivaderived MSCs a promising alternative cell source for MSC-based therapies.

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A tissue-engineered trachea derived from a framed collagen scaffold, gingival fibroblasts and adipose-derived stem cells

Cited by 104 publications

References 37 publications

A Modular Strategy to Engineer Complex Tissues and Organs

A Modular Strategy to Engineer Complex Tissues and Organs

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

Human Oral Mucosa and Gingiva

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